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Plasmid#218891Purposegolden gate cloningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only
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pBDP
Plasmid#217827PurposeStuffer plasmid with golden gate compatible bidirectional promoterDepositorInsertGal1/Gal10 Bidirectional Promoter
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
(J) crtYB(K) G3 (SBE117)
Plasmid#194996PurposeRhodotorula toruloides gene phytoene synthase/lycopene cyclase (crtYB, RHTO_04605) for G3DepositorInsert(J) crtYB(K)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(G) crtI (H) G2 (SBE116)
Plasmid#194995PurposeRhodotorula toruloides gene phytoene dehydrogenase (crtI, RHTO_04602) for G2DepositorInsert(G) crtI (H)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(D) crtE (E)_G1 (SBE115)
Plasmid#194994PurposeRhodotorula toruloides gene geranylgeranyl diphosphate synthase (crtE, RHTO_02504) for G1DepositorInsert(D) crtE (E)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGA-red-mini
Plasmid#196338PurposeEasy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screeningDepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assembly backboneTagsExpressionMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGA-red-maxi
Plasmid#196337PurposeEasy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screeningDepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assembly backboneTagsExpressionMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAV10
Plasmid#63213PurposepUC19 backbone (Amp), contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. NotI or FseI sites for TU digest.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
DVK_pBR322_AF
Plasmid#217593PurposeDestination vector with kanamycin resistance and pBR322 origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR_AtU6p_BsaI_ccdb_gRNA
Plasmid#225986PurposeTo make Gateway entry clone of CRISPR guide RNA under AtU6pDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
DVK_p15A_AF
Plasmid#217583PurposeDestination vector with kanamycin resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVK_p15A_AE
Plasmid#217582PurposeDestination vector with kanamycin resistance and p15A origin of replication. Carries A-E CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVC_pBR322_AF
Plasmid#217598PurposeDestination vector with chloramphenicol resistance and pBR322 origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVC_pBR322_AE
Plasmid#217597PurposeDestination vector with chloramphenicol resistance and pBR322 origin of replication. Carries A-E CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVC_p15A_AF
Plasmid#217588PurposeDestination vector with chloramphenicol resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBD016
Plasmid#201307PurposeSr27 as Golden Gate compatible MoClo CDS partDepositorInsertSr27
UseTagsExpressionPlantMutationremoval of BsaI and BpiI sitesPromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPD1159 pLink 9
Plasmid#139247PurposeVector that holds the linker for circuits with 9 TUsDepositorInsertLinker9
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD1158 pLink 8
Plasmid#139246PurposeVector that holds the linker for circuits with 8 TUsDepositorInsertLinker8
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD1157 pLink 7
Plasmid#139245PurposeVector that holds the linker for circuits with 7 TUsDepositorInsertLinker7
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD1156 pLink 6
Plasmid#139244PurposeVector that holds the linker for circuits with 6 TUsDepositorInsertLinker6
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only