Addgene

Showing: 781 - 800 of 3060 results

Results per page:

Sort results by:

pEGFP-mSPDEF deltaN5

Plasmid

#100865

Purpose

Mammalian expression of mouse SPDEF truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

Spdef (Spdef Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

truncated protein 100-325aa

Promoter

CMV

Available since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-mSPDEF deltaN4

Plasmid

#100864

Purpose

Mammalian expression of mouse SPDEF truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

Spdef (Spdef Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

truncated protein 85-325aa

Promoter

CMV

Available since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-mSPDEF deltaN3

Plasmid

#100863

Purpose

Mammalian expression of mouse SPDEF truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

Spdef (Spdef Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

truncated protein 70-325aa

Promoter

CMV

Available since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-mSPDEF deltaN2

Plasmid

#100862

Purpose

Mammalian expression of mouse SPDEF truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

Spdef (Spdef Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

truncated protein 55-325aa

Promoter

CMV

Available since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N2-RIPK3 V460P

Plasmid

#78826

Purpose

To detect localization of RIPK3 V460P protein in live mammalian cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

V460P

Promoter

CMV

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only
KHLH31 pEGFP-N2 (1089)

Plasmid

#62032

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

KHLH31

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
NET33 pEGFP-N2 (594)

Plasmid

#61987

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET33 (SCARA5 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-hRIP1-FL I541P

Plasmid

#61381

Purpose

Expresses Human full length RIPK1 with I541P mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP1with I541P mutation (RIPK1 Human)

Use

Tags

EGFP and HA tag

Expression

Mammalian

Mutation

changed I541 to P

Promoter

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp4-a I18A

Plasmid

#40043

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a I18A (SYTL4 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Isoleucine 18 to Alanine

Promoter

CMV promoter

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp4-a linker

Plasmid

#40040

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a linker (SYTL4 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

linker domain (144-353)

Promoter

cmv

Available since

Oct. 17, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-N1 hMOG-alpha1-EGFP

Plasmid

#126463

Purpose

Expression a human MOG (isoform alpha1) EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Mader et al J Neuroinflammation. 2011 Dec 28;8:184.

Insert

Myelin oligodendrocyte glycoprotein (MOG Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-phSyn-flex-SypEGFP

Plasmid

#153203

Purpose

Can be used to generate AAV virus that will mark the presynaptic terminal (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

Depositor

Lynette Lim

Insert

synaptophysin-EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

rat synapsin

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1 Lifeact-EGFP-2XNLS

Plasmid

#58467

Purpose

Expresses a nuclear-targeted actin filament reporter, consisting of the peptide Lifeact, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

Inserts

Lifeact
2X NLS with 2X stop codons

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

Plasmid

#196702

Purpose

Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

Depositor

Karlene Cimprich

Article

Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

Insert

human RNaseH1 wild type

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1 hMOG-beta1-EGFP

Plasmid

#160978

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform beta1 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta1 (MOG Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-MRLC1 T18D, S19D

Plasmid

#35682

Depositor

Tom Egelhoff

Article

Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

Insert

MRLC1 (MYL9 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T18D and S19D

Promoter

CMV

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-FUS/TLS-FLAGC

Plasmid

#60362

Purpose

Plasmid for expression of FLAG-GFP tagged human FUS/TLS (C-terminal tag). Confers resistance to G418.

Depositor

Patrick Calsou, Sébastien Britton

Article

Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

Insert

FUS (FUS Human)

Use

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP Cep170 (Nigg PID200)

Plasmid

#41150

Depositor

Erich Nigg

Article

Guarguaglini et al Mol Biol Cell. 2005 Mar;16(3):1095-107.

Insert

Cep170 (CEP170 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV, T7

Available since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1-GFP-SF3B6-FLAG

Plasmid

#86870

Purpose

To express SF3B6 with a GFP and FLAG tags at N- and C-terminus, respectively.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

SF3B6 (SF3B6 Human)

Use

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP_mNG2(11)_Clathrin light chain

Plasmid

#82608

Purpose

Expresses mNG2(11) tagged clathrin light chain in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

mNG2(11)_Clathrin light chain

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge