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Plasmid#166701Purposeexpresses NEDD4L WW1 in bacterial cellsDepositorInsertNEDD4L WW1 domain
UseTagsGST, HRV 3C site, and Thrombin siteExpressionBacterialMutationPromotertac promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PP-NEDD4L-WW2(385-418)
Plasmid#166702Purposeexpresses NEDD4L WW2 in bacterial cellsDepositorInsertNEDD4L WW2 domain
UseTagsGST, HRV 3C site, and Thrombin siteExpressionBacterialMutationPromotertac promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PP-NEDD4L-WW3(497-530)
Plasmid#166703Purposeexpresses NEDD4L WW3 in bacterial cellsDepositorInsertNEDD4L WW3 domain
UseTagsGST, HRV 3C site, and Thrombin siteExpressionBacterialMutationPromotertac promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PP-NEDD4L-WW4(548-581)
Plasmid#166704Purposeexpresses NEDD4L WW4 in bacterial cellsDepositorInsertNEDD4L WW4 domain
UseTagsGST, HRV 3C site, and Thrombin siteExpressionBacterialMutationPromotertac promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCA528-PPxY1-GS-WW3e
Plasmid#166705Purposeexpresses an N-terminal fusion of AMOT PPxY1 to NEDD4L WW3 in bacterial cellsDepositorInsertAMOT PPxY1 fused to NEDD4L WW3
UseTagsHis-SUMOExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-Flag-NEDD4L∆WW1
Plasmid#166707Purposeexpresses NEDD4L with inactivating mutation in first WW domain in mammalian cellsDepositorInsertNEDD4L W221A
UseTagsFLAGExpressionMammalianMutationchanged tryptophan 221 to alaninePromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (E143amb)-His
Plasmid#168046PurposeExpresses anticalin targeting CTLA-4 with the codon encoding E143 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationGlu 143 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (S87amb)-His
Plasmid#168044PurposeExpresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationSer 87 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (N116amb)-His
Plasmid#168045PurposeExpresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationAsn 116 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (N21amb)-His
Plasmid#168041PurposeExpresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationAsn 21 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Fgβ-anticalin-His-ybbr
Plasmid#168040PurposeExpresses anticalin targeting CTLA-4 with Fgβ peptide at the N terminus and 6x-His tag and ybbr tag at the C terminusDepositorInsertAnticalin
UseTags6x Histag, Fgβ peptide, and ybbr tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-HisTim12_CtoA
Plasmid#170281PurposeExpresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli productionDepositorInsertyeast Tim12 (TIM12 Budding Yeast)
UseTagsTEV-cleavable N-terminal His tag (on backbone)ExpressionBacterialMutationmutated two non-conserved Cys to AlaPromoterAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (I55amb)-His
Plasmid#168042PurposeExpresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationIle 55 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 ΔH1 Δ5H
Plasmid#163582Purposeexpress Dcp2 ΔH1 Δ5H at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationL255A; mutate 440-450, 489-500, 701-708, 827-831,…PromoterDCP2 promoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2C Δ5H
Plasmid#163581Purposeexpress Dcp2C Δ5H at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationTruncation of aa1-300; mutate aa440-450, aa489-50…PromoterDCP2 promoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH_CMV_dimer_mKate2
Plasmid#167333PurposeExpresses mKate2 fused to TFAM no-dimerization mutant in mammalian cellsDepositorInsertTFAM (TFAM Human)
UseLentiviralTagsmKate2ExpressionMammalianMutationChanged K95A, Y99F, E106A, E112A and R116APromoterCMVAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH_CMV_HMGA_mKate2
Plasmid#167334PurposeExpresses mKate2 fused to TFAM HMGA mutant in mammalian cellsDepositorInsertTFAM (TFAM Human)
UseLentiviralTagsmKate2ExpressionMammalianMutationTruncation mutant containing MTS (1-42) and HMGA …PromoterCMVAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH_CMV_L6_mKate2
Plasmid#167332PurposeExpresses mKate2 fused to TFAM L6 mutant in mammalian cellsDepositorInsertTFAM (TFAM Human)
UseLentiviralTagsmKate2ExpressionMammalianMutationChanged K136, H137, K139, R140, K146 and K147 to …PromoterCMVAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA-DAB2
Plasmid#162690PurposeExpresses DabAB2 inorganic carbon transport complexDepositorInsertDabAB2 inorganic carbon transport complex
UseTagsExpressionBacterialMutationPromoterPLtet0-1 promoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human_TFAM_dHMGA_pET28
Plasmid#167329PurposeExpresses TFAM dHMGA mutant in bacterial cellsDepositorInsertTFAM (TFAM Human)
UseTags6XHisExpressionBacterialMutationTruncation mutant containing linker, HMGB, and C-…PromoterT7Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only