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PGKp-GFP-YAP (S94A)

Plasmid

#174173

Purpose

Lentiviral vector to constitutively express TEAD-binding mutant YAP under control of the human PGK promoter.

Depositor

Rod Bremner

Article

Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

Insert

YAP (YAP1 Human)

Use

Lentiviral

Tags

3x FLAG tag

Expression

Mammalian

Mutation

Serine 94 mutated to Alanine

Promoter

PGK

Available since

Aug. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pFA6a-kanMX6-PGAL1-GFP

Plasmid

#41614

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Expression

Mutation

Promoter

Available since

Dec. 11, 2012

Availability

Academic Institutions and Nonprofits only

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pMB1-spect-PthrC3_8-eGFP

Plasmid

#107411

Purpose

This plasmid contain promoter PthrC3_8, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

Depositor

Kang Zhou

Article

Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

Insert

eGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PthrC3_8

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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attB-GFP-Dnmt3b1-Poly(A)

Plasmid

#65531

Purpose

Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b1 cDNA into a MIN-tagged locus

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Dnmt3b (Dnmt3b Mouse)

Use

Mouse Targeting; Bxb1

Tags

GFP

Expression

Mammalian

Mutation

isoform 1

Promoter

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a_DmKHC[1-421]-1xGFP-6xHis

Plasmid

#196972

Purpose

Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tag

Depositor

Lukas Kapitein

Article

Tas et al Neuron. 2017 Dec 20;96(6):1264-1271.e5.

Insert

Kinesin Heavy Chain (Khc Fly)

Use

Tags

6xHis and GFP

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-puro-eGFP-HEC1-9A

Plasmid

#114023

Purpose

Inducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1 - 9A (NDC80) (NDC80 Human)

Use

Tags

Expression

Mammalian

Mutation

Hec1 - 9A

Promoter

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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LLP109 pEF1a-gRNA-GFP

Plasmid

#100935

Purpose

GFP, puromycin and gRNA scaffold driven by EF1a promoter

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

gRNA scaffold

Use

Grna scaffold

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pT2KXIG (Tol2)-tp63:ikk1KD:GFP

Plasmid

#105645

Purpose

Kinase-deleted Ikk1(alpha) expression in basal keratinocytes

Depositor

Sandra Rieger

Article

Lisse et al J Cell Sci. 2017 Mar 1;130(5):975-988. d

Insert

tp63:ikk1KD:GFP (chuk Zebrafish)

Use

Tags

GFP

Expression

Mammalian

Mutation

p63 promoter + ikk1 (kinase domain deleted) + GFP

Promoter

Available since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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CAG.3xHalo-GFP-SPC25

Plasmid

#176206

Purpose

Kinetochore anchor for TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

3xHalo-GFP-SPC25

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pMY-eGFP-P2A-PuroR-HA

Plasmid

#163343

Purpose

Retroviral vector to express cytosolic GFP and an HA-tagged puromycin selection marker

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Insert

GFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pC28-LIC-sfGFP-His

Plasmid

#165479

Purpose

pET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tag

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Use

Tags

TEV-GFP-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-nl.EGFP-jRGECO1a

Plasmid

#172386

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

nuclear-localized EGFP + jRGECO1a

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Bgh-GFP-Farpl

Plasmid

#115501

Purpose

expression of rat Farp1

Depositor

Thomas Biederer

Article

Cheadle et al J Cell Biol. 2012 Dec 10;199(6):985-1001

Insert

Farp1 (Farp1 Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

chicken beta-actin

Available since

Dec. 26, 2018

Availability

Academic Institutions and Nonprofits only

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bdSUMO-HA2-GFP-HiBit

Plasmid

#171017

Purpose

For bacterial expression of bdSUMO tagged muGFP with HA2 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-HA2-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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EFS eGFP hPGK BSD

Plasmid

#188520

Purpose

Backbone for CRISPR interference (CRISPRi) isogenic PAM testing

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Type

Empty backbone

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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PB CAG-sfGFP-iCre-ERT2

Plasmid

#137856

Purpose

CAG-sfGFP-iCre-ERT2 piggyBac transposon

Depositor

Mario Capecchi

Insert

sfGFP-iCre-ERT2

Use

Cre/Lox; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2020

Availability

Academic Institutions and Nonprofits only

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SOX2 KO (T2A-H2B-EGFP)

Plasmid

#167969

Purpose

SOX2 CDS replacement with T2A-H2B-EGFP

Depositor

Emma Rawlins

Article

Sun et al Elife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

SRY-Box Transcription Factor 2 (SOX2 Human)

Use

CRISPR; Donor template

Tags

H2B-EGFP

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Promoter

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP[D134TAG+N150TAA]

Plasmid

#164582

Purpose

superfolder GFP with D134TAG and N150TAA mutations

Depositor

Ryan Mehl

Article

Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

Insert

sfGFP-134TAG+150TAA

Use

Tags

6xHis

Expression

Bacterial

Mutation

D134TAG + N150TAA Mutations

Promoter

T7

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEBB-II-I-GFP alpha isoform

Plasmid

#22146

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-HSD17B11-V16D-FLAGC

Plasmid

#184500

Purpose

Expresses the V16D mutant of human HSD17B11 with a C-terminal FLAG-GFP tag. Confers G418 resistance.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

17-beta-hydroxysteroid dehydrogenase 11 (HSD17B11 Human)

Use

Tags

FLAGC-GFP

Expression

Mammalian

Mutation

V16D

Promoter

CMV

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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