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Plasmid#183089Purpose2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in quad-cistronic construct assemblyDepositorInsertP2A
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
promoter-less mRFP1_Violet
Plasmid#183139PurposeBioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_VioletDepositorInsertRBS, mRFP1E_Violet
UseSynthetic BiologyTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
sCatch-GFP-6xHIS
Plasmid#186904PurposeBacterial expression of soluble protein sfGFP-SpyCatcher-sfCherry1-10 with a 6xHIS tagDepositorInsertsfGFP-SpyCatcher003-sfCherry1-10-6xHIS
UseTags6xHis and sfGFPExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
sTag-BFP-6xHIS
Plasmid#186905PurposeBacterial expression of soluble protein tagBFP-sfCherry11-SpyTag with a 6xHIS tagDepositorInsertSpyTag003-sfCherry11-tagBFP-6xHIS
UseTagstagBFP, 6xHisExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET51b_SNAP-ecDHFR
Plasmid#187108PurposeExpression of SNAP-ecDHFR fusion in bacteriaDepositorInsertSNAP-ecDHFR
UseTagsHisx10 and StrepExpressionBacterialMutationPromoterT7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET51b_SNAP-ecDHFRmt
Plasmid#187109PurposeExpression of SNAP-ecDHFRmt fusion in bacteriaDepositorInsertSNAP-ecDHFRmt
UseTagsHisx10 and StrepExpressionBacterialMutationecDHFR-R44L-H45QPromoterT7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEcCas5
Plasmid#170090Purposeexpresses E. coli type I-E Cas5DepositorInsertE. coli type I-E Cas5
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEcCas7
Plasmid#170092Purposeexpresses E. coli type I-E Cas7DepositorInsertE. coli type I-E Cas7
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEcCas8
Plasmid#170093Purposeexpresses E. coli type I-E Cas8DepositorInsertE. coli type I-E Cas8
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEcCse2
Plasmid#170094Purposeexpresses E. coli type I-E Cse2DepositorInsertE. coli type I-E Cse2
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRMCD30
Plasmid#173033PurposepQE30 with S. flexneri 2a wzzBSF geneDepositorInsertS. flexneri 2a wzzBSF gene
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK29-wzzO139
Plasmid#172970PurposepWSK29 with Vibrio cholerae wzzO139 geneDepositorInsertwzz gene from Vibrio cholerae O139
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM62636
Plasmid#169095PurposeLow copy MoClo level P binary vector for plant transformationDepositorTypeEmpty backboneUseMoclo cloning vectorTagsExpressionPlantMutationPromoterAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEG01
Plasmid#154136PurposeThis plasmid carries fucO and aldA under the control of P_BAD promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.DepositorInsertfucO, aldA
UseTagsExpressionBacterialMutationPromoterP_BADAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEG02
Plasmid#154137PurposeThis plasmid carries fucO and aldA under the control of P_thrC3 promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.DepositorInsertfucO, aldA
UseTagsExpressionBacterialMutationPromoterP_thrC3Available sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHCKAn-yibDp-Q-GRFT
Plasmid#158748Purposelow phosphate yibDp induction of Q-GriffithsinDepositorInsertyibDp Q-Griffithsin
UseTagsExpressionBacterialMutationM78QPromoteryibDAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHCKAn-phoBp-GRFT
Plasmid#158746Purposelow phosphate phoBp induction of GriffithsinDepositorInsertphoBp Griffithsin
UseTagsExpressionBacterialMutationPromoterphoBAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCL27-decoder
Plasmid#159449PurposeInsert: Pfus1tet-citrine-tCyc1. The fluorescent protein Citrine is inducible by alpha-factor and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.DepositorInsertPfus1tet-citrine-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPfus1tetAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG502-MAP4-frFAST
Plasmid#160058PurposeExpresses MAP4-frFAST in mammalian cellsDepositorInsertMAP4-frFAST
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUI-SynP-CAS1sg
Plasmid#154341PurposeExpression of the sgRNA targeting to the E. coli can geneDepositorInsertCAS1 sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only