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Plasmid#129691Purposeexpress E. coli GreB E82C/C68S/D41NDepositorInsertGreB E82C C68S D41N (greB E. coli)
UseTagshis6ExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
miR-722 plix4.03
Plasmid#97140PurposeLentiviral tet-inducible expression of miR-722and Dendra 2DepositorInsertmiR-722 (mir722 Zebrafish)
UseLentiviralTagsdendra2ExpressionMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15b-GBdm
Plasmid#129690Purposeexpress E. coli GreB E82C/C68SDepositorInsertGreB E82C C68S (greB E. coli)
UseTagshis6ExpressionBacterialMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB153 - pL2_pSB90_2x35S::ZCT1::tMAS
Plasmid#123195Purposebinary plant vector for transient expression of ZCT1 from Catharanthus roseusDepositorInsert2x35S::ZCT1::tMAS
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB160 - pL2_pSB90_2x35S::ORCA3::tMAS
Plasmid#123196Purposebinary plant vector for transient expression of ORCA3 from Catharanthus roseusDepositorInsert2x35S::ORCA3::tMAS
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB166 - pL2_pSB90_tMAS::rLUC-I::pMAS
Plasmid#123199Purposebinary plant vector for transient expression of a Renilla luciferase (rLUC, with intron)DepositorInserttMAS::rLUC-I::pMAS
UseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1371-sgYAP-10
Plasmid#121424PurposesgYAP-10 sequence: GTGCTGTCCCAGATGAACGTC. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.DepositorInsertsgYAP-10 (GTGCTGTCCCAGATGAACGTC)
UseCRISPR and LentiviralTagsmCherryExpressionMammalianMutationPromotermouse U6Available sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluSnFR E25R
Plasmid#119831PurposeProduction of recombinant iGluSnFR E25R variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluSnFR E25R
UseTagsGSTExpressionBacterialMutationE25RPromoterT7Available sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSW615 - pATHB-8
Plasmid#115990Purposeentry vector for GreenGate cloning method, promoterDepositorInsertpATHB-8
UseUnspecifiedTagsExpressionMutationA1287T to abolish Eco31I sitePromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSD_SpCas9MT2-NLS-3xHA-NLS-SaCas9WT-2xNLS
Plasmid#107321PurposeExpresses SpCas9 (R1333S) fused to SaCas9 in mammalian cellsDepositorInsertSpCas9-SaCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 (R1333S) is fused to SaCas9PromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a - Ec-ClpX D144L
Plasmid#111514PurposeExpresses E. coli ClpX with a C-terminal His tag and a mutation from D144 to L144DepositorInsertClpX
UseTagsHis tagExpressionBacterialMutationAspartatic acid 144 to LeucinePromoterT7Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-D27S/Y100F
Plasmid#110827PurposeBacterial expression of E.coli DHFR D27S/Y100F mutantDepositorInsertecDHFR
UseTagsExpressionBacterialMutationD27 mutated to S27, Y100 mutated to F100PromoterT7Available sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
MDH1-PGK-FLAG-TAT-86-D
Plasmid#35976DepositorInserttransactivating protein (tat Human Immunodeficiency Virus)
UseRNAi and RetroviralTagsFlagExpressionMammalianMutationFrom the "D" HIV cladePromoterAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
MDH1-PGK-FLAG-TAT-86-C
Plasmid#35982DepositorInserttransactivating protein (tat Human Immunodeficiency Virus)
UseRNAi and RetroviralTagsFlagExpressionMammalianMutationFrom the "C" HIV cladePromoterAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMflCT-o4
Plasmid#101313PurposeContains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and cat resistance genes, origin of transfer of RP4 plasmid.DepositorInsertsoriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
cat resistance gene
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMflT-o1
Plasmid#101309PurposeContains: oriC1 fragment of M. florum L1 replication origin (rpmH/dnaA intergenic region), colE1 replication origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.DepositorInsertoriC1 of M. florum strain L1 (rpmH/dnaA intergenic region)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pENTR CMVie-Lifeact-mRuby2 L1-R5
Plasmid#84389PurposeMultisite Gateway entry vector for expression of mRuby2-tagged Lifeact in cells including neuronsDepositorInsertLifeact
UseGatewayTagsmRuby2ExpressionMammalianMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Zeo-NKX2-8
Plasmid#75090Purposeretrovirus-mediated gene transfer into mammalian cellsDepositorInsertNKX2-8 (NKX2-8 Human)
UseRetroviralTagsnoneExpressionMammalianMutationwild-type, full-length, without native 3' UTRPromoterAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only