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Plasmid#106405PurposeFor expressing Trpa1b-2A-EGFP in zebrafish cardiomyocytes.DepositorInsertsUseGatewayTags2A-EGFPExpressionMutationPromoterAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only
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EGFR∆550-580-mEGFP
Plasmid#203788PurposeNumber and brightness analysis of EGFR oligomerizationDepositorInsertEGFR (EGFR Human)
UseTagsmEGFPExpressionMammalianMutationaa 550-580 deletedPromoterAvailable sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-HMGB1-delIDR
Plasmid#194554PurposeMammalian Expression of mEGFP-HMGB1 WT Fusion Protein, "del IDR" variantDepositorInsertHMGB1 (HMGB1 Human)
UseTagsmEGFPExpressionMammalianMutationtruncation by stop codon after Asn134PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
p5189 pSUPER-shRNA-GFP
Plasmid#24747PurposeAddgene QC next-generation sequencing identified retroviral plasmid backbone elements.DepositorInsertshRNA-GFP
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJune 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLVXPuro-epsilon-COP-GFP
Plasmid#66604PurposeExpresses epsilon COP as a GFP fusionDepositorInsertepsilon COP (COPE Bovine)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFCK(1.3)GFP-SynaptophysW
Plasmid#27235DepositorInsertEGFP
UseLentiviralTagsSynaptophys-GFPExpressionMutationPromoterAvailable sinceMarch 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pT2Apuro-Stargazin-mEGFP-iLID
Plasmid#178529PurposeExpresses Stargazin-mEGFP-iLID in mammalian cellsDepositorInsertStargazin-mEGFP-iLID
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6.2-GW/EmGFP-miR-SUMO23
Plasmid#31073DepositorUseRNAiTagsEmGFPExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt2(K14A/R25E)
Plasmid#86595PurposeExpresses eGFP-tagged human Akt2(K14A/R25E) membrane-binding mutantDepositorInsertAkt2(K14A/R25E) (AKT2 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTub-alpha1-Lifeact-GFP
Plasmid#175437PurposeF-actin structures labellingDepositorInsertLifeact-EGFP
UseTagsEGFPExpressionMammalianMutationPromoterpTub-alpha1Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTol-Promoter:NLS-tdMCP-eGFP
Plasmid#86248PurposeFor generating transgenic lines that ubiquitously expressing the fluorescent MCP reporterDepositorInsertbeta Actin promoter:NLS-tdMCPeGFP
UseUsed with transposase to make transient or stable…TagsNLS-tdMCP-eGFPExpressionMutationPromoterBeta ActinAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19-URA3-3'SEC13-GFP
Plasmid#25445DepositorInsertSec13-Green Fluorescent Protein
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-TetR-NLS-VP16
Plasmid#103834PurposeTranscription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arraysDepositorInsertEGFP-TetR-NLS-VP16 (tetR )
UseTagsExpressionMammalianMutationPromoterSV2Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-TagRFP-gcry-eGFP
Plasmid#117767PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_GST-TEV-EGFP-TBK1
Plasmid#187830PurposePlasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631DepositorInsertTBK1 (TBK1 Human)
UseTagsGST and TEV-mEGFP-GG LinkerExpressionBacterial and InsectMutationPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ GFP PODXL FKBP 4K>R
Plasmid#202421PurposeExpression of ubiquitination-deficient GFP-tagged PODXL with FKBP-rapamycin binding (FRB) dimerization domainDepositorInsertPODXL (PODXL Human)
UseLentiviralTagsFKBP and GFPExpressionMutationK431/525/526/547RPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-RIαΔ(D/D+linker)-EGFP
Plasmid#181843PurposeGFP-tagged PKA RIα subunit lacking the docking/dimerization (D/D) and linker regions; for mammalian expression.DepositorInsertRIα-(D/D+linker)EGFP (PRKAR1A Human)
UseTagsEGFPExpressionMammalianMutationDeletion of residues 12-112 in PKA RIα.PromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW-eGFP-FHA-SHLD2-m1
Plasmid#114122PurposeLentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2, m1 mutant; 53BP1-independent recruitment to damaged chromatinDepositorInsertSHLD2 (m1 mutant) (SHLD2 Human)
UseLentiviral; Doxycycline inducibleTagsRNF8 FHA domain (aa 2-160) and eGFPExpressionMammalianMutationW489A, F494A, W495A mutations introducedPromoterTRE promoter, Tet ONAvailable sinceAug. 28, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXJ40-GFP-iBox-PAK4cat
Plasmid#190179Purposeexpresses GFP-tagged iBox-PAK4catDepositorInsertGFP-iBox-PAK4cat
UseTagsFlag and GFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP-CREB (S133A)
Plasmid#137002PurposemEGFP-CREB donor for G-CREB with S133A mutationDepositorInsertCREB1 (Creb1 Rat)
UseTagsmEGFPExpressionMammalianMutationChanges Serine 133 into AlaninePromoterCMVAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only