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Plasmid#50738PurposeExpresses redCALWY-4 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.DepositorInsertgene for redCALWY-4 Zn2+ sensor
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGen2.1-redCALWY-1
Plasmid#50739PurposeExpresses redCALWY-1 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.DepositorInsertgene for redCALWY-1 Zn2+ sensor
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-p52-HDM2_sensor
Plasmid#127184PurposeEukaryotic expression of the fusion protein used for drug diruption the p53-HDM2 interactionDepositorInsertp53-YPeT-Pro15-GGS2-Pro15-CFP-HDM2
UseTagsHisTag and StrepTagExpressionMammalianMutationPromoterCMV-TetO2Available sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-SNAP-HCA_sensor
Plasmid#127181PurposeBacterial expression of the fusion protein used for sulfa-drugs sensingDepositorInsertSNAP-YPeT-Pro15-GGS2-Pro15-CFP-HCA
UseTagsHisTag and StrepTagExpressionBacterialMutationPromoterT7Available sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLBT0180
Plasmid#172814PurposeLevel 1 module containing TP-CFP_P4 FDepositorInsertp35S-5UTMV-CTPSSU-CFP-3U+TER35S
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-tdTomato
Plasmid#83029Purposeexpresses tdTomato fluorescent protein under the CAG promoterDepositorInserttdTomato
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMesh1*
Plasmid#175591PurposeLac inducible, AMP Resistant, fluor labelled (CFP) gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.DepositorInsertMesh1 (Mesh1 Fly)
UseTagsFused with a flexible GS linker to ceruleanExpressionBacterialMutationCodon optimized for translation in E.coli. AGGAGG…PromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSP1830
Plasmid#70708PurposeHuman expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAGDepositorInsertmammalian codon-optimized KKH variant Staphylococcus aureus Cas9
UseCRISPRTagsNLS-3xFLAGExpressionMammalianMutationE782K, N968K and R1015H in SaCas9PromoterCAGAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
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BPK2139
Plasmid#65776PurposeHuman expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAGDepositorInsertmammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
UseCRISPRTagsNLS-3xFLAGExpressionMammalianMutationPromoterCAGAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
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MSP1594
Plasmid#65775PurposeHuman expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLSDepositorInsertmammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS
UseCRISPRTagsNLSExpressionMammalianMutationPromoterCAGAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
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MacroH2A.2-c002
Plasmid#173089PurposeExpression of recombinant fusion protein in E. coliDepositorInsertMACROH2A2 (MACROH2A2 Human)
UseTagsmCeruleanExpressionBacterialMutationK167-K372PromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
AF1521-c002
Plasmid#173109PurposeExpression of recombinant fusion protein in E. coliDepositorInsertAF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))
UseTagsmCeruleanExpressionBacterialMutationKH-M1-L192PromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pLBM0090
Plasmid#172824PurposeLevel 2 module containing mCHERRY-nVENUS HSP21-cVENUSDepositorInsert[35S-P19][35S-CTPSSU-mHSP21-nVENUS][35S-CTPSSU-mHSP21-X3FLAGcVENUS][35S-SP_SV40-CFP]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
JEH127
Plasmid#179299PurposeExpresses dCas9 fused to VPRDepositorInsertdCas9-VPR
UseTagsExpressionMammalianMutationD10A, H840A (catalytically inactive Cas9)PromoterCAGAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
BPK1160
Plasmid#179298PurposeExpresses dCas9 fused to P65DepositorInsertdCas9-p65
UseTagsExpressionMammalianMutationD10A, H840A (catalytically inactive Cas9)PromoterCAGAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-DKAR-Nuc
Plasmid#45499DepositorInsertDKAR-Nuc
UseTagsCFP, NLS, and YFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only