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Plasmid#160647Purposemodule containing the human codon optimized Cas9 and the BASTA selection markerDepositorInsertBASTA / Cas9
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnos, P35SAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
Danio alpha Ba-crystallin 3kb promoter/AcGFP
Plasmid#98097PurposeaBA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryosDepositorInsertAlpha Ba-crystallin promoter 3 kb fragment, Danio rerio (cryaba Zebrafish)
UseGfp expressingTagsGFPExpressionMutationPromoterDanio alpha Ba-crystallin 3 kb fragment (-3000/-1)Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant SigmaR1-mNeonGreen R119A F191A
Plasmid#226574PurposeEncodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreenDepositorInsertSigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
UseTagsmNeonGreenExpressionMammalianMutationR119A, F191APromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant ss-SigmaR1DTM-mNeonGreen R119A F191A
Plasmid#226576PurposeEncodes mutant siRNA resistant SigmaR1 protein (transmembrane region deletion + substitutions) labeled with mNeonGreenDepositorInsertSigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
UseTagsmNeonGreenExpressionMammalianMutationR119A, F191APromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRIP8b (constant) ion channel scFv [N212/17]
Plasmid#206725PurposeMammalian Expression of TRIP8b (constant) ion channel scFV. Derived from hybridoma N212/17 scFv.DepositorInsertTRIP8b (constant) ion channel (Rattus norvegicus) recombinant scFV (Pex5l Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO–γ-tubulinWT-RNAi-resistant-GFP
Plasmid#205603PurposeTet-inducible expression of WT gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertionDepositorInsertγ-tubulinWT-RNAi-resistant-GFP
UseTagsGFPExpressionMammalianMutationG2228A, C2231T, C2237T, G2240APromoterAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO–γ-tubulinΔGTP-RNAi-resistant-GFP
Plasmid#205604PurposeTet-inducible expression of N229A gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertionDepositorInsertγ-tubulinΔGTP-RNAi-resistant-GFP
UseTagsGFPExpressionMammalianMutationG2228A, C2231T, C2237T, G2240A, N229APromoterAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-EGFP MASTL E167D (siRNA resistant)
Plasmid#191013PurposeExpresses EGFP-tagged MASTL with E167D mutation and resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)DepositorInsertMicrotubule-associated serine/threonine kinase like (MASTL Human)
UseTagsEGFPExpressionMammalianMutationE167DPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pan-Gamma-protocadherin-Constant scFv [N159/5]
Plasmid#190533PurposeMammalian Expression of Pan-Gamma-protocadherin-Constant scFV. Derived from hybridoma N159/5.DepositorInsertPan-Gamma-protocadherin-Constant (Mus musculus) recombinant scFV (Pcdhga1 Mouse)
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
Anti-Pan-Gamma-protocadherin-Constant [N159/5R]
Plasmid#177503PurposeMammalian Expression Plasmid of anti-Pan-Gamma-protocadherin-Constant (Mouse). Derived from hybridoma N159/5.DepositorInsertanti-Pan-Gamma-protocadherin-Constant (Mus musculus) recombinant mouse monoclonal antibody
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27Linear-1, PolyU(0), Hairpin Distance = 0nt
Plasmid#166993PurposeTests for the impact of a synthetic linear tract in conjunction with a hairpin motif on human polymerase III transcription from a U6 promoter.DepositorInsertTermination module:PolyU(0), Hairpin = 0 nt
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
GLN, Linear-3, PolyU(1), Hairpin Distance = 0nt
Plasmid#166986PurposeTests for the impact of 1 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.DepositorInsertTermination module:PolyU(1), Hairpin = 0 nt
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
GLY, Linear-3, PolyU(4), Hairpin Distance = 0nt
Plasmid#166991PurposeTests for the impact of 4 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.DepositorInsertTermination module:PolyU(4), Hairpin = 0 nt
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
GLY, Linear-3, PolyU(1), Hairpin Distance = 0nt
Plasmid#166990PurposeTests for the impact of 1 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.DepositorInsertTermination module:PolyU(1), Hairpin = 0 nt
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
GLN, Linear-3, PolyU(4), Hairpin Distance = 0nt
Plasmid#166987PurposeTests for the impact of 4 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.DepositorInsertTermination module:PolyU(4), Hairpin = 0 nt
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27Linear-2, PolyU(0), Hairpin Distance = 0nt
Plasmid#166995PurposeTests for the impact of a synthetic linear tract in conjunction with a hairpin motif on human polymerase III transcription from a U6 promoter.DepositorInsertTermination module:PolyU(0), Hairpin = 0 nt
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Linear-2_PolyU(1)_HairpinDistance(10)nts_Tornado-Corn
Plasmid#159501PurposeTests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.DepositorInsertTermination Module:Linear-2_PolyU(1)_HairpinDistance(10)nts
UseTagsExpressionMammalianMutationPromoterU6-27Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Linear-2_PolyU(4)_HairpinDistance(10)nts_Tornado-Corn
Plasmid#159502PurposeTests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.DepositorInsertTermination Module:Linear-2_PolyU(4)_HairpinDistance(10)nts
UseTagsExpressionMammalianMutationPromoterU6-27Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Linear-3_PolyU(1)_HairpinDistance(10)nts_Tornado-Corn
Plasmid#159504PurposeTests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.DepositorInsertTermination Module:Linear-3_PolyU(1)_HairpinDistance(10)nts
UseTagsExpressionMammalianMutationPromoterU6-27Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(10)nts_Tornado-Corn
Plasmid#159506PurposeTests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.DepositorInsertTermination Module:Linear-3_PolyU(4)_HairpinDistance(10)nts
UseTagsExpressionMammalianMutationPromoterU6-27Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only