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Plasmid#174494Purposebacterial expression of Drosophila Sep1 fused to monomeric superfolder GFPDepositorInsertSep1 (Sep1 Fly)
UseTagsHis6-TEV and msfGFPExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mcherry-WIPI2dH85E-cs-TEV-STREP
Plasmid#171838PurposeMammalian expression of human WIPI2d H85E with N-terminal mCherry and C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrep and mCherryExpressionMammalianMutationH85EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mcherry-WIPI2dK88E-cs-TEV-STREP
Plasmid#171839PurposeMammalian expression of human WIPI2d K88E with N-terminal mCherry and C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrep and mCherryExpressionMammalianMutationK88EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mcherry-WIPI2dI92E-cs-TEV-STREP
Plasmid#171840PurposeMammalian expression of human WIPI2d I92E with N-terminal mCherry and C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrep and mCherryExpressionMammalianMutationI92EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mcherry-WIPI2dC93E-cs-TEV-STREP
Plasmid#171841PurposeMammalian expression of human WIPI2d C93E with N-terminal mCherry and C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrep and mCherryExpressionMammalianMutationC93EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mcherry-WIPI2dR108E-cs-TEV-STREP
Plasmid#171842PurposeMammalian expression of human WIPI2d with N-terminal mCherry and C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrep and mCherryExpressionMammalianMutationR108EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mcherry-WIPI2dR125E-cs-TEV-STREP
Plasmid#171843PurposeMammalian expression of human WIPI2d R108E with N-terminal mCherry and C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrep and mCherryExpressionMammalianMutationR125EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mcherry-WIPI2dK128E-cs-TEV-STREP
Plasmid#171844PurposeMammalian expression of human WIPI2d K128E with N-terminal mCherry and C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrep and mCherryExpressionMammalianMutationK128EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis(TEV)-Drp1(K38A)-StrepII
Plasmid#174423PurposeExpresses human Drp1 Isoform 3 with K38A mutation in bacteriaDepositorInsertDrp1
UseTags6xHis(TEV) and StrepIIExpressionBacterialMutationK38APromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
PMP34-hLOV-TEVcs-GAL4bd-VP64-V5
Plasmid#170997PurposeHiLITR transcription factor with full-length PMP34 targeting sequence (Peroxisome)DepositorInsertPMP34(FL)-NNES-hLOV-TEVcs(ENLYFQ-M)-GAL4bd-VP64-V5 (SLC25A17 Synthetic)
UseLentiviral and Synthetic BiologyTagsV5ExpressionMammalianMutationPromoterEf1-alphaAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-SNAP-tag_dead_mt
Plasmid#167270PurposeOverexpression of the dead variant (C145A) of SNAP-tag in E. coliDepositorInsertSNAP-tag
UseTagsHis x10ExpressionBacterialMutationC145APromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-SNAP-tag_fast_dead_mt
Plasmid#167272PurposeOverexpression of the fast (E30R) dead variant (C145A) of SNAP-tag in E. coliDepositorInsertSNAPf-tag
UseTagsHis x10ExpressionBacterialMutationE30R-C145APromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFD-MBP-CS(TEV)-StrepTag-II-AI(TVMV)
Plasmid#137110PurposeiFLinkC Linker cloningDepositorInsertMBP-CS(TEV)-StrepTag-II-AI(TVMV)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
MBP-TEV-Gly-RadA∆C46
Plasmid#128702PurposeExpresses PhoRadA intein split at residue 126 from the N-terminalDepositorInsertRadA
UseTagsHisx6-MBPExpressionBacterialMutationPromoterT7Available sinceAug. 12, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKK-RNAi-nucCHERRYmiR-FLAG-TEV
Plasmid#105809PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal FLAG.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsFLAG-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
Halo-Tev-Akt3 C119S-2X Flag
Plasmid#87608Purposeexpresses halo tagged at the N-terminus and 2 tandem flag tags at the C-terminus of C119S mutant of human Akt3 proteinDepositorInsertAKT3 isoform 2 (AKT3 Human)
UseZebrafish (danio rerio) expressionTags2 tandem flag tags and His-Halo-TevExpressionMammalianMutationcysteine 119 residue mutated to serinePromoterAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Ala-eK-Flv
Plasmid#83393Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Ile-eK-Flv
Plasmid#83392Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Tyr-eK-Flv
Plasmid#83391Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Trp-eK-Flv
Plasmid#83390Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only