-
Plasmid#84550PurposeHybrid reporter construct with 8 transcription factor binding sites (2 UAS, 2 TetO, 2 UAS, 2 TetO) driving EYFPDepositorInsertU2T2U2T2-EYFP
UseTagsExpressionMammalianMutationPromoterCMV-minAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2U2T2U2-YFP
Plasmid#84552PurposeHybrid reporter construct with 8 transcription factor binding sites (2 TetO, 2 UAS, 2 TetO, 2 UAS) driving EYFPDepositorInsertT2U2T2U2-EYFP
UseTagsExpressionMammalianMutationPromoterCMV-minAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Rap-MESA-20-Tev
Plasmid#84555PurposeMESA protease chain with rapamycin FKBP ectodomain, 20 extracellular linkers, and Tev proteaseDepositorInsertRap-MESA-FKBP-20-Tev
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFLinkC-AI(G3)-PDZ(GPG-G)FN3(GGS4)-TVMV
Plasmid#137117PurposeProtease Switch Clone ID: 2-A5DepositorInsertAI(G3)-PDZ(GPG-G)FN3(GGS4)-TVMV
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFLinkC-AI(GPG)-FKBP12(TP11)-FRB(GPG)-TVMV
Plasmid#137115PurposeProtease Switch Clone ID: 1-A9DepositorInsertAI(GPG)-FKBP12(TP11)-FRB(GPG)-TVMV
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFLinkC-AI(GPG)-FKBP12(EAAAK16)-FRB(PAS5)-TVMV
Plasmid#137116PurposeProtease Switch Clone ID: 1-C9DepositorInsertAI(GPG)-FKBP12(EAAAK16)-FRB(PAS5)-TVMV
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEY31CBiT
Plasmid#127761PurposeResearch the effect of gene orientation of transcriptionDepositorInsertConvergent LacI-TetR Toggle
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEYD1A
Plasmid#127759PurposeResearch the effect of gene orientation of transcriptionDepositorInsertmSpinach, MG RNA aptamer in divergent orientation
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEYRC-D1A
Plasmid#127753PurposeResearch the effect of gene orientation of transcriptionDepositorInsertRFP, CFP, Divergent Orientation
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEYT1A
Plasmid#127760PurposeResearch the effect of gene orientation of transcriptionDepositorInsertmSpinach, MG RNA aptamer in tandem orientation
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEYRC-C1A
Plasmid#127752PurposeResearch the effect of gene orientation of transcriptionDepositorInsertRFP, CFP, Convergent Orientation
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
IgH P+ VA20
Plasmid#127249PurposeIgH V-regionDepositorInsertPromoter+V-region of IgH of A20 B cell line
UseOtherTagsExpressionMutationPromoterIgHAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMJA008
Plasmid#128512PurposeExpresses Xylanase CDepositorInsertXylanase C
UseTagsExpressionBacterialMutationPromoterHistone 4BAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 Hs 1-16 3x FLAG
Plasmid#111966PurposeHuman HEATs 1-16 have been inserted into Hsh155 3xFLAG.DepositorInsertHsh155 Hs 1-16 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationHuman HEATs 1-16 insertedPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
6xmyc_cort_UAS-P
Plasmid#112991PurposeP element vector for transposon insertion of Myc-tagged Drosophila cort (Cdc20) into Drosophila genomeDepositorInsertcort (Cdc20) (cort Fly)
UseP insertion vectorTags6X myc epitopeExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 Hs 1-12 3xFLAG
Plasmid#111965PurposeHuman HEATs 1-12 have been inserted into Hsh155 3xFLAG.DepositorInsertHsh155 Hs 1-12 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationHuman HEATs 1-12 insertedPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 N4A 3xFLAG
Plasmid#111975PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker. It has 3xFLAG at the C-terminus. Contains the N4A mutation.DepositorInsertHsh155 N4A 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationT178A R181A R182A R186APromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 K818A 3xFLAG
Plasmid#111982PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains K818A mutation. LethalDepositorInsertHsh155 K818A 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationK818APromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 R775A 3xFLAG
Plasmid#111977PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains R775A mutation. LethalDepositorInsertHsh155 R775A 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationR775APromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 R778A 3xFLAG
Plasmid#111979PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains R778A mutation. LethalDepositorInsertHsh155 R778A 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationR778APromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 Hs 12-20 3xFLAG
Plasmid#111972PurposeHuman HEATs 12-20 have been inserted into Hsh155 3xFLAG.DepositorInsertHsh155 Hs 12-20 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationHuman HEATs 12-20 insertedPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 Hs 5-12 3xFLAG
Plasmid#111968PurposeHuman HEATs 5-12 have been inserted into Hsh155 3xFLAG.DepositorInsertHsh155 Hs 5-12 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationHuman HEATs 5-12 insertedPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 Hs 5-20 3xFLAG
Plasmid#111971PurposeHuman HEATs 5-20 have been inserted into Hsh155 3xFLAG.DepositorInsertHsh155 Hs 5-20 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationHuman HEATs 5-20 insertedPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 Hs 1-5 3xFLAG
