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Tol2-cmlc2-Trpa1b-2A-EGFP

Plasmid

#106405

Purpose

For expressing Trpa1b-2A-EGFP in zebrafish cardiomyocytes.

Depositor

Randall Peterson

Article

Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

Inserts

cmlc2 promoter
Trpa1b (trpa1b Zebrafish)

Use

Gateway

Tags

2A-EGFP

Expression

Mutation

Promoter

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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EGFR∆550-580-mEGFP

Plasmid

#203788

Purpose

Number and brightness analysis of EGFR oligomerization

Depositor

Thorsten Wohland

Article

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

EGFR (EGFR Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

aa 550-580 deleted

Promoter

Available since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-delIDR

Plasmid

#194554

Purpose

Mammalian Expression of mEGFP-HMGB1 WT Fusion Protein, "del IDR" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

truncation by stop codon after Asn134

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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p5189 pSUPER-shRNA-GFP

Plasmid

#24747

Purpose

Addgene QC next-generation sequencing identified retroviral plasmid backbone elements.

Depositor

Peter Howley

Article

Schweiger et al J Virol. 2006 May . 80(9):4276-85.

Insert

shRNA-GFP

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

June 2, 2010

Availability

Academic Institutions and Nonprofits only

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pLVXPuro-epsilon-COP-GFP

Plasmid

#66604

Purpose

Expresses epsilon COP as a GFP fusion

Depositor

David Stephens

Article

Morohashi et al Biochem J. 2010 Apr 14;427(3):401-12. do

Insert

epsilon COP (COPE Bovine)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pFCK(1.3)GFP-SynaptophysW

Plasmid

#27235

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

Synaptophys-GFP

Expression

Mutation

Promoter

Available since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pT2Apuro-Stargazin-mEGFP-iLID

Plasmid

#178529

Purpose

Expresses Stargazin-mEGFP-iLID in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

Stargazin-mEGFP-iLID

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA6.2-GW/EmGFP-miR-SUMO23

Plasmid

#31073

Depositor

Wulf Paschen

Article

Yang et al Biochem Biophys Res Commun. 2009 Apr 24.

Insert

SUMO2/3 microRNA (SUMO2, SUMO3 Human)

Use

RNAi

Tags

EmGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt2(K14A/R25E)

Plasmid

#86595

Purpose

Expresses eGFP-tagged human Akt2(K14A/R25E) membrane-binding mutant

Depositor

Ivan Yudushkin, Thomas Leonard

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2(K14A/R25E) (AKT2 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pTub-alpha1-Lifeact-GFP

Plasmid

#175437

Purpose

F-actin structures labelling

Depositor

Frank Bradke, Sebastián Dupraz

Article

Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

Insert

Lifeact-EGFP

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

pTub-alpha1

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pTol-Promoter:NLS-tdMCP-eGFP

Plasmid

#86248

Purpose

For generating transgenic lines that ubiquitously expressing the fluorescent MCP reporter

Depositor

Florence Marlow

Article

Campbell et al Development. 2015 Apr 1;142(7):1368-74.

Insert

beta Actin promoter:NLS-tdMCPeGFP

Use

Used with transposase to make transient or stable…

Tags

NLS-tdMCP-eGFP

Expression

Mutation

Promoter

Beta Actin

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pUC19-URA3-3'SEC13-GFP

Plasmid

#25445

Depositor

Benjamin Glick

Article

Rossanese et al J Cell Biol. 1999 Apr 5. 145(1):69-81.

Insert

Sec13-Green Fluorescent Protein

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP-TetR-NLS-VP16

Plasmid

#103834

Purpose

Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

EGFP-TetR-NLS-VP16 (tetR )

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV2

Available since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pPRISM-TagRFP-gcry-eGFP

Plasmid

#117767

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac_Dual_GST-TEV-EGFP-TBK1

Plasmid

#187830

Purpose

Plasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Use

Tags

GST and TEV-mEGFP-GG Linker

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pQCXIZ GFP PODXL FKBP 4K>R

Plasmid

#202421

Purpose

Expression of ubiquitination-deficient GFP-tagged PODXL with FKBP-rapamycin binding (FRB) dimerization domain

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

PODXL (PODXL Human)

Use

Lentiviral

Tags

FKBP and GFP

Expression

Mutation

K431/525/526/547R

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-RIαΔ(D/D+linker)-EGFP

Plasmid

#181843

Purpose

GFP-tagged PKA RIα subunit lacking the docking/dimerization (D/D) and linker regions; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

RIα-(D/D+linker)EGFP (PRKAR1A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of residues 12-112 in PKA RIα.

Promoter

CMV

Available since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCW-eGFP-FHA-SHLD2-m1

Plasmid

#114122

Purpose

Lentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2, m1 mutant; 53BP1-independent recruitment to damaged chromatin

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD2 (m1 mutant) (SHLD2 Human)

Use

Lentiviral; Doxycycline inducible

Tags

RNF8 FHA domain (aa 2-160) and eGFP

Expression

Mammalian

Mutation

W489A, F494A, W495A mutations introduced

Promoter

TRE promoter, Tet ON

Available since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pXJ40-GFP-iBox-PAK4cat

Plasmid

#190179

Purpose

expresses GFP-tagged iBox-PAK4cat

Depositor

Bianxiao Cui

Article

Li et al Nano Lett. 2019 Oct 9;19(10):6955-6963.

Insert

GFP-iBox-PAK4cat

Use

Tags

Flag and GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP-CREB (S133A)

Plasmid

#137002

Purpose

mEGFP-CREB donor for G-CREB with S133A mutation

Depositor

Ryohei Yasuda

Article

Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

Insert

CREB1 (Creb1 Rat)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Changes Serine 133 into Alanine

Promoter

CMV

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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