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Plasmid#50612PurposeNS module plasmid for Cermak, et al., 2011 TALEN kitDepositorInsertNS4
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNS5
Plasmid#50613PurposeNS module plasmid for Cermak, et al., 2011 TALEN kitDepositorInsertNS5
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNS6
Plasmid#50614PurposeNS module plasmid for Cermak, et al., 2011 TALEN kitDepositorInsertNS6
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNS7
Plasmid#50615PurposeNS module plasmid for Cermak, et al., 2011 TALEN kitDepositorInsertNS7
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
2R2O
Plasmid#51350DepositorInsert2R2O
UseTagsHisExpressionBacterialMutationTruncation: See CommentsPromoterT7Available sinceFeb. 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAP1-3
Plasmid#71258Purposeluciferase reporter with 3 copies of the stromelylisin gene AP-1 site in front of hGM-CSF -55 to +28 minimal promoterDepositorInsert3 copies of an AP-1 site from the human Stromelysin-1 gene (MMP3 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterGM-CSF minimalAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
mT2A-sfGFP-linker-iCre-ERT2-FNF
Plasmid#182341PurposeRecombineering cassette plasmid for T2A-sfGFP-linker-iCre-ERT2-FNFDepositorInsertT2A-sfGFP-linker-iCre-ERT2-FNF
UseMouse Targeting; Flp / frtTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXZ13lacZalpha
Plasmid#160229Purposeplaces LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal platesDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterlacAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2_RBD -N501Y
Plasmid#169407PurposeCell-free/mammalian expression vector for SARS-COV-2 mutated RBD domain (N510Y, UK strain) of S protein with N-term eGFPDepositorInsertSARS-COV-2 _RBD (N501Y) (S SARS-Cov2)
UseCell-free expression vectorTagsHis and eGFPExpressionMutationN501YPromoterT7- CMV/CAGAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
Mouse 5' Srcap AID GFP Puro
Plasmid#127903PurposeHDR (donor) Plasmid for Inserting PuroR-2A-GFP-AID into the 5' end of the Mouse Srcap GeneDepositorInsertSrcap (Srcap Mustard Weed, Mouse)
UseDonor plasmidTagsGFP, AID, PuroRExpressionMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFOXA3.1.0-gDNA
Plasmid#112417PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor FOXA3DepositorInsertFOXA3 (FOXA3 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGU08
Plasmid#212149PurposePlasmid expressing catalase-peroxidase and D-amino acid dehydrogenase under constitutive promoter PgyrA control. Best E. coli glycine utilization plasmid.DepositorInsertcatalase-peroxidase, D-amino acid dehydrogenase (katG katG: E. coli, dadA6: Cupriavidus necator)
UseTagsExpressionBacterialMutationPromoterPgyrAAvailable sinceMarch 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
INT_GFPsp
Plasmid#212144PurposePreparatory plasmid for operon integration into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein
UseTagsExpressionBacterialMutationPromoterpJ23119Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
INT_GFPsp_CamRcargo
Plasmid#212143PurposePreparatory plasmid for gene disruption into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein
UseTagsExpressionBacterialMutationPromoterpJ23119Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCell-Free_KA1_SARS-COV-2_S-frag3
Plasmid#169403PurposeCell-free/mammalian expression vector for SARS-COV-2 virus N-term fragment of S protein after the furin cleavag with N-term eGFPDepositorInsertN-term frag. S protein after the furin cleavag (N SARS-Cov2)
UseCell-free expression vectorTagsHis and eGFPExpressionMutationPromoterT7- CMV/CAGAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2_S-frag5
Plasmid#169404PurposeCell-free/mammalian expression vector for SARS-COV-2 virus C-terminal ectodomain of S protein from the furin cleavag with N-term eGFPDepositorInsertC-term frag. S protein after the furin cleavag (S SARS-Cov2)
UseCell-free expression vectorTagsHis and eGFPExpressionMutationPromoterT7- CMV/CAGAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgKcnq3
Plasmid#159911PurposeMutagenesis of Kcnq3DepositorInsertKcnq3 (Kcnq3 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgDcc
Plasmid#159906PurposeMutagenesis of DccDepositorInsertDcc (Dcc Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
LP102: pMAGIC (R4-R3) NLS-Sp Cas9-NLS
Plasmid#132927PurposepMAGIC R4-R3 entry plasmid, contains 2xNLS SpCas9 (nuclease active) for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInserthumanized SpCas9
UseCRISPR and Synthetic Biology; Pmagic gateway entr…TagsExpressionMutationPromoterAvailable sinceOct. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST PHGDH
Plasmid#154917PurposeExpresses human PHGDHDepositorInsertPHGDH (PHGDH Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only