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YFP-mGCN5-C1

Plasmid

#134573

Purpose

Expresses YFP-GCN5 fusion protein

Depositor

Susan Janicki

Article

Rafalska-Metcalf et al PLoS One. 2010 Apr 21;5(4):e10272. doi:

Insert

GCN5 (Kat2a Mouse)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pTagBFP-C1-FKBP-PIP5K1C-HD-EE

Plasmid

#220090

Purpose

Recruitable PIP5K to make PIP2

Depositor

Gerry Hammond

Article

Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

Insert

PIP5K1C (PIP5K1C Human)

Use

Tags

TagBFP-FKBP1A(3-108)

Expression

Mammalian

Mutation

amino acids 1-635; D101R, R304D, R445E, K446E

Promoter

CMV

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_rtTA-P2A-BFP_HygroR

Plasmid

#186738

Purpose

rtTA-P2A-BFP expression construct cloned into lentiviral backbone

Depositor

Richard Sherwood

Article

Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

Insert

rtTA

Use

Lentiviral

Tags

P2A-BFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PI4KAC1001(D1957A)

Plasmid

#139312

Purpose

Recruitable PI4KA kinase-dead catalytic fragment

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

mCherry: FKBP1A(3-108): GGSA]4GG:PI4KA(1102-2103) (PI4KA Human)

Use

Tags

Expression

Mammalian

Mutation

D1957A, G1986D

Promoter

Available since

April 8, 2020

Availability

Academic Institutions and Nonprofits only

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pFS16 Pmyo-3 NPP-9 mChBioFLAG

Plasmid

#45099

Depositor

Steven Henikoff

Article

Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

Inserts

npp-9 (npp-9 Nematode)
unc-119 (unc-119 Nematode)

Use

Tags

BLRP, FLAG, and mCherry

Expression

Worm

Mutation

Promoter

myo-3 and unc-119

Available since

June 6, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP C1 SADBs

Plasmid

#67154

Purpose

pEGFP containing N-terminally EGFP Tagged mouse SADBs

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

Brsk1 (Brsk1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-Ef1a-DO-hChR2(H134R)-mCherry-WPRE-pA

Plasmid

#37082

Purpose

Cre-dependent expression of hChR2(H134R) for optogenetic excitation

Depositor

Bernardo Sabatini

Article

Kozorovitskiy et al Nature. 2012 May 13;485(7400):646-50. do

Insert

hChr2(H134R)-mCherry

Use

AAV and Cre/Lox; Cre-off

Tags

mCherry

Expression

Mutation

Promoter

EF1a

Available since

June 14, 2012

Availability

Academic Institutions and Nonprofits only

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pCIP-Flag-TfebAA

Plasmid

#79014

Purpose

For mammalian expression of a Flag-tagged, constitutively active version (S142A, S211A) of Tfeb.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

Tfeb (Tfeb Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

S142A, S211A

Promoter

pCAGGs

Available since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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pDIRECT_23A

Plasmid

#91138

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:bar

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:AtCas9 + AtU6:gRNA

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pTC364

Plasmid

#91213

Purpose

protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, Csy4 processing)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

2 gRNAs targeting tomato ARF8A

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pTC394

Plasmid

#91224

Purpose

protoplast vector expressing gRNA24 targeting tomato ANT1 (control without TREX2 expression)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNA targeting tomato ANT1

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pTC392

Plasmid

#91223

Purpose

protoplast vector expressing gRNA23 targeting tomato ANT1 (control without TREX2 expression)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNA targeting tomato ANT1

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pSLIK TT RBFOX2

Plasmid

#59770

Purpose

doxycycline-inducible expression of untagged human RBFOX2 in mammalian cells

Depositor

Phil Sharp

Article

Jangi et al Genes Dev. 2014 Mar 15;28(6):637-51. doi

Insert

RBFOX2 (RBFOX2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE tight

Available since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pDULE-ABK

Plasmid

#49086

Purpose

A pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experiments

Depositor

Peter Schultz

Article

Ai et al Chembiochem. 2011 Aug 16;12(12):1854-7.

Inserts

tRNA synthetase
tRNA for a photocrosslinking amino acid

Use

Tags

Expression

Bacterial

Mutation

L274M, C313A, Y349F

Promoter

GlnRS and lpp

Available since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pCW57.1-4EBP1_4xAla

Plasmid

#38240

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

4EBP1 (Eif4ebp1 hybrid gene of rat and human, Rat)

Use

Lentiviral; Doxycycline inducible

Tags

Expression

Mammalian

Mutation

T37A, T46A, S65A, T70A (dominant negative)

Promoter

Tet ON

Available since

Nov. 15, 2012

Availability

Academic Institutions and Nonprofits only

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ECFP-ER-0

Plasmid

#55342

Purpose

Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

ER (CALR Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pmCherry-ATG5

Plasmid

#13095

Depositor

Roberta Gottlieb

Article

Hamacher-Brady et al Cell Death Differ. 2006 Apr 28. ():.

Insert

ATG5 (Atg5 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2006

Availability

Academic Institutions and Nonprofits only

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Cas9 MDC123

Plasmid

#59184

Purpose

2x35S promoting Glycing Max codon optimized Cas9 with bar plant selection

Depositor

Robert Stupar

Article

Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

Insert

Glycine Max codon optimized Cas9

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

2X35S

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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SG3ΔENV RT D185A

Plasmid

#145782

Purpose

Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and D185A mutation in reverse transcriptase (RT D185A).

Depositor

Wesley Sundquist

Article

Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

Insert

Reverse Transcriptase

Use

Lentiviral and Retroviral

Tags

Expression

Mammalian

Mutation

Changed Reverse Transcriptase Aspartic Acid 185 t…

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pL0-MtBCP1Pro

Plasmid

#159415

Purpose

Medicago truncatula BCP1 promoter sequence (1108 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

Depositor

Sam Brockington

Article

Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

Insert

MtBCP1 Promoter

Use

Puc19-derived

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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