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Plasmid#133789PurposeCAG promoter expression plasmid for human codon optimized CjeCas9 nuclease with C-terminal NLS (SV40)DepositorInserthuman codon optimized CjeCas9
UseTagsNLS(SV40)-3xFLAGExpressionMammalianMutationn/aPromoterCAGAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-EGFP_opt
Plasmid#106334PurposeGateway Entry vector for EGFP (codon optimized for efficient gene synthesis)DepositorInsertEGFP
UseGateway entry vectorTagsExpressionBacterial and MammalianMutationcodon optimized for gene synthesis without affect…PromoterAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-Sestrin3
Plasmid#72592Purposeoverexpression, codon optimized for mammalian expressionDepositorInsertSESN3 (SESN3 Human)
UseTagsFLAGExpressionMammalianMutationcodon optimized for mammalian expression, see dep…PromoterCMVAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-TorA(sp)-mTurquoise2
Plasmid#111254PurposeBroad host-range bacterial expression vector with constitutive Pc promoter followed by E. coli TorA signal peptide in frame with mTurquoise2 (codon optimized for P. fluorescens); export to periplasmDepositorInsertTorA(sp)-mTurquoise2
UseTagsExpressionBacterialMutationmTurquoise2 is codon optimized for expression in …PromoterPcAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1 p37
Plasmid#169015PurposeExpresses GST-p37 (human, codon optimized for expression in bacteria)DepositorInsertp37 (UBXN2B Human)
UseTagsGSTExpressionBacterialMutationsequence was codon optimized for expression in E.…PromotertacAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hNme2Cas9-NLS(SV40)-3xFLAG (KAC582)
Plasmid#134331PurposeCAG promoter expression plasmid for human codon optimized Nme2Cas9 nuclease with C-terminal NLS (SV40) and 3x FLAG tagDepositorInserthuman codon optimized Nme2Cas9
UseTagsNLS(SV40)-3xFLAGExpressionMammalianMutationPromoterCAGAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTE4254
Plasmid#89050PurposeExpresses St chimeric gRNA and human codon-optimized StCas9DepositorInsertsSt chimeric gRNA
hStCas9
UseCRISPRTagsSV40 NLSExpressionMammalianMutationPromoterCMV and human U6Available sinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKS7107
Plasmid#89051PurposeExpresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9DepositorInsertsNm crRNA
Nm tracrRNA
hNmCas9
UseCRISPRTagsHA tag, NLS, and SV40 NLSExpressionMutationPromoterEF1a and human U6Available sinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hSt3Cas9-NLS(SV40)-3xFLAG (KAC426)
Plasmid#133788PurposeCAG promoter expression plasmid for human codon optimized St3Cas9 nuclease with C-terminal NLS (SV40)DepositorInserthuman codon optimized St3Cas9
UseTagsNLS(SV40)-3xFLAGExpressionMammalianMutationn/aPromoterCAGAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hNmeCas9-NLS(SV40)-3xFLAG (KAC409)
Plasmid#133790PurposeCAG promoter expression plasmid for human codon optimized NmeCas9 nuclease with C-terminal NLS (SV40)DepositorInserthuman codon optimized NmeCas9
UseTagsNLS(SV40)-3xFLAGExpressionMammalianMutationn/aPromoterCAGAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-IEo
Plasmid#201992PurposeAAV vector mediating inducible expression of codon optimized immediate early gene IE180 of pseudorabies virusDepositorInsertInducible expression of codon optimized immediate early gene IE180 of pseudorabies virus
UseAAVTagsExpressionMutationPromoterTight TREAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-6xHis-TEV-ORF9c
Plasmid#201023PurposeBacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site.DepositorInsertopen reading frame 9c
UseTags6xHis-TEVExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG101-Y45D
Plasmid#188105PurposeExpresses human ATG101 HORMA Y45D mutant in mammalian cellsDepositorInsertATG101 (ATG101 Human)
UseTagsGST tagExpressionMammalianMutationcodon-optimized ATG101-Y45DPromoterCMVAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ATG13 (1-197)
Plasmid#188074PurposeTo make ATG101/ATG13 HORMA complex for expression in mammalian cellsDepositorInsertATG13 (ATG13 Human)
UseTagsExpressionMammalianMutationcodon-optimized ATG13 (1-197)PromoterCMVAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA1(T16A)-NLS(SV40) (KAC511)
Plasmid#133797PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T16A) with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA1(T16A)
UseTagsNLS(SV40)ExpressionMammalianMutationT16APromoterCMV and T7Available sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA1-Leu-NLS(SV40) (KAC440)
Plasmid#133799PurposeCMV and T7 promoter expression plasmid for human codon optimized Leu-AcrIIA1 with C-terminal NLS (SV40)DepositorInserthuman codon optimized Leu-AcrIIA1
UseTagsNLS(SV40)ExpressionMammalianMutationn/aPromoterCMV and T7Available sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA1-Eriv-NLS(SV40) (KAC442)
Plasmid#133800PurposeCMV and T7 promoter expression plasmid for human codon optimized Eriv-AcrIIA1 with C-terminal NLS (SV40)DepositorInserthuman codon optimized Eriv-AcrIIA1
UseTagsNLS(SV40)ExpressionMammalianMutationn/aPromoterCMV and T7Available sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-rtTA3G-IH
Plasmid#214118PurposeGene expression by Piggybac transposase in mammalian cellsDepositorInserttrTA3G
UseTagsExpressionMammalianMutationCodon-optimized for mammalian cellsPromoterAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-rtTAM2-IB
Plasmid#214117PurposeGene expression by Piggybac transposase in mammalian cellsDepositorInsertrtTAM2
UseTagsExpressionMammalianMutationCodon-optimized for mammalian cellsPromoterAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
FRP322
Plasmid#159303PurposeYeast tagging vector to create gene fusion with yeast codon optimized CitrineA206K under KanMX selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationV2del, S3del, F64L, A206K, L231HPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only