Addgene

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Sa-SchCas9

Plasmid

#192137

Purpose

Expresses Sa-SchCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al Adv Sci (Weinh). 2022 Feb;9(4):e2104789.

Insert

Sa-SchCas9

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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J23101

Plasmid

#55176

Purpose

Very weak constitutive promoter

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

J23101 promoter

Use

Synthetic Biology; Bacillus biobrick box

Tags

Expression

Mutation

Promoter

J23101

Available since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-hM3D(Gq)::RAM-d2tTA

Plasmid

#189627

Purpose

Expresses hM3D(Gq) under the hSyn promoter, and d2tTA under the pRAM (synthetic Fos) promoter. Plasmid 1 for recording Fos expression.

Depositor

Jerzy Szablowski

Article

Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Inserts

hM3D(Gq)
d2tTA

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

Promoter

hSyn and pRAM

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfPENK-0.9k-Venus-WPRE

Plasmid

#156401

Purpose

Expresses Venus under PENK gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcFLAG-CSK-R107E

Plasmid

#74503

Purpose

Overexpression of CSK R107E mutant in mammalian cells

Depositor

Lars Rönnstrand

Article

Kazi et al Cell Signal. 2013 Sep;25(9):1852-60. doi

Insert

CSK (CSK Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

R107E, K222R

Promoter

CMV

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pET28a-Beclin1 (1-242)

Plasmid

#24401

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains amino acids 1-242 only.

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pFX-β-tubulin-Sirius

Plasmid

#175825

Purpose

Express Sirius with microtubule-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with microtuble-targeting sequence

Use

Tags

human tubulin beta 4B class IVb;TUBB4B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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p148

Plasmid

#62616

Purpose

ePathBrick pETM6 vector encoding CsF3H, AaDFR, and DuLAR

Depositor

Mattheos Koffas

Article

Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

Inserts

flavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase

Use

Synthetic Biology

Tags

GBD, PDZ, and SH3

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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(517) SB KRAB-SadCas9 U6-gStop

Plasmid

#163023

Purpose

Sleeping Beauty TET-On expression of CRISPRi with gSTOP gRNA

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Mammalian codon-optimized SadCas9

Use

Transposon

Tags

KRAB and myc NLS

Expression

Mammalian

Mutation

Promoter

Tet-On

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pPSP6+t-gfp

Plasmid

#48130

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-SP6-inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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gRNA_tdtomato_reporter_1_SA-2x

Plasmid

#79369

Purpose

Assays activity of transcriptional activators fused to SA Cas9

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

tdtomato

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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1120 pBabe puroL HA FKHR H215R

Plasmid

#9026

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

FKHR H215R (FOXO1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

H215R

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pCS2 Flag Smad3 T179V

Plasmid

#26997

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Expression

Mutation

T179V

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pTYF-super-mfCCK-0.5k-Venus-WPRE

Plasmid

#156392

Purpose

Expresses Venus under CCK gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Vrk2

Plasmid

#120240

Purpose

Expresses V5-tagged Vrk2 in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Vrk2 (Vrk2 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pEntr1a-mcherry-FKBP-TIAM1(DHPH)

Plasmid

#176135

Purpose

Heterodimerization with FRB (rapamycin), Rac1 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

TIAM1 (TIAM1 Human)

Use

Entry vector

Tags

Expression

Mutation

See Depositor Comments Below

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV FLIPi P2G_Thy1.1 Dbl (p53,PTEN)

Plasmid

#19746

Depositor

Richard Hynes

Article

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting p53 and PTEN (Pten Mouse)

Use

Cre/Lox, RNAi, and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

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pET41a iGluf

Plasmid

#119833

Purpose

Production of recombinant iGluSnFR E25D fast variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluf

Use

Tags

GST

Expression

Bacterial

Mutation

E25D

Promoter

T7

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pSB96 - pL2_pSB90_2x35S::fLUC-I::tNOS

Plasmid

#123188

Purpose

binary plant vector for transient fLUC (with intron) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::fLUC-I::tNOS and VirGN54D in the vector backbone

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSN69

Plasmid

#100125

Purpose

expresses putative cMYC DBD with a HA tag

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCerulean-P2A-putative cMYC DBD with a HA tag (MYC Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Insert corresponds to the DBD of cMYC

Promoter

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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