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Plasmid#209974PurposeContains Level 0 Part: Terminator (TpepN) for the construction of Level 1 plasmidsDepositorInsertTerminator (T_pepN)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPC53
Plasmid#209962PurposeContains Level 0 Part: 5' Connector (5C1CSN with double terminator Tcat+sai) for the construction of Level 1 plasmidsDepositorInsert5' Connector (5C1CSN_Tcat+sai)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
EphA1-SAM-Cry2olig
Plasmid#176600PurposeEphrinA1 RTK fusion to Cry2olig - includes SAM domainDepositorInsertEphrinA1-Cry2olig-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
EphA1-RTK-Cry2olig
Plasmid#176601PurposeEphrinA1 RTK fusion to Cry2olig - RTK domain onlyDepositorInsertEphA1rtk-Cry2olig-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pm-EphA1-SAM-Cry2olig
Plasmid#176684Purposemembrane localized EphrinA1 RTK fusion to Cry2olig - includes SAM domainDepositorInsertPM-EphA1-Cry2olig-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pALS3-Ma-sfGFP-WT
Plasmid#212119PurposeFluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.DepositorInsertsSUMO-sfGFP-WT
M. alvus Pyl-tRNA(6)
UseTagsHis6ExpressionBacterialMutationPromoteraraC and lppAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC2483 (pSpy1C)
Plasmid#220248Purposereplicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
OgeuIscB ωRNA-v2
Plasmid#220958Purposehuman U6-driven expression of ωRNA-v2 scaffold with BsmBI for guide cloingDepositorInsertOgeuIscB-ωRNA-v2 scaffold with BsmBI for guide cloning
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
His_CL7_2NLS_iCas12a-4NLS
Plasmid#222971PurposeBacterial expression of NLS-rich iCas12a constructDepositorInsertHis_CL7_2NLS_iCas12a-4NLS
UseCRISPRTagsCL7 and HisExpressionBacterialMutationPromotertacAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141B-ge4.1
Plasmid#205144PurposeGuide RNA expression cassette harboring AtU3 promoter, ge4.1 5’ modification, and ge4.1 3’ modificationDepositorInsertgRNA cloning site
UseCRISPRTagsExpressionMutationPromoterAtU3Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCM4.4
Plasmid#217648Purposean ermE*p-cas9-driven expression, as an alternative plasmidDepositorInsertCodon Optimized SpCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
dSp-p65 Full (1-261)
Plasmid#99654PurposeExpresses dSp Cas9 fused to p65 activation domainDepositorArticleInsertdCas9
UseTagsp65 activation domainExpressionMammalianMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-BRIPO
Plasmid#215542PurposeBRIPO (Bioluminescent Red Indicator for Potassium)DepositorInsertBRIPO
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0.423
Plasmid#203947PurposeLevel 0 part. 2,4-diacetylphloroglucinol (DAPG)-inducible phlF_PphlF promoter.DepositorInsertphlF-PphlF
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-C-PrLD
Plasmid#216398PurposeExpresses 6xHis-tagged C-terminal prion-like domains of Efg1DepositorInsertEfg1-C-PrLD
UseTags6xHis-tagsExpressionBacterialMutationPromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-DBD
Plasmid#216399PurposeExpresses 6xHis-tagged DBD of Efg1DepositorInsertEfg1-DBD
UseTags6xHis-tagsExpressionBacterialMutationdeleted amino acids 2-181 and 357-554PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBT120a17-sg-del
Plasmid#214347PurposeExpresses pIIIDepositorInsertgIII-xLuxAB
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT87
Plasmid#204489PurposeCAG promoted WBeta recombinaseDepositorInsertWBeta recombinase
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBTK1061
Plasmid#209550PurposeBTK assembled plasmid for integrating a KanR+E2-Crimson cassette into the S. alvi genome in place of the clpA geneDepositorInsertE2-Crimson cassette flanked with homology arms for recombination into the S. alvi wkB2 genome to replace clpA (SALWKB2_RS04465)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKM493
Plasmid#140191PurposeORBIT integrating plasmid for C-terminal tagging with cleavable EGFP.DepositorInsertTEV-Flag-Gly4-eGFP
UseTagsFlag + eGFPExpressionBacterialMutationPromoterPGroELAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only