-
Plasmid#177076PurposeMoClo Level 1, position 2, transcriptional unit for transient expression of major latex protein-like (NmMLPL) from Nepeta mussinii promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31DepositorInsertmajor latex protein-like (NmMLPL) from Nepeta mussinii
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0120
Plasmid#177052PurposeMoClo Level 1, position 1, transcriptional unit for transient expression of major latex protein-like (NmMLPL) from Nepeta mussinii driven by 35S promoter; contains a chloroplast transit peptideDepositorInsertmajor latex protein-like (NmMLPL) from Nepeta mussinii
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGlomyc-Ovol1
Plasmid#172871PurposeMammalian expression vector with myc-tagged mouse Ovol1 coding sequenceDepositorInsertOvol1 (Ovol1 Mouse)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.U6gT28.13.hSyn.H2B.RFP
Plasmid#170372PurposePlasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Expressing nuclear RFP.DepositorInsertH2B RFP
UseAAVTagsExpressionMutationPromoterhuman SynapsinAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV.U6gT28.4.EFS-NS.H2B-RFP
Plasmid#170365PurposePlasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Expressing nuclear RFP.DepositorInsertH2B-RFP
UseLentiviralTagsExpressionMutationPromoterEFS-NSAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV.U6gT28.3.EFS-NS.H2B-RFP
Plasmid#170364PurposePlasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Expressing nuclear RFP.DepositorInsertH2B-RFP
UseLentiviralTagsExpressionMutationPromoterEFS-NSAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-e1 sgRNA / hSpCas9
Plasmid#172830PurposeMammalian expression of a sgRNA targeting the exon 2 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the exon 2 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-e2 sgRNA / hSpCas9
Plasmid#172831PurposeMammalian expression of a sgRNA targeting the exon 2 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the exon 2 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_Camk2a sgRNA / hSpCas9
Plasmid#172839PurposeMammalian expression of a sgRNA targeting the intron 17 (last intron) of Camk2a (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 17 of Camk2a under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i5 sgRNA / hSpCas9
Plasmid#172829PurposeMammalian expression of a sgRNA targeting the intron 1 position 5 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i3 sgRNA / hSpCas9
Plasmid#172827PurposeMammalian expression of a sgRNA targeting the intron 1 position 3 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i2 sgRNA / hSpCas9
Plasmid#172826PurposeMammalian expression of a sgRNA targeting the intron 1 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xPP7-Qb model based
Plasmid#158203PurposeCassette of 10 different binding sites with high affinity to both PP7 and Qb phage coats protein, as computed by the ML model.DepositorInsert10 binding sites with high affinity to PP7 and QB coat proteins based on the machine-learning model
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJT46_GalL_cCDA1-BE3
Plasmid#145039PurposeExpresses cCDA1-BE3 in yeast cellsDepositorInsertcCDA1-BE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
Drosophila Nedd2-like caspase (DRONC)
Plasmid#157778PurposeE. coli expression vector pET23 with C-term 6xHis tagDepositorInsertDrosophila Nedd2-like caspase (DRONC) (Dronc Fly)
UseTags6x HISExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT139_GalL_nCDA1-VRERBE3
Plasmid#145102PurposeExpressing base editor nCDA1-VRERBE3 in yeast cellsDepositorInsertnCDA1-VRERBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A/D1135V/G1218R/R1335E/T1337R)PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT130_GalL_nCDA1-NGBE3
Plasmid#145094PurposeExpressing base editor nCDA1-NGBE3 in yeast cellsDepositorInsertnCDA1-NGBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT121_GalL_nCDA1-VQRBE3
Plasmid#145086PurposeExpressing base editor nCDA1-VQRBE3 in yeast cellsDepositorInsertnCDA1-VQRBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A, D1135V, R1335Q, T1337R)PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT92_GalL_nCDA1Δ188-BE3
Plasmid#145052PurposeExpresses nCDA1Δ188-BE3 in yeast cellsDepositorInsertnCDA1Δ188-BE3
UseCRISPRTagsExpressionYeastMutationpmCDA1(1-188AA); spCas9(D10A)PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT90_GalL_nCDA1Δ190-BE3
Plasmid#145050PurposeExpresses nCDA1Δ190-BE3 in yeast cellsDepositorInsertnCDA1Δ190-BE3
UseCRISPRTagsExpressionYeastMutationpmCDA1(1-190AA); spCas9(D10A)PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only