Addgene

We narrowed to 1,114 results for: cbs

Showing: 781 - 800 of 1114 results

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HLH Clock Pas 1-401 VenN

Plasmid

#47353

Purpose

Clock fragment cloned and tagged with VenN

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Use

Tags

Venus aa 1-155

Expression

Mammalian

Mutation

aa 1-401

Promoter

CMV

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pAG182 FGFR1 3'UTR mut

Plasmid

#12009

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N15 3'UTR mut (FGFR1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Promoter

Available since

June 8, 2006

Availability

Academic Institutions and Nonprofits only
HLH Bmal Pas 1-465-VenC I317D

Plasmid

#47367

Purpose

Bmal fragment cloned with Venus tag used for mutation

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Use

Tags

Venus aa 156-239

Expression

Mammalian

Mutation

aa 1-465, I317D

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
HLH Clock Pas 1-401 VenN L74E

Plasmid

#47355

Purpose

Clock fragment mutatation tagged with VenN used for BiFC

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Use

Tags

Venus aa 1-155

Expression

Mammalian

Mutation

aa 1-401, L74E

Promoter

CMV

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
attB-GFP-Tet1d834-1053-Poly(A)

Plasmid

#65561

Purpose

Plasmid for Bxb1-mediated recombination of the GFP-Tet1d834-1053 cDNA into a MIN-tagged locus

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Tet1 (Tet1 Mouse)

Use

Mouse Targeting; Bxb1

Tags

GFP

Expression

Mammalian

Mutation

del(834-1053)

Promoter

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pfast bac-flag Fbxl3

Plasmid

#51894

Purpose

Backbone plasmid is pfastBacHTa from invitrogen

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

Fbxl3 (Fbxl21 Mouse)

Use

Tags

FLAG

Expression

Mutation

Promoter

polyhedrin promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
CMV-FFluc-GPD1L-3'UTR WT

Plasmid

#32488

Depositor

Pere Puigserver

Article

Kelly et al Mol Cell Biol. 2011 Jul;31(13):2696-706.

Insert

GPD1L 3'UTR (Gpd1l Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only
HLH Bmal Pas 1-465-VenC

Plasmid

#47364

Purpose

Bmal fragment cloned with Venus tag and used for BiFC

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Use

Tags

Venus aa 156-239

Expression

Mammalian

Mutation

aa 1-465

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
CMV-FFluc-GPD1L-3'UTR Full mut

Plasmid

#32489

Depositor

Pere Puigserver

Article

Kelly et al Mol Cell Biol. 2011 Jul;31(13):2696-706.

Insert

GPD1L 3'UTR (Gpd1l Mouse)

Use

Tags

Luciferase

Expression

Mammalian

Mutation

Mutated in the entire miR-210 binding region

Promoter

CMV

Available since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only
CMV-FFluc-GPD1L-3'UTR Seed Mut

Plasmid

#32490

Depositor

Pere Puigserver

Article

Kelly et al Mol Cell Biol. 2011 Jul;31(13):2696-706.

Insert

GPD1L 3'UTR (Gpd1l Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only
TLCV2-Scal-T2A-EGFP-Puro

Plasmid

#234949

Purpose

Doxycycline-inducible mitochondria-localized ScaI expressing plasmid

Depositor

Agnel Sfeir

Article

Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

ScaI restriction enzyme with N-terminal COX8 mitochondrial localization signal

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

tight TRE promoter

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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TLCV2-ApaLI-T2A-EGFP-Puro

Plasmid

#234948

Purpose

Doxycycline-inducible mitochondria-localized ApaLI expressing plasmid

Depositor

Agnel Sfeir

Article

Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

ApaLI restriction enzyme with N-terminal COX8 mitochondrial localization signal

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

tight TRE promoter

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-SCN8A-Variant-3-IRES-mScarlet

Plasmid

#209411

Purpose

Expression of wild-type adult isoform of human SCN8A.

Depositor

Al George

Article

Vanoye et al JCI Insight. 2024 May 21;9(12):e177530.

Insert

SCN8A Variant 3, stabilized (SCN8A Human)

Use

Tags

Expression

Mammalian

Mutation

Modifed human HSPA5 intron 4 inserted after bp237…

Promoter

CMV

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCDF-rM1PYK

Plasmid

#220232

Purpose

Inducible expression of rabbit muscle pyruvate kinase isoform 1

Depositor

Liskin Swint-Kruse, Aron Fenton

Article

Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

Insert

Rabbit muscle pyruvate kinase (PKM )

Use

Tags

6xHis-MBP-SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCDF-hLPYK

Plasmid

#220231

Purpose

Inducible expression of human liver pyruvate kinase

Depositor

Liskin Swint-Kruse, Aron Fenton

Article

Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

Insert

Human liver pyruvate kinase (PKLR Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pET15-ZmPYK

Plasmid

#220230

Purpose

Inducible expression of Zymomonas mobilis pyruvate kinase

Depositor

Liskin Swint-Kruse, Aron Fenton

Article

Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

Insert

Zymomonas mobilis pyruvate kinase (pyk, ZMO0152 Zymomonas mobilis)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speGpromoter_SAT1 (human)

Plasmid

#220477

Purpose

heterologous expression of SSAT1 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

SSAT1 (SAT1 Human)

Use

Tags

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pILGFP4QE2Crimson

Plasmid

#218280

Purpose

Introduction of a bicistronic expression cassette of yEGFP and E2Crimson under the control of the SkGAL2 promoter

Depositor

Claudia Vickers

Article

Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

Insert

pURA3>KlURA3>tKlURA3-pSkGAL2>yEGFP>ERBV1.2A>E2Crimson>tURA3

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pIRGAL4A

Plasmid

#218277

Purpose

Introduction of a LowTempGAL Turbo module (GAL4) to accelerate induction of GAL promoter induction

Depositor

Claudia Vickers

Article

Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

Insert

fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-fcy1(454-778)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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UNK_6xZNF_pGEX

Plasmid

#215703

Purpose

Recombinant expression of UNK with dual-affinity tag

Depositor

Christopher Burge, Daniel Dominguez

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

UNK (UNK Human)

Use

Tags

GST and SBP

Expression

Bacterial

Mutation

Promoter

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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