Addgene

Showing: 781 - 800 of 3890 results

Results per page:

Sort results by:

pBAD02_CBASS_Escherichia_coli_703128

Plasmid

#224394

Purpose

For expression of the type I CBASS system encoded by Escherichia coli 703128

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

EcCdnD_4TM

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD02_CBASS_Escherichia_coli_BWH59

Plasmid

#224393

Purpose

For expression of the type I CBASS system encoded by Escherichia coli BWH59

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

2TM_EcCdnD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD02_CBASS_Rhodanobacter_denitrificans

Plasmid

#224391

Purpose

For expression of the type I CBASS system encoded by Rhodanobacter denitrificans

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

Phospholipase_RdCdnA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Halo-MDC1 PST deletion

Plasmid

#207109

Purpose

pHTN HaloTag CMV-neo (Promega; #G7721) based vector to express HaloTag-MDC1 with a deletion of the PST repeat region in human cells.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

HaloTag-MDC1 PST deletion

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Halo-MDC1 BRCT domain deletion

Plasmid

#207110

Purpose

pHTN HaloTag CMV-neo (Promega; #G7721) based vector to express HaloTag-MDC1 with a deletion of the BRCT domain in human cells.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

HaloTag-MDC1 BRCT domain deletion

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ULK1-Halo HRD

Plasmid

#207548

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the C-terminus of the endogenous ULK1 locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag followed by a PolyA signal and PuroR cassette flanked by human ULK1 locus sequences

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG NC

Plasmid

#214675

Purpose

Negative control plasmid for splicing reporters consisting of MCP fused FLAG epitope tags

Depositor

Eugene Yeo

Article

Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

FLAG

Use

Tags

MCP and V5

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKD286

Plasmid

#125074

Purpose

Expression of SO_4622 under Ptac

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_4622 (SO_4622 )
lacI (lacI )

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

S286L- Please see depositor comments below

Promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKD276

Plasmid

#125069

Purpose

Expression of SO_0621 under Ptac

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_0621 (SO_0621 )
lacI (lacI )

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

S286L- Please see depositor comments below

Promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKD250.2

Plasmid

#125077

Purpose

Expression of torR under PLtetO-1, output promoter PtorCAD129

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

torR (torR )
sfgfp
tetR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSR300.3

Plasmid

#125079

Purpose

Expression of uhpA under PLtetO-1, output Promoter PuhpT99

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

uhpA (uhpA )
sfgfp
tetR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1291 TDP43 RRM-GFP G368W

Plasmid

#107837

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

G368W mutation in LLPS reporter

Promoter

Available since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS934 TDP43 RRM-GFP M-V

Plasmid

#107801

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

All methionine to valine in LLPS reporter

Promoter

Available since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS935 TDP43 RRM-GFP M-S

Plasmid

#107800

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

All methionine to serine in LLPS reporter

Promoter

Available since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1052 TDP43 RRM-GFP R-K

Plasmid

#107856

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

All arginine to lysine in LLPS reporter

Promoter

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1279 TDP43 RRM-GFP G348V

Plasmid

#107831

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

G348V mutation in LLPS reporter

Promoter

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1280 TDP43 RRM-GFP G348F

Plasmid

#107830

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

G348F mutation in LLPS reporter

Promoter

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS968 TDP43 RRM-GFP G309S

Plasmid

#107824

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

G309S mutation in LLPS reporter

Promoter

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS966 TDP43 RRM-GFP G309F

Plasmid

#107822

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

G309F mutation in LLPS reporter

Promoter

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1256 TDP43 RRM-GFP W385G

Plasmid

#107807

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

W385G mutation in LLPS reporter

Promoter

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge