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Plasmid#221312PurposeExpression of TUBA (pLxIS) in mammalian cells by retroviral transductionDepositorInsertTUBA (pLxIS) (Dnmbp Mouse)
UseRetroviralTagsFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-JCAD
Plasmid#221292PurposeExpression of JCAD (pLxIS) in mammalian cells by retroviral transductionDepositorInsertJCAD (pLxIS) (Jcad Mouse)
UseRetroviralTagsFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-KIF5C
Plasmid#221293PurposeExpression of KIF5C (pLxIS) in mammalian cells by retroviral transductionDepositorInsertKIF5C (pLxIS) (Kif5c Mouse)
UseRetroviralTagsFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-Myoglobin(pLxIS-PLPLR)
Plasmid#221285PurposeExpression of Myoglobin (pLxIS-PLPLR) in mammalian cells by retroviral transductionDepositorInsertMyoglobin (pLxIS-PLPLR) (Mb Synthetic)
UseRetroviralTags3xFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-GMIP(trunc C1)
Plasmid#221279PurposeExpression of GMIP C-terminal truncation 1 (1-64) in mammalian cells by retroviral transductionDepositorInsertGMIP C-terminal truncation 1 (1-64) (Gmip Mouse)
UseRetroviralTags3xFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-GMIP(trunc C2)
Plasmid#221280PurposeExpression of GMIP C-terminal truncation 2 (1-44) in mammalian cells by retroviral transductionDepositorInsertGMIP C-terminal truncation 2 (1-44) (Gmip Mouse)
UseRetroviralTags3xFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ChmF
Plasmid#221345PurposeExpress ChmF in E. coliDepositorInsertchmF
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ChmK
Plasmid#221346PurposeExpress ChmK in E. coliDepositorInsertchmK
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ChmL
Plasmid#221347PurposeExpress ChmL in E. coliDepositorInsertchmL
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1-chmI
Plasmid#221341PurposeExpress ChmI in E. coliDepositorInsertchmI
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ChmJ
Plasmid#221342PurposeExpress ChmJ in E. coliDepositorInsertchmJ
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNoRBS
Plasmid#218167PurposepET31b plasmid with short insert lacking ribosome binding site to increase 2',3'-cNMP levelsDepositorInsertShort insert
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pH2B-6AA-ffDronpa
Plasmid#214606PurposeExpresses reversibly switchable fluorescent protein ffDronpa targeted to the nucleusDepositorArticleInsertffDronpa-H2B
UseTagsH2B targetting with a linker of 6AAExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pvimentin-ffDronpaF
Plasmid#214607PurposeExpresses reversibly switchable fluorescent protein ffDronpaF targeted to vimentinDepositorArticleInsertffDronpaF-vimentin
UseTagstarget to vimentin (human)ExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pffDronpa2F-peroxisome
Plasmid#214608PurposeExpresses reversibly switchable fluorescent protein ffDronpa2F targeted to peroxisomeDepositorArticleInsertffDronpa2F-peroxisome
UseTagsperoxisome targeting sequence (PTS)ExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACS125
Plasmid#204593PurposeGST-lamin A C522A (389-646)DepositorInserthuman lamin A C522A (389-646)
UseTagsGST - lamin A C522A (389-646)ExpressionBacterialMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACS38
Plasmid#204590PurposeGST-Progerin(506-664 delta 50)DepositorInserthuman progerin (506-664 delta 50)
UseTagsGST - progerin (506-664 delta 50)ExpressionBacterialMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-eIF4E-TwStrep
Plasmid#208638Purposefor E. coli expression of C-ter Twin Strep tagged human eIF4E (ENST00000450253)DepositorInserthuman eIF4E (ENST00000450253) (EIF4E Human)
UseTags6xHis and Twin StrepExpressionBacterialMutationPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-mEGFP(A206K)-TwStrep
Plasmid#208622Purposefor E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) cloneDepositorInsertmEGFP(A206K)
UseTags6xHis and Twin StrepExpressionBacterialMutation(compared with pEGFP-C1, protein level) A206KPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YDHFR_R127ATS
Plasmid#191312PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationF31Y DHFR/R127A TYMSPromoterT7 (folA), Tet (thyA)Available sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only