Plasmid#111963PurposeHuman HEATs 1-5 have been inserted into Hsh155 3xFLAG.DepositorInsertHsh155 Hs 1-5 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationHuman HEATs 1-5 insertedPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 Hs 5-16 3xFLAG
Plasmid#111969PurposeHuman HEATs 5-16 have been inserted into Hsh155 3xFLAG.DepositorInsertHsh155 Hs 5-16 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationHuman HEATs 5-16 insertedPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHU6_Ch5_155183064-gRNA-for
Plasmid#81214PurposepHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 3' region of pCALNL_Ch5 reporterDepositorInserthU6 expression of gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHU6_Ch5_155183064-gRNA-rev
Plasmid#81215PurposepHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch5 reporterDepositorInserthU6 expression of gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHU6_gRNA_Ch10-for2
Plasmid#81209Purposefor targeting Ch10 psuedo gix site in PCDH15 gene (five base pairs from the cas9 site and 3' of gix site)DepositorInserthU6 expression of gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.30
Plasmid#73798PurposeNabi2.30 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.30
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.104
Plasmid#73799PurposeNabi2.104 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.104
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.125
Plasmid#73800PurposeNabi2.125 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.125
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.216
Plasmid#73801PurposeNabi2.216 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.216
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.226
Plasmid#73802PurposeNabi2.226 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.226
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.213
Plasmid#73757PurposeNabi2.213 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.213
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestMYC_RBPMS2
Plasmid#65668PurposeMYC expression vector with RBPMS2DepositorInsertRBPMS2 (RBPMS2 Human)
UseTagsMYCExpressionMammalianMutationintroduced HindIII site to exchange FLAG-HA tag w…PromoterCMV, 2x TetAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
Gal4pBS13-Pleum
Plasmid#60332PurposeContains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
AI-CN491
Plasmid#62594PurposeTALEN array receiving vector with half-site NN RVDDepositorTypeEmpty backboneUseTALEN; T7Tags3× FLAG and FOKI WTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
AI-CN443
Plasmid#62593PurposeTALEN array receiving vector with half-site NG RVDDepositorTypeEmpty backboneUseTALEN; T7Tags3× FLAG and FOKI WTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
AI-CN442
Plasmid#62591PurposeTALEN array receiving vector with half-site HD RVDDepositorTypeEmpty backboneUseTALEN; T7Tags3× FLAG and FOKI WTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
AI-CN441
Plasmid#62590PurposeTALEN array receiving vector with half-site NI RVDDepositorTypeEmpty backboneUseTALEN; T7Tags3× FLAG and FOKI WTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
Gal4pBS3-Pleum
Plasmid#60330PurposeContains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Gal4pBS1-Pleum
Plasmid#59993PurposeContains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Gal4pBS2-Pleum
Plasmid#59992PurposeContains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
UASGAL-CU1-Pleum
Plasmid#60331PurposeContains a promoter comprised of derivatives from native promoters galactose, cyc and leucine.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET:CACT:GFP
Plasmid#60737PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CACT' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
SNV-memTdTomato
Plasmid#53123Purposeretroviral vector expressing membrane TdTomato, driven by the Spleen Necrosis Virus promoter; for use in chicken cellsDepositorInsertmemTdTomato
UseRetroviral; Expression in chicken cellsTagsExpressionMutationPromoterSNV (spleen necrosis virus)Available sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
SNV-lacZ
Plasmid#53125Purposeretroviral vector expressing lacZ, driven by the Spleen Necrosis Virus promoter; for use in chicken cellsDepositorInsertlacZ (lacZ E.coli)
UseRetroviral; Expression in chicken cellsTagsExpressionMutationPromoterSNV (spleen necrosis virus)Available sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MAVS-M1A500(KaganF60)
Plasmid#52003PurposeExpresses the human MAVS CDS containing a point mutation M1A and a C-terminal deletion removing the transmembrane domainDepositorInsertMAVS-M1A-500
UseTagsExpressionMammalianMutationchanged Met 1 to Ala and deleted the C-terminal t…PromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-5'UTR-MAVS-ORF1(KaganE49)
Plasmid#52006PurposeExpresses the human MAVS CDS and contains the 5'UTR sequence with a mutation in the start codon 37 nt upstream of the FL MAVS start codonDepositorInsert5utr-MAVS-ORF1mut
UseTagsExpressionMammalianMutationmutation removing the start codon for ORF1PromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MAVS-M1A,M142A-invitro(KaganE14)
Plasmid#52018PurposeUsed for in vitro expression of the human MAVS CDS containing the point mutations M1A,M142ADepositorInsertMAVS-M1A,M142A
UseTagsExpressionMammalianMutationchanged Met 1 to Ala and Met 142 to AlaPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only