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Anti-Neuroligin-1 [N97A/31R]

Plasmid

#114554

Purpose

Mammalian Expression Plasmid of anti-Neuroligin-1 (Rat). Derived from hybridoma N97A/31.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Neuroligin-1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Nlgn1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pESC-Leu-SopB(C460S)

Plasmid

#183671

Purpose

Inducible Salmonella Typhimurium SopB (catalytically inactive) for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Use

Tags

Expression

Yeast

Mutation

C460S

Promoter

GAL1

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-SPdCas9-DNMT3A(E752A)-2A-Blast

Plasmid

#71218

Purpose

dCas9 fused to the human DNMT3A catalytically inactive domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-DNMT3A(E752A)

Use

Lentiviral

Tags

SPdCas9-DNMT3A(E752A)

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO_AU1.C.HA_mCherry-NLS

Plasmid

#178209

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.C.HA and Nuclear Localization Signal

Expression

Mutation

Promoter

eF1a

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pPW3762 : CMVd3-HsIRE1(K121Y)-HaloTag

Plasmid

#185684

Purpose

Low-level transient expression of IF1 mutant HsIRE1 with a C-terminal HaloTag.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERN1 (ERN1 Synthetic, Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

K121Y

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 MCS-HIPK3 Exon

Plasmid

#69905

Purpose

Expresses the human HIPK3 exon 2 circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

HIPK3 (HIPK3 Fly, Human)

Use

Tags

Expression

Mammalian

Mutation

Expresses aa1-194 of HIPK3

Promoter

CMV

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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Flrt1-Fc-His

Plasmid

#72073

Purpose

Expresses the extracellular region of the FLRT1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Flrt1 (Flrt1 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pPSV39-dddAI

Plasmid

#186557

Purpose

Broad host range regulated expression of the DddA immunity determinant (DddAI)

Depositor

Joseph Mougous

Article

Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

Insert

dddAI

Use

Tags

Expression

Bacterial

Mutation

Promoter

lacUV5

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-hGATE-16

Plasmid

#87870

Purpose

Express a EGFP-human GATE-16 in mammalian cells

Depositor

Eiki Kominami, Isei Tanida

Article

Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

Insert

GATE-16 (GABARAPL2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pET32a-HD39Q

Plasmid

#11514

Depositor

Pamela Bjorkman

Article

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

huntingtin, exon 1, 39 glutamines (HTT Human)

Use

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

huntingtin exon 1 with 39 glutamines, vector m…

Promoter

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
Myc-Rab35 S22N

Plasmid

#47435

Depositor

Peter McPherson

Article

Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

Insert

Rab35 S22N (RAB35 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

S22N inactive

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO_NWS.Ollas.S_mCherry-NLS

Plasmid

#178279

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

NWS.Ollas.S and Nuclear Localization Signal

Expression

Mutation

Promoter

eF1a

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_HSV.S.V5_mCherry-NLS

Plasmid

#178278

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

HSV.S.V5 and Nuclear Localization Signal

Expression

Mutation

Promoter

eF1a

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_HSV.Ollas.V5_mCherry-NLS

Plasmid

#178277

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

HSV.Ollas.V5 and Nuclear Localization Signal

Expression

Mutation

Promoter

eF1a

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_HSV.NWS.S_mCherry-NLS

Plasmid

#178273

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

HSV.NWS.S and Nuclear Localization Signal

Expression

Mutation

Promoter

eF1a

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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Tet-myr-CSK K222R-GFP

Plasmid

#83471

Purpose

Lentiviral vector for doxycycline-inducible expression of membrane targeted kinase dead CSK in mammalian cells

Depositor

Barry Gumbiner

Article

Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

Insert

CSK (CSK Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

K222R (kinase dead)

Promoter

doxycycline-inducible promoter

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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anti-GFP scFv [N86/38] with sortase tag

Plasmid

#204419

Purpose

Mammalian Expression of anti-GFP scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N86/38.

Depositor

Jeff Lichtman

Article

Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

Insert

Recombinant mouse scFv targeting GFP (Aequorea victoria)

Use

Tags

6xHis tag and Sortase tag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-Mkk7-JNK2 fusion-pcw107

Plasmid

#64618

Purpose

Mkk7-JNK2 fusion - barcoded activating construct of JNK pathway

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

Map2k7 linker MAPK9

Use

Lentiviral

Tags

FLAG

Expression

Mutation

mouse-human fusion

Promoter

PGK

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
CiDr-VSP L223F/C302S-mCherry pcDNA3.1 (eVSP CSmutant)

Plasmid

#140893

Purpose

Expresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cel

Depositor

Yasushi Okamura

Article

Kawanabe et al J Gen Physiol. 2020 May 4;152(5). pii: 1

Insert

CiDr-VSP L223F/C302S-mCherry (vsp, tpte Ciona intestinalis, Zebrafish)

Use

Tags

mCherry

Expression

Mammalian

Mutation

changed 223L to F in Dr-VSP (zebrafish TPTE), cha…

Promoter

Available since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNotch1#1/Cre

Plasmid

#193223

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch1 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgNotch1#1 (Notch1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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