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pSB3K3 containing mRFP1
Plasmid#118083PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertpromoter, RBS, mRFP1
UseEscherichia coliTagsExpressionMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-SspB-HaloTag-TIAM1-DHPH
Plasmid#176127PurposeHeterodimerization with iLID, Rac1 activationDepositorInsertTIAM1 (TIAM1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA FLAG TORC3
Plasmid#22976DepositorInsertTORC3 (CRTC3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Beclin1 CC
Plasmid#24403DepositorInsertBeclin1 (BECN1 Human)
UseTagsHisExpressionBacterialMutationContains the CC domain (aa 151-242)PromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-dSaCas9-2xNLS
Plasmid#107319PurposeExpresses SpCas9 fused to dSaCas9 in mammalian cellsDepositorInsertSpCas9-SaCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 is fused to nuclease dead SaCas9 (D10A, N5…PromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-AC3CtermGenomic-mCherry
Plasmid#105250Purposemouse mCherry (with STOP and extra-sequence) with homology arms for Adcy3DepositorInsertmCherry (with STOP and extra-sequence) with homology arms for mouse Adcy3 (Adcy3 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSW609 - N001
Plasmid#115993Purposeintermediate vector for GreenGate cloning method, contains modules from A-FDepositorInsertH-A adapter:pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseUnspecifiedTagsHDEL and SP(ER)ExpressionMutationPromoterAvailable sinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-CRY2(D387A)-mCherry
Plasmid#58369PurposeLight insensitive CRY2 mutantDepositorInsertCRY2[1-498] (D387A) (CRY2 Mustard Weed)
UseTagsmCherryExpressionMammalianMutationD387APromoterCMVAvailable sinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBT311_pH11-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5
Plasmid#52544PurposeTargeting vector for homologous recombination in mouse ES cells; Hipp11 locusDepositorInsertsPhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQE81L-KOFP7
Plasmid#160330PurposeThis plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is comparable to EGFP.DepositorInsertKOFP7
UseTags6xHis tagExpressionBacterialMutationPromoterT5Available sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
asPink chromoprotein promoter-less
Plasmid#118005PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertRBS, asPink
UseEscherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
iSH-iRFP670-sspB
Plasmid#176102PurposeHeterodimerization with iLID, phosphatidylinositol 3-kinase derived iSHDepositorInsertphosphatidylinositol 3-kinase (Pik3r1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing anti SARS-CoV-2_RBD sybody Sb#45
Plasmid#153526PurposeExpression of the anti SARS-CoV-2_RBD sybody Sb#45DepositorInsertSb#45
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
VPR_dCas9
Plasmid#134601PurposeExpress CHO codon-optimized dCas9 fused to VPR for CRISPR activation. 2A linked ZsGreen1 markerDepositorInsertVPR-dCas9-2A-ZsGreen-DR
UseTagsVPR and ZsGreen1-DRExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
1119 pBabe puroL HA FKHR AAA
Plasmid#9025DepositorInsertFKHR AAA (FOXO1 Human)
UseRetroviralTagsHAExpressionMammalianMutationT24A S256A S319APromoterAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDest-521
Plasmid#159688PurposeGateway destination vector for E. coli protein expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLR5-CAG-44B12mut23(Ser5ph)-SNAP
Plasmid#174877PurposePiggyBac transposon vector for expression of RNAPII Ser5ph mintbodyDepositorInsertRNAPII Ser5ph mintbody-SNAP
UsePiggybac vectorTagsExpressionMammalianMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHS1398
Plasmid#205971PurposeMWEE01000013 Cas5-HNH crRNA expression in pCOLADuet-1DepositorInsertCas5-HNH crRNA
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
