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Showing: 801 - 820 of 18087 results

  2. C1QBP-bio-His

    Plasmid
    #53330
    Purpose
    Expresses full-length Complement component 1 Q subcomponent-binding protein ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    C1QBP (C1QBP Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53330-plasmid-map-sequence-id-83060

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  6. pCAM9

    Plasmid
    #78560
    Purpose
    pBAD30::ompU V. cholerae 395 Induce with 0.2% arabinose. Expression system: V. cholerae 395 outer membrane protein OmpU.
    Depositor
    James Kaper
    Article
    Merrell et al J Bacteriol. 2001 May;183(9):2746-54.
    Insert
    ompU
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD promoter
    Available since
    June 8, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. 6xO/S luc

    Plasmid
    #69445
    Purpose
    detection/assessment of OCT4 and SOX2 transcription factor activity
    Depositor
    Lisa Dailey
    Insert
    minimal fgf4 promoter and 6XOS tandem repeat of composite OCT4/Sox2 binding site from FGF4 enhancer
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    69445-plasmid-map-sequence-id-259015

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  14. pHL 802

    Plasmid
    #52991
    Purpose
    pLtetO-1:OxyS-mCherry, pLlacO-1:ompC::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    OxyS sRNA
    ompC
    Use
    Synthetic Biology
    Tags
    GFP and RBS-mCherry
    Expression
    Bacterial
    Mutation
    contains −81 to +36 bp relative to start codon
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pHL 1032

    Plasmid
    #52997
    Purpose
    pLtetO-1:RhyB, pCon:ompC::mCherry, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    RhyB sRNA
    ompC
    sodB
    Use
    Synthetic Biology
    Tags
    gfp and mCherry
    Expression
    Bacterial
    Mutation
    contains −56 to +141 bp relative to start codon a…
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. pHL 762

    Plasmid
    #52990
    Purpose
    pLtetO-1:MicC, pLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    MicC sRNA
    sodB
    ompC
    Use
    Synthetic Biology
    Tags
    gfp and mCherry
    Expression
    Bacterial
    Mutation
    −56 to +141 bp and −81 to +36 bp
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. pQZ210

    Plasmid
    #106414
    Purpose
    Co-expresses trans SNARE complex with pDC183 in E.coli
    Depositor
    Axel Brunger
    Article
    Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d
    Inserts
    Rat Synaptobrevin-2 fragment 28-66 (Vamp2 Synthetic)
    Syntaxin-1A fragment 191-256 (Stx1a Rat, Synthetic)
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Fragment 191-256 , Codon optimization and Fragmen…
    Promoter
    T7
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    106414-plasmid-map-sequence-id-204167

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  18. pDC183

    Plasmid
    #106415
    Purpose
    Co-expresses trans SNARE complex with pQZ210 in E. coli
    Depositor
    Axel Brunger
    Article
    Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d
    Inserts
    SNAP25 fragment 141-204 (Snap25 Rat, Synthetic)
    SNAP25 Fragment 7-83 (Snap25 Rat, Synthetic)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Fragment 141-204, Codon optimization and Fragment…
    Promoter
    T7
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    106415-plasmid-map-sequence-id-204169

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  19. pGEM-GFP-URA3-GFP

    Plasmid
    #72606
    Purpose
    Template for creating a C-terminal GFP tag AND an internal GFP tag from a single transfection of yeast
    Depositor
    Bruce Granger
    Article
    Granger et al PLoS One. 2018 Jan 12;13(1):e0191194. do
    Inserts
    GFP (full)
    URA3
    GFP (partial)
    Optional Linker
    Use
    Tags
    Expression
    Mutation
    Includes full 819 bp coding sequence of URA3, 435…
    Promoter
    Available since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    72606-plasmid-map-sequence-id-133159

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  20. pcDNA3.1.Gαi1-LgB91

    Plasmid
    #134342
    Purpose
    Nanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gαi1-LgB91 (GNAI1 Human)
    Use
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134342-plasmid-map-sequence-id-306400

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Showing: 801 - 820 of 18087 results