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Plasmid#55183PurposecMyc tagDepositorInsertcMyc tag
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pMC-6-AtSpCas9-9
Plasmid#197778PurposePhytobrick (MoClo) Level 0 PartDepositorInsertAtSpCas9
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-AtU6-Y
Plasmid#197779PurposePhytobrick (MoClo) Level 0 PartDepositorInsertAtU6 promoter for sgRNA assembly
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-OsU6-X
Plasmid#197780PurposePhytobrick (MoClo) Level 0 PartDepositorInsertOsU6 promoter for sgRNA assembly
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-Z-SpCas9-gRNA-repeat-polyT-11
Plasmid#197781PurposePhytobrick (MoClo) Level 0 PartDepositorInsertgRNA repeat for U6 sgRNA assembly
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-myrSNAP-CON
Plasmid#60099PurposeExpresses membrane-targeted SNAP(f)-tag and histone-GFP, conditionalDepositorInsertsSNAP-tag
histone-GFP
UseAAV and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCaSper4 cyto roGFP2-Orp1
Plasmid#64998PurposeExpression of cytosolic roGFP2-Orp1 under the alphaTub84B promoterDepositorInsertOrp1 (HYR1 Budding Yeast)
UseTagsroGFP2ExpressionInsectMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKMV-VioE
Plasmid#65351Purposesysthesis of vioE gene in the violacein pathwayDepositorInsertvioE
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
px458_2A_GFP_sgRNA_NEMF
Plasmid#127126DepositorInsertgRNA NEMF (NEMF Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTrex-Luc-Phleo
Plasmid#48337PurposeTrasgenically expresses firefly luciferase in T. cruzi with phleomycin selectionDepositorInsertfirely luciferase
UseTrypanasoma cruzi expressionTagsExpressionMutationPromoterT. cruzi rRNAAvailable sinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL1.7XX
Plasmid#49344Purposeluciferase reporter with mouse Bmp2 promoterDepositorInsertBmp2 distal promoter (Bmp2 Mouse)
UseLuciferaseTagsluciferaseExpressionMutationnt –1237 to +471PromoterBmp2 distal promoterAvailable sinceDec. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJK357
Plasmid#71568PurposeProduces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase (AarC)DepositorInsertsuccinyl-CoA:acetate CoA-transferase
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mWasabi-ER
Plasmid#164165PurposeExpresses mWasabi fused with an ER-targeting sequence of calreticulin and the ER retrieval sequence, KDEL, under the control of CAG promoterDepositorInsertmWasabi
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-Cre-GR-9
Plasmid#197723PurposePhytobrick (MoClo) Level 0 PartDepositorInsertCre recombinase with St-LS1 intron fused to glucocorticoid receptor
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
hP14Gal4
Plasmid#84364PurposeGal4-PRDM14 funsion plasmid for luciferase recruitment assayDepositorInsertPRDM14 (PRDM14 Human)
UseTagsGal4 and HA tagExpressionMammalianMutationsilent mutations at coding silent 1068CtoT, 1629T…PromoterCMV/TOAvailable sinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSIBR002
Plasmid#177665PurposeSIBR Intron 2 (Int2) interrupts the CDS of FnCas12aDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterlacUV5Available sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A3701
Plasmid#91052PurposeModule A, Promoter: 35S, Gene: Csy4-P2A-dAtCas9-VPR, Terminator: HSPDepositorInsertCsy4-P2A-dAtCas9-VPR
UseCRISPRTagsExpressionMutationPromoter35SAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
Dimer cleaved
Plasmid#198199PurposeFRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavageDepositorInsertmVenus-T2A-mTurquoise2
UseTagsExpressionMammalianMutationA206K bothPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
2X_pX458_pSpCas9(BB)-2A-GFP_NANOG_bKO
Plasmid#172224PurposeCas9-2A-GFP expression vector bearing two sgRNAs targeting the telomeric human NANOG CTCF-boundary.DepositorInsertsgRNA
UseCRISPRTags3XFLAG and GFPExpressionMammalianMutationPromoterCbhAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only