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Plasmid#132618Purposeexpresses human TSC1 (939-992) in E. coliDepositorInsertTSC1 (939-992)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
K8.1Pr-pGL4.16-OriLyt
Plasmid#131038PurposeFirefly Luciferase reporter for K8.1 promoter from KSHV with the lytic origin of replicationDepositorInsertKSHV Lytic Origin of replication
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0015
Plasmid#117551PurposeLevel 1 Golden Gate Cassette: SaCas9 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + SaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S+5'UTR OMEGA (pICH51288)Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-vdh
Plasmid#128134PurposeThe cell-free adapted backbone, pBEAST, expressing vdh (the enzyme converting benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertvdh
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-bphD
Plasmid#128138PurposeThe cell-free adapted backbone, pBEAST, expressing bphD (the enzyme converting 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertbphD
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15b-GBdm
Plasmid#129690Purposeexpress E. coli GreB E82C/C68SDepositorInsertGreB E82C C68S (greB E. coli)
UseTagshis6ExpressionBacterialMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD286
Plasmid#125074PurposeExpression of SO_4622 under PtacDepositorUseSynthetic BiologyTagsExpressionBacterialMutationS286L- Please see depositor comments belowPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD276
Plasmid#125069PurposeExpression of SO_0621 under PtacDepositorUseSynthetic BiologyTagsExpressionBacterialMutationS286L- Please see depositor comments belowPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD250.2
Plasmid#125077PurposeExpression of torR under PLtetO-1, output promoter PtorCAD129DepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR300.3
Plasmid#125079PurposeExpression of uhpA under PLtetO-1, output Promoter PuhpT99DepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBDU-Atg17(F317D, I325A)
Plasmid#122275PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationPhenylalanine 317 to Aspartic acid; Isoleucine 32…PromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0078
Plasmid#117645PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113), sgRNA{AT1G73460}(pEPOR1CB0099) and sgRNA{AT1G73460}(pEPOR1CB0110) to Protein kinase superfamily protein.DepositorInsert[35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(F317H)
Plasmid#122269PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationPhenylalanine 317 to HistidinePromoterAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBDU-Atg17(I325A)
Plasmid#122274PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationIsoleucine 325 to alaninePromoterAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-PIWIL2 ts2
Plasmid#115870PurposePIWIL2 knockdownDepositorInsertPIWIL2 shRNA (PIWIL2 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(F317D, I325A)
Plasmid#122273PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationPhenylalanine 317 to Aspartic acid; Isoleucine 32…PromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(I325A)
Plasmid#122272PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationIsoleucine 325 to alaninePromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(F317Q)
Plasmid#122271PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationPhenylalanine 317 to glutaminePromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(F317D)
Plasmid#121391PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationPhenylalanine 317 to Aspartic acidPromoterADH1 promoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(F317G)
Plasmid#122268PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationPhenylalanine 317 to glycinePromoterAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only