-
Plasmid#210132Purposeknock out BAK in mammalian cellsDepositorInsertBcl-2 homologous antagonist/killer (BAK1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgBAK-1
Plasmid#210131Purposeknock out BAK in mammalian cellsDepositorInsertBcl-2 homologous antagonist/killer (BAK1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas9_sgRNA_0
Plasmid#70763Purposeexpresses Ustilago maydis codon-optimized Cas9, contains U. maydis U6 promoter, is self-replicatingDepositorInsertsU6 promoter
cas9
tnos terminator
UseSelf-replicating in ustilago maydis, conferring c…TagsN-terminal NLS, C-terminal HA-tag +NLSExpressionMutationStreptococcus pyogenes gene codon-optimized for U…PromoterU6 from Ustilago maydis and synthetic otef promot…Available sinceNov. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_PPP6C_WT
Plasmid#81811PurposeGateway Donor vector containing PPP6C , part of the Target Accelerator Plasmid Collection.DepositorInsertPPP6C (PPP6C Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLCA.66/2272
Plasmid#22733DepositorInsertLox66-pU(delta)TK-EM7Neo-Lox2272
UseCre/Lox; Bac recombineeringTagsExpressionMutationThis vector was designed for assembling gene targ…PromoterAvailable sinceDec. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG3_M18
Plasmid#82356PurposeExpression plasmid coding for heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18.DepositorInsertmouse immunoglobulin heavy chain IgG3 isotype (Ighg3 Mouse)
UseTagsnoneExpressionMammalianMutationnonePromoterhEF1-HTLV promAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P2-MDM2 (17-125)
Plasmid#62063PurposeExpression of human MDM2 (17-125) in e. coliDepositorInsertMDM2 (MDM2 Human)
UseTagsGSTExpressionBacterialMutationcontains residues 17-125PromotertacAvailable sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
pBridge-tyrosinase.σ2
Plasmid#197415PurposeExpression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)DepositorInsertstyrosinase cytosolic tail
AP-2 σ2
UseTagsGAL4-DNA binding domain fragment, HA tag, and nuc…ExpressionYeastMutationencodes R42G substitution, contains silent substi…PromoterADH1 and MET25Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mScarlet-Giantin
Plasmid#196872PurposeExpresion of Giantin-fused to mScarlet. Used as Golgi markerDepositorInsertmScarlet-Giantin (GOLGB1 Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Halo-Tev- HSPB7 (zebrafish) WT
Plasmid#108916PurposeExpress WT Zebrafish Halo-Tev-HSPB7 in mammalian cellsDepositorInsertHalo-TEV-HSPB7 (hspb7 Zebrafish)
UseTagsHalo-TEVExpressionMammalianMutationPromoterSP6Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-N1-EXO1b-D173A
Plasmid#111622PurposeExpression of human catalitic dead Exonuclease 1 (EXO1) mCherry-tagged in mammalian cellsDepositorInsertExonuclease 1b D173A (EXO1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Mfn2-HA S27A
Plasmid#139193PurposeExpresses C-terminal HA tagged human Mfn2 S27A in mammalian cellsDepositorInsertMFN2 (MFN2 Human)
UseTags3HAExpressionMammalianMutationSerine 27 to AlaninePromoterCMV promoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Gfp-zf-vasa
Plasmid#167323PurposePlasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of zebrafish by in vitro transcription.DepositorInsertzf vasa 3'UTRs (ddx4 Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_TRAF5_WT
Plasmid#82167PurposeGateway Donor vector containing TRAF5 , part of the Target Accelerator Plasmid Collection.DepositorInsertTRAF5 (TRAF5 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mM_M18
Plasmid#91729PurposeExpression plasmid coding for heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18.DepositorInsertimmunoglobulin heavy constant mu (Ighm Mouse)
UseTagsExpressionMammalianMutationPromoterhEF1-HTLV promAvailable sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HF-BE3
Plasmid#87439PurposeExpresses HF-BE3 with C-terminal NLS in mammalian cellsDepositorInsertHF-BE3 (Apobec1 Rat, Bacillus phage PBS2, Human)
UseTagsExpressionMammalianMutationMammalian codon-optimized Cas9-HFPromoterCMVAvailable sinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGM234
Plasmid#220177PurposeExpresses exogenous wild-type NAC complex members (NACA and BTF3)DepositorUseLentiviralTags3xFLAG (NACA); 6xHis (BTF3)ExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
PLL5.0-Anillin ShRNA-GFP-AnillinFL
Plasmid#187272PurposeLentiviral expression of shRNA targeting Anillin + reconstitution of Anillin full lengthDepositorInsertAnillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
UseLentiviralTagsEGFPExpressionMutationPromoterU6, CMVAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Mfn2-HA S27D
Plasmid#139194PurposeExpresses C-terminal HA tagged human Mfn2 S27D in mammalian cellsDepositorInsertMFN2 (MFN2 Human)
UseTags2HAExpressionMammalianMutationSerine 27 to Aspartic AcidPromoterCMV promoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only