Addgene

We narrowed to 11,257 results for: pcDNA

Showing: 781 - 800 of 11257 results

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Twitch-4 pcDNA3

Plasmid

#49533

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-4

Use

Tags

ECFP and cpCit174

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 SIRT6_H133Y

Plasmid

#53149

Purpose

expression vector for catalyitc inactive human Sirtuin6

Depositor

Katrin Chua

Article

Michishita et al Nature. 2008 Mar 27;452(7186):492-6. doi

Insert

Sirt6 (SIRT6 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

H133Y, catalytic inactive

Promoter

CMV

Available since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-HA_mouse_Adar2_Tv1

Plasmid

#158110

Purpose

expresses mADAR2 shortest transcript

Depositor

Emanuela Felley-Bosco

Insert

mAdar2 (Adarb1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LumiLuc

Plasmid

#126622

Purpose

An engineered luciferase to enhance the bioluminescence of synthetic analog-8pyDTZ

Depositor

Huiwang Ai

Article

Yeh et al ACS Chem Biol. 2019 Apr 17. doi: 10.1021

Insert

LumiLuc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA_GFP-mTLN1-FL

Plasmid

#164838

Purpose

Expression of GFP tagged FL mouse talin1

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

Insert

TLN1 (Tln1 Mouse)

Use

Tags

His10-GFP

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA_B2AR-i-pep

Plasmid

#47436

Purpose

FRET sensor for beta2-adrenergic receptor

Depositor

Sivaraj Sivaramakrishnan

Article

Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

Insert

beta2-adrenergic receptor (ADRB2 Human)

Use

Tags

10 nm ER/K helix, HA, i-pep, mCerulean, and mCitr…

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Sept. 11, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_LifeAct-iBlueberry

Plasmid

#73176

Purpose

Expressing iBlueberry and mCherry in mammalian cells

Depositor

Xiaokun Shu

Article

Yu et al Protein Sci. 2016 Feb;25(2):308-15. doi:

Insert

LifeAct-iBlueberry

Use

Tags

Expression

Mammalian

Mutation

mutation E177Q in iBlueberry

Promoter

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/NS1

Plasmid

#120824

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

NS1 ZIKV

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ZnRed

Plasmid

#170782

Purpose

cytosolic ZnRed zinc sensor with 166 nM and 20 uM Kd

Depositor

Huiwang Ai

Article

Chen et al Anal Chem. 2016 Sep 20;88(18):9029-36. d

Insert

ZnRed

Use

Tags

Expression

Mammalian

Mutation

K255N

Promoter

CMV

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2(WT)

Plasmid

#145147

Purpose

Mammalian expression plasmid for soluble ACE2 (protease domain)

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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Keratin 8 (pcDNA3)

Plasmid

#18063

Depositor

M Bishr Omary

Article

Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

Insert

Keratin 8 (KRT8 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_BA.2.12.1

Plasmid

#186809

Purpose

pseudovirus plasmid that expresses spike of BA.2.12.1

Depositor

David Nemazee

Article

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

Spike (BA.2.12.1) (S SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

L452Q, S704L on the basis of BA.2, c-terminal 18 …

Promoter

CMV

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TrxRFP2

Plasmid

#172322

Purpose

an improved fluorescent biosensor for thioredoxin redox

Depositor

Huiwang Ai

Article

Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.

Insert

TrxRFP2, an improved version from TrxRFP1

Use

Tags

His-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-D2s-L-V1

Plasmid

#19967

Depositor

Jonathan Javitch

Article

Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

Insert

D2 short variant-N terminal Venus (DRD2 Human, Aequorea Victoria)

Use

Tags

Flag and N terminal Venus

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2009

Availability

Academic Institutions and Nonprofits only
Cav2.1 HA pcDNA3

Plasmid

#206069

Purpose

expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

Insert

cacna1a (Cacna1a Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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HA-EglN2-pcDNA3

Plasmid

#18961

Depositor

William Kaelin

Article

Lee et al Cancer Cell. 2005 Aug . 8(2):155-67.

Insert

Egg laying Nine - 2 (EGLN2 Human)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Promoter

Available since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-ADH

Plasmid

#11610

Depositor

Steven Johnson

Insert

aldehyde dehydrogenase (ALDH1A1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K412I

Promoter

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) mFis1 V1

Plasmid

#44600

Depositor

David Chan

Article

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

mFis1 variant 1 (Fis1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA4-ATR-C

Plasmid

#53769

Purpose

mammalian expression of flag-tagged ATR C-terminal fragment

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Proc Natl Acad Sci U S A. 2002 May 14;99

Insert

ATR (ATR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

C-terminal domain, aa 1398-2644*

Promoter

CMV

Available since

July 8, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 KSR2

Plasmid

#25968

Depositor

Rob Lewis

Article

Costanzo-Garvey et al Cell Metab. 2009 Nov . 10(5):366-78.

Insert

Kinase suppressor of Ras 2 (Ksr2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only