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pUCBB-ntH6-eGFP

Plasmid

#32557

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

6 His tag with Thrombin site

Expression

Bacterial

Mutation

Promoter

Consitutive lac

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pYFAC

Plasmid

#168982

Purpose

Filamentous fungi vector. Also referred to as pYFAC-pyrG.

Depositor

Yit Heng Chooi

Article

Chooi et al Environ Microbiol. 2017 May;19(5):1975-1

Type

Empty backbone

Use

Synthetic Biology; Heterologous expression in fil…

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR L1-vas2-Cre-L4

Plasmid

#62301

Purpose

Cre recombinase-expressing helper plasmid for Anopheles gene engineering, vasa promoter

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Insert

Cre recombinase under control of vasa promoter and terminator

Use

Tags

HA and SV40 NLS

Expression

Insect

Mutation

Promoter

vasa

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pMOD_A0508

Plasmid

#91014

Purpose

Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

EC1.2

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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WFS1 P724L-Flag-pcDNA3

Plasmid

#13012

Depositor

Fumihiko Urano

Article

Fonseca et al J Biol Chem. 2005 Nov 25. 280(47):39609-

Insert

Wolfram Syndrome 1 (WFS1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed P 724 to L

Promoter

Available since

Sept. 26, 2006

Availability

Academic Institutions and Nonprofits only
pCAG-Gdf9

Plasmid

#163599

Purpose

Expresses GDF-9 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Gdf9 (Gdf9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pKMV-VioC

Plasmid

#65349

Purpose

systhesis of vioC gene in the violacein pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

vioC

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pTPI1-EMC1-GFP-APEX2-URA3

Plasmid

#184776

Purpose

Expresses EMC1-GFP-APEX2 chimera for labeling yeast endoplasmic reticulum (ER). Uses auxotrophic marker URA3(Kluyveromyces lactis).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

APEX2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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NdeI – SpeI(NheI) – BamHI/BglII - ANXA2 (29-339)

Plasmid

#136544

Purpose

Expresses ANXA2 chimeras in bacterial cells

Depositor

Laszlo Nyitray

Article

Ecsedi et al Structure. 2020 Aug 4;28(8):943-953.e4.

Type

Empty backbone

Use

Tags

ANXA2 (29-339) and His-tag

Expression

Bacterial

Mutation

Promoter

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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Twist-siRNA5

Plasmid

#8456

Depositor

Bob Weinberg

Article

Yang et al Cell 2004 Jun 25;117(7):927-39.

Insert

siTwist (Twist1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
Nup54 no FG-mEGFP494(0)

Plasmid

#164269

Purpose

Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 5 amino acids at the carboxyl end of mEGFP

Depositor

Sanford Simon

Article

Pulupa et al Elife. 2020 Dec 21;9. pii: 60654. doi: 1

Insert

Nup54 (NUP54 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

deleted the FG region of Nup54

Promoter

CMV

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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P303-FBP_2

Plasmid

#139693

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPH

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

various

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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MPC020: CamKII CMV_NovArch_citrine

Plasmid

#153193

Purpose

Encodes Novarch

Depositor

Adam Cohen

Article

Chien et al Sci Adv. 2021 May 5;7(19). pii: 7/19/eab

Insert

NovArch

Use

Tags

citrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TAT-1-delta2-26-FLAG

Plasmid

#35983

Depositor

Susana Valente

Article

Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

Insert

transactivating protein (tat )

Use

Tags

Flag

Expression

Mammalian

Mutation

tat from HIV1 aa 2-26 deleted

Promoter

Available since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only

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Dendra2-Gamma-Tubulin-17

Plasmid

#57721

Purpose

Localization: Centrosomes, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Gamma-Tubulin (TUBG1 Human)

Use

Tags

Dendra2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-Tag2B BimEL (3SA)

Plasmid

#23091

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

Bim EL (3SA) (Bcl2l11 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed Serine 55/65/73 to alanine

Promoter

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only
pMOD_A1111

Plasmid

#91032

Purpose

Module A, Promoter: OsAct1, Gene: TaCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

OsAct1

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMC-Ura-Cen-lvl1

Plasmid

#176182

Purpose

preassembled yeast MoClo level-1 backbone with GFP dropout and URA3 marker

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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PB-Mycn

Plasmid

#60667

Purpose

PiggyBac expression vector encoding murine Mycn downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Mycn (Mycn Mouse)

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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HisMBP CFlm25

Plasmid

#78458

Purpose

Inducible expression in E. coli

Depositor

Sylvie Doublie

Article

Coseno et al Nucleic Acids Res. 2008 Jun;36(10):3474-

Insert

Human Cleavage Factor lm 25 kDa subunit (NUDT21 Human)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only