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Human_TFAM_HMGA_pET28

Plasmid

#167326

Purpose

Expresses TFAM HMGA mutant in bacterial cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Tags

6XHis

Expression

Bacterial

Mutation

Truncation mutant containing HMGA domain (43-122)

Promoter

T7

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_HMGA_linker_pET28

Plasmid

#167327

Purpose

Expresses TFAM HMGA+linker mutant in bacterial cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Tags

6XHis

Expression

Bacterial

Mutation

Truncation mutant containing HMGA and linker doma…

Promoter

T7

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_HMGB_ctail_pET28

Plasmid

#167330

Purpose

Expresses TFAM HMGB+C-tatil mutant in bacterial cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Tags

6XHis

Expression

Bacterial

Mutation

Truncation mutant containing HMGB and C-tail doma…

Promoter

T7

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_dC_pET28

Plasmid

#167328

Purpose

Expresses TFAM dC-tail mutant in bacterial cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Tags

6XHis

Expression

Bacterial

Mutation

Truncation mutant containing HMGA, linker and HMG…

Promoter

T7

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pRS406 ATG1-ATG7-3'UTR

Plasmid

#166847

Purpose

Expresses Atg1 with ATG7 3'UTR in yeasts.

Depositor

Daniel Klionsky

Article

Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

Insert

ATG1 (ATG1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

ATG1 Promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-kcnt2a

Plasmid

#164963

Purpose

Zebrafish kcnt2a gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnt2a (kcnt2 Zebrafish)

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-Coh-ddFLN4-ELP(MV7E2)3-His-ybbR

Plasmid

#157672

Purpose

Expression of Coh (type III from Ruminococcus flavefaciens) with ddFLN4, ELP linker, His6x tag, and ybbR Tag.

Depositor

Michael Nash

Article

Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

Insert

Cohesin (R.f) with ddFLN4, ELP, Histag and ybbR tag

Use

Tags

ddFLN4, ELP, Histag and ybbR tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFA-DAB1

Plasmid

#162691

Purpose

Expresses DabAB1 inorganic carbon transport complex

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

DabAB1 inorganic carbon transport complex

Use

Tags

Expression

Bacterial

Mutation

Promoter

PLtet0-1 promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pSWD95Y14D

Plasmid

#159098

Purpose

CckA FRET Sensor with cyclic-di-GMP point mutant

Depositor

Seth Childers

Article

Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

Insert

CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691(Y514D), Linker SNVTRHRSAT, mRuby3

Use

Tags

Expression

Bacterial

Mutation

Mutated Y514 to D514

Promoter

Xylose

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSWD95D293A

Plasmid

#159099

Purpose

CckA FRET Sensor with DITE motif mutant

Depositor

Seth Childers

Article

Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

Insert

CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691 (D293A), Linker SNVTRHRSAT, mRuby3

Use

Tags

Expression

Bacterial

Mutation

CckA D293 mutated to A293 and V199I on mRuby3 (P…

Promoter

Xylose

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pET_4E2H.210

Plasmid

#155212

Purpose

Expresses 4E2H.210 de novo protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4E2H.210

Use

Tags

6x His Tag

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-TYK2VF/PA-VSV

Plasmid

#139351

Purpose

Expression of the double mutant of TYK2-V678F-P1104A

Depositor

Sandra Pellegrini

Article

Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

Insert

TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)

Use

Tags

VSV

Expression

Mammalian

Mutation

V362F-V678F-P1104A

Promoter

CMV

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-Centrin3

Plasmid

#136855

Purpose

Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Centrin3

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetDBl-CLIP-Centrin3

Plasmid

#136856

Purpose

Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Centrin3

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetDBl-CLIP-FOP

Plasmid

#136846

Purpose

Mammalian expression of the centrosomal protein FGFR N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-FGFR

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetDBl-CLIP-POC5

Plasmid

#136836

Purpose

Mammalian expression of the centrosomal protein POC5 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-POC5 (POC5 Synthetic, Human)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-FOP

Plasmid

#136845

Purpose

Mammalian expression of the centrosomal protein FGFR N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-FGFR

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-STIL

Plasmid

#136844

Purpose

Mammalian expression of the centrosomal protein STIL N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-STIL (STIL Synthetic, Human)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-POC5

Plasmid

#136835

Purpose

Mammalian expression of the centrosomal protein POC5 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-POC5 (POC5 Synthetic, Human)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-POC5

Plasmid

#136787

Purpose

Mammalian expression of the centrosomal protein POC5 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-POC5 (POC5 Synthetic, Human)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge