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Addgene
Showing: 801 - 820 of 1937 results

  1. pBD006

    Plasmid
    #201306
    Purpose
    D16: Promoter of TraesCS5D02G320700 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D16: Promoter of TraesCS5D02G320700
    Use
    Tags
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    201306-plasmid-map-sequence-id-409554

    Enlarge

  2. P1m

    Plasmid
    #120934
    Purpose
    MoClo golden gate assembly AB part plasmid for J23101 (strength 1791x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson); AB overhangs.
    Depositor
    Richard Murray
    Insert
    J23101
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120934-plasmid-map-sequence-id-235942

    Enlarge

  3. C5m

    Plasmid
    #120957
    Purpose
    MoClo golden gate assembly CD part for mRFP (monomeric red fluorescent protein; see https://doi.org/10.1073/pnas.082243699 and https://www.fpbase.org/protein/mrfp1/).
    Depositor
    Richard Murray
    Insert
    mRFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120957-plasmid-map-sequence-id-236154

    Enlarge

  4. C11m

    Plasmid
    #120959
    Purpose
    MoClo golden gate assembly CD part for Citrine (modified YFP; variant of https://www.fpbase.org/protein/citrine/) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    Citrine
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120959-plasmid-map-sequence-id-238041

    Enlarge

  5. C13m

    Plasmid
    #120960
    Purpose
    MoClo golden gate assembly CD part for Cerulean (modified CFP; variant of https://www.fpbase.org/protein/cerulean/) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    Cerulean
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120960-plasmid-map-sequence-id-245422

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  6. C32m

    Plasmid
    #120967
    Purpose
    MoClo golden gate assembly CD part for CinR (Quorum-sensing receptor for 3OC14). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ohCD carb CinR
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120967-plasmid-map-sequence-id-236380

    Enlarge

  7. C36m

    Plasmid
    #120970
    Purpose
    MoClo golden gate assembly CD part for RhlI (quorum-sensing synthase for 3OC14). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ohCD carb RhlI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120970-plasmid-map-sequence-id-245423

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  8. C40m

    Plasmid
    #120973
    Purpose
    MoClo golden gate assembly CD part for blank part (random 50bp sequence) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    Blank (Random 50bp sequence)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120973-plasmid-map-sequence-id-236027

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  9. C59m

    Plasmid
    #120976
    Purpose
    MoClo golden gate assembly CD part for mScarlet (RFP variant; see https://www.fpbase.org/protein/mscarlet/). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    mScarlet
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120976-plasmid-map-sequence-id-236030

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  10. C80m

    Plasmid
    #120992
    Purpose
    MoClo golden gate assembly CD part for mfLon protease (see doi: [10.1038/nbt.3053]). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    mfLon protease
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120992-plasmid-map-sequence-id-236095

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  11. C86m

    Plasmid
    #120997
    Purpose
    MoClo golden gate assembly CD part (phage lysis protein; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    X174E
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120997-plasmid-map-sequence-id-236126

    Enlarge

  12. C115m

    Plasmid
    #121014
    Purpose
    MoClo golden gate assembly CD part for TP901 integrase (Integrase from lactococcal phage TP901). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    TP901 integrase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    121014-plasmid-map-sequence-id-236321

    Enlarge

  13. U22m

    Plasmid
    #121025
    Purpose
    MoClo golden gate assembly BC part (high expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD6
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    121025-plasmid-map-sequence-id-245900

    Enlarge

  14. pICSL11085_GG_Wheat_live_Cas9_for

    Plasmid
    #126881
    Purpose
    Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    126881-plasmid-map-sequence-id-249630

    Enlarge

  15. pICSL11086_GG_Wheat_live_Cas9_rev

    Plasmid
    #126882
    Purpose
    Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    126882-plasmid-map-sequence-id-249649

    Enlarge

  16. pAV10.F3.loxP

    Plasmid
    #63200
    Purpose
    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    63200-plasmid-map-sequence-id-104877

    Enlarge

  17. pAV10.F6.loxP

    Plasmid
    #63201
    Purpose
    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    63201-plasmid-map-sequence-id-267056

    Enlarge

  18. pAV10.K6.loxP

    Plasmid
    #63203
    Purpose
    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    63203-plasmid-map-sequence-id-104900

    Enlarge

  19. pAV10.HO5.loxP

    Plasmid
    #63206
    Purpose
    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    63206-plasmid-map-sequence-id-104906

    Enlarge

Showing: 801 - 820 of 1937 results