Addgene

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pFX-EGFP-KRasCT

Plasmid

#175833

Purpose

Express EGFP with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with plasma membrane-targeting sequence

Use

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-RiboJ-mScarlet-T7

Plasmid

#173227

Purpose

Encodes mScarlet fluorescent protein. To be used in cell-free system

Depositor

Jim Haseloff

Article

Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

Insert

mScarlet

Use

Cell-free system

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-N1-Barkor

Plasmid

#24402

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Barkor (ATG14 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2010

Availability

Academic Institutions and Nonprofits only
pUPD2 PhiC31 integrase (GB1496)

Plasmid

#160578

Purpose

Streptomyces phage PhiC31 integrase, complete CDS

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

PhiC31

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1alpha-HA-TFAP4

Plasmid

#110158

Purpose

Lentiviral vector for expression of human TFAP4 in mammalian cells

Depositor

Daniele Guardavaccaro

Article

D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

Insert

TFAP4 (transcription factor AP-4) (TFAP4 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMIG-Flag-RIPK1-D324A

Plasmid

#102678

Purpose

retroviral expression plasmid for RIPK1-D324A

Depositor

Hasam Habelhah

Article

Zhang et al Mol Cell Biol. 2015 Oct;35(19):3324-38.

Insert

RIPK1 (RIPK1 Human)

Use

Retroviral

Tags

Flag and IRES-GFP

Expression

Mammalian

Mutation

CHANGE Glutamate 324 to Alanine

Promoter

unknown

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pBacPAK8-His-myc-Rbx1

Plasmid

#29506

Depositor

Joan Conaway, Ronald Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Rbx1 (Rbx1 Mouse)

Use

Tags

His and myc

Expression

Insect

Mutation

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pTYF-super-mfPV-0.8k-Venus-WPRE

Plasmid

#156399

Purpose

Expresses Venus under PV gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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mEos3-caRac1

Plasmid

#92041

Purpose

Expresses mEos3 fusion with caRac1 (Q61L)

Depositor

Ji Yu

Article

Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

Insert

RAC1 (RAC1 Human)

Use

Tags

mEos3

Expression

Bacterial, Insect, and Mamm…

Mutation

Q61L (constitutively active)

Promoter

CMV

Available since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pGST-Fah_W

Plasmid

#163089

Purpose

Expressing mouse wildtype FAH in bacterial cells

Depositor

Joseph Takahashi

Article

Yang et al Proc Natl Acad Sci U S A. 2019 Oct 29;11

Insert

Fah_W

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-Fah_W

Plasmid

#163087

Purpose

expressing mouse wild type FAH in mammalian cells

Depositor

Joseph Takahashi

Article

Yang et al Proc Natl Acad Sci U S A. 2019 Oct 29;11

Insert

Fah_W

Use

Tags

Myc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2 Flag Smad3 S204A

Plasmid

#27114

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Expression

Mutation

S204A

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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1121 pcDNA3 GFP FKHR H215R

Plasmid

#9027

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

FKHR H215R (FOXO1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

H215R

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pCEP4-HA-MEKK1-1-1493

Plasmid

#21632

Depositor

Melanie Cobb

Article

Xu et al Proc Natl Acad Sci U S A. 1995 Jul 18. 9

Insert

mitogen-activated protein kinase kinase kinase 1 (Map3k1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2010

Availability

Academic Institutions and Nonprofits only
pSB115 - pL2_pSB90_fLUC-I_tGFP-I

Plasmid

#123190

Purpose

binary plant vector for transient tGFP (with intron) and fLUC (with intron) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::tGFP-I::tNOS 2x35S::fLUC-I::tNOS

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pFX-β-tubulin-SECFP

Plasmid

#175826

Purpose

Express SECFP with microtubule-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

SECFP with microtuble-targeting sequence

Use

Tags

human tubulin beta 4B class IVb;TUBB4B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pSP64T acvr1ba*

Plasmid

#185409

Purpose

Constitutively active form of acvr1ba (Nodal receptor). For in vitro transcription (SP6)

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

acvr1ba (acvr1ba Zebrafish)

Use

In vitro synthesis of mrna

Tags

Expression

Mammalian

Mutation

changed Threonine 206 to Aspartic acid

Promoter

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pEGB3 alpha1 Pnos:PhiC31int:Tnos (GB1531)

Plasmid

#160616

Purpose

TU for the constitutive expression of Streptomyces phage PhiC31 integrase.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

PhiC31

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Sha2Cas9

Plasmid

#192140

Purpose

Expresses Sha2Cas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

Insert

Sha2Cas9

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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SpeCas9

Plasmid

#192139

Purpose

Expresses SpeCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

Insert

SpeCas9

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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