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Plasmid#71360Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:C176ADepositorInsertHP0518 (HP0518 changed Cysteine 176 to Alanine)
UseTagsHistidine tagExpressionBacterialMutationPromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330_H160A/pET28b(+) N-tag
Plasmid#71359Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:H160ADepositorInsertHP0518 (HP0518 changed Histidine 160 to Alanine)
UseTagsHistidine tagExpressionBacterialMutationPromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330_W158A/pET28b(+) N-tag
Plasmid#71358Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:W158ADepositorInsertHP0518 (HP0518 changed Tryptophan 158 to Alanine)
UseTagsHistidine tagExpressionBacterialMutationPromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330_H155A/pET28b(+) N-tag
Plasmid#71357Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:H155ADepositorInsertHP0518 (HP0518 changed Histidine 155 to Alanine)
UseTagsHistidine tagExpressionBacterialMutationPromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330_Y132A/pET28b(+) N-tag
Plasmid#71355Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:Y132ADepositorInsertHP0518 (HP0518 changed Tyrosine 132 to Alanine)
UseTagsHistidine tagExpressionBacterialMutationPromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5x3-hMeCP2-delN256-G273X
Plasmid#48090PurposeBacterial expression of GST tagged AT-Hook2-G273XDepositorInsertMethyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
UseTagsGSTExpressionBacterialMutationdeleted the N-terminal 256 codons, expresses AT-H…PromoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5x3-hMeCP2-delN256-R270X
Plasmid#48089PurposeBacterial expression of GST tagged AT-Hook2-R270XDepositorInsertMethyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
UseTagsGSTExpressionBacterialMutationdeleted the N-terminal 256 codons, expresses AT-H…PromoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMX-Gal4-hMeCP2-G273X-R111G
Plasmid#48087PurposeMammalian expression of Gal4 tagged MeCP2-G273X-R111GDepositorInsertMethyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
UseTagsGal4ExpressionMammalianMutationR111G mutant MeCP2, expresses AT-hook G273XPromoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag4A-MMP8 (E87D)
Plasmid#29547DepositorInsertMetalloproteinase protein 8 (MMP8 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationE87DPromoterAvailable sinceSept. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
PCMV-Tag4A-MMP8 (E138Q)
Plasmid#29549DepositorInsertMetalloproteinase protein 8 (MMP8 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationE138QPromoterAvailable sinceJuly 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
Pet28a His6-HSPB7 (zebrafish) WT
Plasmid#108917PurposeExpress WT Zebrafish His6-HSPB7 in bacteriaDepositorInsertHis6-HSPB7 (hspb7 Zebrafish)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone
Plasmid#216152PurposeExpresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11']DepositorInsertmScarlet with cryptic exon and C-terminal FLAG
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GenEPi
Plasmid#140236PurposeMammalian expression of Piezo1-based fluorescent force sensor GenEPiDepositorInsertGenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)
UseTagsGCaMP 6s RS1 EF4ExpressionMammalianMutationPromotersimian CMV IE94Available sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas-MmdCas12m-CBE1
Plasmid#192284PurposeMmdCas12m-CBE1 expressed under a constitutive promoterDepositorInsertMmdCas12m-CDA-UGI
UseCRISPRTagsExpressionBacterialMutationD485APromoterPJ23108Available sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
InsRA-eGFP
Plasmid#79795PurposeInsulin receptor isoform A with a inter-domain eGFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.DepositorInsertInsRA (INSR Human)
UseTagseGFP extracellularExpressionMammalianMutationPromoterCMVAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-EBF1
Plasmid#96965PurposeDox-inducible expression of Ebf1 in mammalian cellsDepositorInsertEbf1 (Ebf1 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMIG-FLAG-MLL-AF9
Plasmid#71443PurposeRetroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-GFPDepositorInsertMLL-Af9 (KMT2A Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterMSCVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGmCherry-gRNA
Plasmid#87110PurposegRNA cloning vector with CAGmCherryDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMmaPylT_EF1_CRFR1-A95TAG-HA
Plasmid#174900Purposeamber suppression mediated CRFR1 A95TAG expression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertCRFR1 (Crhr1 Rat)
UseTagsHAExpressionMammalianMutationA95TAGPromoterEF1Available sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL21-VEX
Plasmid#158230PurposeRetroviral expression plasmid of sgRNA with violet-excited fluorescent protein (VEX)DepositorTypeEmpty backboneUseCRISPR and RetroviralTagsExpressionMammalianMutationPromoterU6 promoter for crRNA expression and EFS promoter…Available sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only