Addgene

Showing: 7981 - 8000 of 24299 results

Results per page:

Sort results by:

pMC3-yeGFP

Plasmid

#176181

Purpose

yeast MoClo level-0 part plasmid type 3 containing yeast-enhanced GFP

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

yEGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR R4 ATCC LacZ GCTT

Plasmid

#173668

Purpose

Destination vector for Golden Gate Cloning assemblies, blue-white screening

Depositor

Eric Marois

Article

Green et al Elife. 2023 Dec 5;12:e93142. doi: 10.755

Type

Empty backbone

Use

Tags

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGBW-m4092686

Plasmid

#153895

Purpose

Bacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Bacterial

Mutation

Nearly native encoding;inactivating mutations in …

Promoter

Promoter | t7 consensus from NEB, G is +1

Available since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pQTEV-SCP2

Plasmid

#31336

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

sterol carrier protein 2 (SCP2 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
aq-CFP

Plasmid

#36073

Depositor

Narasimhan Gautam

Article

Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

Insert

G protein subunit alpha q (Gnaq Mouse)

Use

Tags

CFP

Expression

Mammalian

Mutation

CFP inserted between amino acid 124 and 125

Promoter

CMV

Available since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only
pLenti-CaMKIIa-C1C2-EYFP

Plasmid

#35519

Depositor

Karl Deisseroth

Article

Kato et al Nature. 2012 Jan 22. doi: 10.1038/nature

Insert

ChR1-ChR2 Chimera

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

June 19, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pT2-cryR;clecl14aCE1-P1Egfp

Plasmid

#90157

Purpose

contains clec14a enhancer sequence, and clec14a specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

clec14aE1P1Egfp

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-FRB-HA no-pA

Plasmid

#82594

Purpose

3' entry vector containing HA epitope fused to C-terminus of TOR FKBP and rapamycin binding (FRB) domain without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FRB-HA (no pA)

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGBW-m4046841

Plasmid

#145578

Purpose

Bacterial Expression plasmid for SARS-CoV-2 helicase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;FLAG_2

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4046854

Plasmid

#145759

Purpose

Bacterial Expression plasmid for SARS-CoV-2 leader protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 leader protein (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
BAT-lux

Plasmid

#20890

Depositor

Stefano Piccolo

Article

Maretto et al Proc Natl Acad Sci U S A. 2003 Mar 18. 1

Insert

7xTCF binding sites, Siamois minimal promoter

Use

Luciferase

Tags

Expression

Mammalian

Mutation

7 multimerized TCF-binding elements fused upstrea…

Promoter

Available since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
pGBW-m4046832

Plasmid

#145691

Purpose

Bacterial Expression plasmid for SARS-CoV-2 nsp7

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;StrepII

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
mEmerald-MCM5-N-22

Plasmid

#54170

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM5

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
Psy074

Plasmid

#99504

Purpose

Bacterial expression of LIPS M1+TE

Depositor

Jay Keasling

Article

Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

Insert

LIPS M1+TE

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
XE152 GFP-XDpr-CS2+

Plasmid

#16770

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

GFP-XDpr (dact1-b Frog)

Use

Xenopus expression

Tags

gfp

Expression

Mutation

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA-NES-ZapCmR1.1

Plasmid

#59014

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

Insert

NES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor

Use

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pEBB-BT-Ubiquitin

Plasmid

#36098

Depositor

Ezra Burstein

Article

Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub

Insert

Ubiquitin (RPS27A Human)

Use

Tags

TB-tag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 15, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
p7128 pHAGE-P-CMVt-N-HA-GAW-HLA-A

Plasmid

#100154

Purpose

Expresses HLA-A

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

HLA-A (HLA-A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p6607 MSCV-IP N-HAonly PTPN18

Plasmid

#35007

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

PTPN18 (PTPN18 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

Feb. 9, 2012

Availability

Academic Institutions and Nonprofits only
pGBW-m4046953

Plasmid

#145764

Purpose

Bacterial Expression plasmid for SARS-CoV-2 ORF7b

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF7b (ORF7b Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge