Addgene

We narrowed to 4,874 results for: hwa

Showing: 781 - 800 of 4874 results

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pAW8ENdeI-c3HA

Plasmid

#48989

Purpose

for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

3XHA tag

Expression

Mutation

Promoter

Available since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-3XDSR

Plasmid

#48976

Purpose

for Cre-lox cassette exchange of untagged gene sequences together with 3 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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p201N 1509

Plasmid

#55768

Purpose

Contains soybean miRNA miR1509 recognition sequence (CAACCTTGATTTCCTTGATTAA) to produce siRNAs from 3' target sequences and induce RNA silencing

Depositor

Wayne Parrott

Article

Jacobs et al Plant Biotechnol J. 2015 Mar 27. doi: 10

Type

Empty backbone

Use

RNAi

Tags

Expression

Mutation

Promoter

GmUbi

Available since

Sept. 8, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pRGM10

Plasmid

#48790

Purpose

I-SceI DSB repair reporter cassette

Depositor

Michael Glickman

Article

Gupta et al Mol Microbiol. 2011 Jan;79(2):316-30. do

Inserts

lacZ(I-SceI)
lacZ-deltaN

Use

Dsb repair reporter

Tags

Expression

Mutation

Promoter

Available since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pGH1173

Plasmid

#223677

Purpose

pEF-HA-PYL1-NLS-CloningSite-mCherry-puroR

Depositor

Gaelen Hess

Article

Liila-Fogarty et al ACS Synth Biol. 2024 Oct 19. doi: 10.102

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD33(A-H*HA-N)

Plasmid

#187086

Purpose

Medium expression of Complex I with subunits N and H -HA tagged

Depositor

Steven Vik

Article

Zhang et al BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2

Insert

nuoA-N

Use

Tags

HA tags nuoN, nuoH

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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sCatch-GFP

Plasmid

#186902

Purpose

Cell-free expression of soluble protein sfGFP-SpyCatcher-sfCherry1-10

Depositor

Allen Liu, Taekjip Ha

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

sfGFP-SpyCatcher003-sfCherry1-10

Use

Synthetic Biology

Tags

sfGFP

Expression

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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PAJ389

Plasmid

#104147

Purpose

dCas9 plasmid expressing 2A sRNA trigger

Depositor

Alfonso Jaramillo

Article

Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pLtetO-1

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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PAJ390

Plasmid

#104148

Purpose

dCas9 plasmid expressing 3A sRNA trigger

Depositor

Alfonso Jaramillo

Article

Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pLtetO-1

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pWDY1211

Plasmid

#138591

Purpose

Expresses NatL2 in E.coli

Depositor

James Naismith

Article

Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

Insert

phenylacetate-coenzyme A ligase

Use

Tags

6-His

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2(+)-XSmurf1C699A

Plasmid

#117245

Purpose

mRNA synthesis

Depositor

Gerald Thomsen

Article

Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

Insert

Smurf1-C699A mutant

Use

Xenopus expression

Tags

Expression

Mutation

mutated cysteine 699 to alanine

Promoter

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pHBSTC146

Plasmid

#124357

Purpose

Plasmid expressing inactive N- and C-terminal split fragments of gp41-1 intein inserted into CcdA antitoxin

Depositor

Hideo Iwai

Article

Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb

Insert

gp41-1 N-intein (C1G) and C-intein (bicistronic)

Use

Tags

CcdA-C (SFAD)

Expression

Bacterial

Mutation

C1G

Promoter

STC

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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PAJ379

Plasmid

#104143

Purpose

Expresses 3A igRNA and sfGFP

Depositor

Alfonso Jaramillo

Article

Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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PAJ407

Plasmid

#104144

Purpose

Expresses 3A_v9 igRNA and sfGFP

Depositor

Alfonso Jaramillo

Article

Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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PAJ426

Plasmid

#104145

Purpose

Expresses HIV3 igRNA and sfGFP

Depositor

Alfonso Jaramillo

Article

Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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PAJ469

Plasmid

#104146

Purpose

Expresses 3A igRNA and Hybrid-promoter sfGFP

Depositor

Alfonso Jaramillo

Article

Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pSB58 (Prp16)

Plasmid

#111282

Purpose

Prp16 in pSE358(Trp)

Depositor

Christine Guthrie

Article

Burgess et al Cell. 1993 Jul 2;73(7):1377-91.

Insert

PRP16

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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P0050::kaiBC

Plasmid

#101828

Purpose

drives expression of kaiBC from an rpaA-independent promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

kanamycin casette and p0050 promoter

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Ptrc::glgP_gap1

Plasmid

#101845

Purpose

Replaces native promoter of glgP_gap1 with Ptrc promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

CmR casette followed by ptrc promoter

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Ptrc::zwf_fbp_opcA

Plasmid

#101846

Purpose

Replaces native promoter of zwf_fbp_opcA with Ptrc promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

sp/st resistance casette followed by ptrc promoter

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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