We narrowed to 3,744 results for: golden gate

1. pLPC51

   Plasmid

   #209990

   Purpose

   Contains Level 0 Part: 5' Connector (5C1CSN) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher, Georg Fritz

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   5' Connector (5C1CSN)
   

   Expression

   Bacterial

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDM098

   Plasmid

   #216818

   Purpose

   Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for C-terminal tagging.

   Depositor

   Ken Sawin

   Article

   Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

   Insert

   mStayGold
   

   Tags

   FLAG-(SGGS)x2-XTEN16-(GGGGS)x3

   Expression

   Bacterial

   Mutation

   Aspergillus nidulans codon-adjusted

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMA-SpCas9-g10

   Plasmid

   #80793

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 10/20/30 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMA-SpCas9-g9

   Plasmid

   #80792

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 9/19/29 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMA-SpCas9-g8

   Plasmid

   #80791

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEM-T Easy-AtpU6-26

   Plasmid

   #160218

   Purpose

   AtpU6-26 Golden Gate level 0 piece to express gRNAs

   Depositor

   Matias Kirst

   Article

   Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

   Insert

   pAtU6-26 promoter
   

   Use

   Golden gate level 0 piece to express grnas

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. BB2_EF

   Plasmid

   #98538

   Purpose

   empty BB2 for transcription unit cloning

   Depositor

   Michael Sauer, Diethard Mattanovich, Brigitte Gasser

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBT100-neo

   Plasmid

   #123137

   Purpose

   Golden Gate donor vector for the assembly of EGFP-based mammalian recombinase reporters.

   Depositor

   David Liu

   Article

   Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

   Insert

   Neomycin, poly-A terminator
   

   Use

   Synthetic Biology; Golden gate donor vector

   Promoter

   none

   Available Since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. HSA_3b*_AP

   Plasmid

   #238899

   Purpose

   Plasmid insert contains a gene sequence for Human serum albumin for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

   Depositor

   Joseph Brock

   Article

   Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

   Insert

   Recombinant Human Serum Albumin (HSA) 3b*
   

   Use

   Synthetic Biology

   Available Since

   Sept. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. C-term_TEV_aMFx1 (4a)

    Plasmid

    #238903

    Purpose

    Plasmid insert contains the aMFx1 C-terminal tag sequence with the TEV cleavage site for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    C-term_TEV_aMFx1
    

    Use

    Synthetic Biology

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. C-term_C-myc (4a)

    Plasmid

    #238907

    Purpose

    Plasmid insert contains the C-myc C-terminal tag sequence for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    C-term_C-myc
    

    Use

    Synthetic Biology

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. SP_H3

    Plasmid

    #228189

    Purpose

    Plasmid contains the secretion signal peptide sequence of the yeast CIS3 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    sp_P47001_CIS3_YEAST:0-21
    

    Use

    Synthetic Biology

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. SP_H4

    Plasmid

    #228190

    Purpose

    Plasmid contains the secretion signal peptide sequence of the human CCL8 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    sp_P80075_CCL8_HUMAN:0-23
    

    Use

    Synthetic Biology

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. SP_H5

    Plasmid

    #228191

    Purpose

    Plasmid contains the secretion signal peptide sequence of the human SDC4 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    sp_P31431_SDC4_HUMAN:0-20
    

    Use

    Synthetic Biology

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. SP_H6

    Plasmid

    #228192

    Purpose

    Plasmid contains the secretion signal peptide sequence of the human BPIB4 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    sp_P59827_BPIB4_HUMAN:0-17
    

    Use

    Synthetic Biology

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. SP_F2

    Plasmid

    #228176

    Purpose

    Plasmid contains the secretion signal peptide sequence of the yeast EXG1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    sp_P23776_EXG1_YEAST:0-19
    

    Use

    Synthetic Biology

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. SP_F3

    Plasmid

    #228177

    Purpose

    Plasmid contains the secretion signal peptide sequence of the yeast ECM33 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    sp_B5VE42_ECM33_YEAS6:0-19
    

    Use

    Synthetic Biology

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. SP_F4

    Plasmid

    #228178

    Purpose

    Plasmid contains the secretion signal peptide sequence of the human CCL2 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    sp_P13500_CCL2_HUMAN:0-23
    

    Use

    Synthetic Biology

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. SP_F5

    Plasmid

    #228179

    Purpose

    Plasmid contains the secretion signal peptide sequence of the human MMP11 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    sp_P24347_MMP11_HUMAN:0-33
    

    Use

    Synthetic Biology

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. SP_F6

    Plasmid

    #228180

    Purpose

    Plasmid contains the secretion signal peptide sequence of the human PDGFC gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    sp_Q9NRA1_PDGFC_HUMAN:0-22
    

    Use

    Synthetic Biology

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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