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Plasmid#68178PurposeProvides the 5'-untranslated region of the Arabidopsis thaliana actin2 gene as a level 0 GoldenBraid partDepositorInsert5'UTR AtAct2
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLAG-SBP-STAG2 (Y1142fs)
Plasmid#73969PurposeSTAG2 cDNA (CCDS43990) Y1142fsDepositorInsertSTAG2 (STAG2 Human)
UseTagsFLAG-SBPExpressionMammalianMutationY1142fsPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLAG-SBP-STAG2 (I885fs)
Plasmid#73966PurposeSTAG2 cDNA (CCDS43990) I885fsDepositorInsertSTAG2 (STAG2 Human)
UseTagsFLAG-SBPExpressionMammalianMutationI885fsPromoterAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLAG-SBP-STAG2 (S202L)
Plasmid#73972PurposeSTAG2 cDNA (CCDS43990) S202LDepositorInsertSTAG2 (STAG2 Human)
UseTagsFLAG-SBPExpressionMammalianMutationS202LPromoterAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLAG-SBP-STAG2 (V181M)
Plasmid#73971PurposeSTAG2 cDNA (CCDS43990) V181MDepositorInsertSTAG2 (STAG2 Human)
UseTagsFLAG-SBPExpressionMammalianMutationV181MPromoterAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tg 2 kb Violet opsin GFP
Plasmid#72921Purposeviolet opsin promoter from zebra finch (Taeniopygia guttata) gDNA (nucleotides21,946 to 145, corresponding to chr1A_ran-dom:206453–208444 in taeGut1) driving GFPDepositorInsertviolet opsin promoter
UseTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tg 2 kb Violet opsin DsRed
Plasmid#72920Purposeviolet opsin promoter from zebra finch (Taeniopygia guttata) gDNA (nucleotides21,946 to 145, corresponding to chr1A_ran-dom:206453–208444 in taeGut1) driving DsRedDepositorInsertviolet opsin promoter
UseTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ac 1.8kb red opsin GFP.
Plasmid#72915Purposered opsin promoter from Carolina anole lizard (Anolis carolinensis) gDNA (nucleotides21,797 to 187, corresponding to chr2:88663108–88664997 in anoCar2.0) driving GFPDepositorInsertred opsin promoter
UseTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ac 1.8kb red opsin DsRed
Plasmid#72914Purposered opsin promoter from Carolina anole lizard (Anolis carolinensis) gDNA (nucleotides21,797 to 187, corresponding to chr2:88663108–88664997 in anoCar2.0) driving DsRedDepositorInsertred opsin promoter
UseTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Gg 1.8kb rhodopsin GFP
Plasmid#72917PurposeRhodopsin promoter from chicken (Gallus gallus) gDNA chr12:19,499,638–19,501,514 in galGal4 driving GFPDepositorInsertchicken rhodopsin (RHO Chicken)
UseTagsExpressionMutationPromoterAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
Gg 1.8kb rhodopsin DsRed
Plasmid#72916PurposeRhodopsin promoter from chicken (Gallus gallus) gDNA chr12:19,499,638–19,501,514 in galGal4 driving DsRedDepositorInsertchicken rhodopsin (RHO Chicken)
UseTagsExpressionMutationPromoterrhodopsinAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGB 1alfa1R Tnos:Hygromycin:PNos (GB0235)
Plasmid#68213PurposeTranscriptional unit for Hygromycin resistance gene plant expressionDepositorInsertHyg
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
mSTIM1_1-250-CFP
Plasmid#69552PurposeExpresses a STIM1_1-250 fragment mammalian cellsDepositorInsertmSTIM1_1-250
UseTagsCyan Fluorescent Protein (CFP)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDGB1omega1_SF (GB0158)
Plasmid#68242PurposeTwister plasmid to swap inserts from an omega2 or omega2R vector to any alpha level vectorDepositorInsertSolanum lycopersicum intergenic region
UseSynthetic Biology; UnspecifiedTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMyb12 (GB0075)
Plasmid#68199PurposeProvides the CDS of theSolanum lycopersicum Myb12 transcription factor as a level 0 GoldenBraid partDepositorInsertMyb12 coding region
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPAt2S3 (GB0029)
Plasmid#68162PurposeProvides the Arabidopsis thaliana Seed-specific promoter 2S3 as a level 0 GoldenBraid partDepositorInsertAt2S3 promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUPD_Mini35s(-60,UTR) (GB0050)
Plasmid#68176PurposeProvides the CaMV Mini35S Promoter as a level 0 GoldenBraid partDepositorInsertCaMV Mini35S promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pES007 (pTet-qacR 5/pQacA-deGFP)
Plasmid#69036Purposereporter with qacR 5 on single plasmidDepositorInsertsUseTagsHis6 tagExpressionBacterialMutationW61Y, E90Q, F102S, M116Q, Y119L, E120QPromoterpQacA and pTetAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pES003 (pTet-qacR 2)
Plasmid#69032PurposeqacR 2 downstream of a Tet promoterDepositorInsertqacR (qacR Synthetic)
UseTagsHis6 tagExpressionBacterialMutationE57Q, E58L, W61Y, E90Q, I99Q, M116Q, L119Y, E120Q…PromoterpTetAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
NET31 mRFP-N2 (490)
Plasmid#61984Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET31 (TMEM209 Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only