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  6. pAW41

    Plasmid
    #110228
    Purpose
    integration of loxP site a distance upstream of gene start site
    Depositor
    Tony Carr
    Article
    Watson et al Gene. 2008 Jan 15;407(1-2):63-74
    Insert
    ura4+ (ura4 Fission Yeast)
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    See depositor comments below.
    Promoter
    Available since
    Feb. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    110228-plasmid-map-sequence-id-219790

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  8. pSFVCs-lacZ

    Plasmid
    #92076
    Purpose
    This is a high level expression plasmid for production of recombinant genomic SFV RNA encoding a C-beta galactosidase fusion protein.
    Depositor
    Henrik Garoff
    Article
    Sjoberg et al Biotechnology (N Y). 1994 Nov;12(11):112
    Insert
    C-lacZ
    Use
    C-lacz expression plasmid, for run-off transcript…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    92076-plasmid-map-sequence-id-181625

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  15. C078

    Plasmid
    #174347
    Purpose
    Template for making mRNA for overexpressing frog ePAB (C-FLAG) in frog oocytes
    Depositor
    David Bartel
    Article
    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1
    Insert
    ePAB
    Use
    In vitro transcription template
    Tags
    FLAG
    Expression
    Mutation
    13 silent mutations: G243A, A273G, G339A, C345G, …
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    174347-plasmid-map-sequence-id-343308

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  17. pSFV-Helper1

    Plasmid
    #92073
    Purpose
    This is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.
    Depositor
    Henrik Garoff
    Article
    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356
    Insert
    cDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
    Use
    Helper plasmid, for run-off transcription and rna…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92073-plasmid-map-sequence-id-275942

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  18. 1.3xWT HBV-Luciferase

    Plasmid
    #61549
    Purpose
    The 1.3xHBV-Luciferase plasmid to monitor HBV transcription. 1. Luciferase inserted within Core ORF. 2. Luciferase is regulated by EnhII - Core promoter. 3. The functional luciferase ATG is of Core
    Depositor
    Yosef Shaul
    Article
    Doitsh et al Mol Cell Biol. 2004 Feb;24(4):1799-808.
    Insert
    1.3 times of the HBV genome with luciferase within the Core ORF
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Substitution of HBV sequences with luciferase ORF…
    Promoter
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    61549-plasmid-map-sequence-id-212455

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