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pUC19-TgKI-MCS-p2A-tdTomato-polyA

Plasmid

#174027

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

p2A-tdTomato

Use

Zebrafish knock-in tagging

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CLYBL-Bxb1-LP-v2-TC

Plasmid

#194327

Purpose

CLYBL targeting construct with the Bxb1 landing pad version 2 cassette.

Depositor

Richard Davis

Article

Blanch-Asensio et al Stem Cell Res. 2023 Feb;66:102991. doi:

Insert

loxP-EBFP2-attP-BleoR-lox251

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsTNRC6A

Plasmid

#25035

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

TNRC6A (TNRC6A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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pPAGFP-VSVG

Plasmid

#11915

Depositor

Jennifer Lippincott-Schwartz

Article

Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

Insert

VSVG

Use

Tags

Expression

Mammalian

Mutation

(L64F/T65S/V163A/T203H). The PAGFP also contains …

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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NmCas9_sgRNA_expression_in_pBluescript

Plasmid

#122091

Purpose

U6 driven NmCas9 sgRNA expression vector for cloning own guides

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

NmCas9 tracrRNA (NEWENTRY Synthetic, S. pyogenes)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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NLS-AgDD

Plasmid

#80625

Purpose

create nuclear protein aggregates in mammalian cells

Depositor

Thomas Wandless

Article

Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

Insert

NLS-NES-L106P-sfGFP (FKBP1A Human)

Use

Tags

MPPKKKRKVLALKLAGLDI

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pLX304 CMV OMM-FKBP-V5-sTurboID (N)

Plasmid

#153006

Purpose

Expresses split-TurboID N-terminal fragment targeted to outer mitochondrial membrane

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

OMM (Tom20)-sTurboID (N) (L73)

Use

Lentiviral

Tags

FKBP and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ131B

Plasmid

#69281

Purpose

Golden Gate entry vector; 1st gRNA under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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5KO9 (HMCES)

Plasmid

#101877

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

HMCES (HMCES Human)

Use

Tags

6x His (modified C-term tag is AENLYFQSHHHHHH)

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 30, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pSBbi 41BBL CD86 HLA-A201

Plasmid

#183248

Purpose

Expression of human 41BBL, CD86 and HLA-A201 in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

Depositor

Alexander McLellan

Article

Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

Inserts

CD86, 41BBL
HLA-A2 (HLA-A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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