Skip to main content
Addgene
Showing: 781 - 800 of 933 results

  3. PB-TO-hNIL

    Plasmid
    #172113
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Article
    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1
    Inserts
    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and TRE3G
    Availability
    Academic Institutions and Nonprofits only
    172113-plasmid-map-sequence-id-359519

    Enlarge

  10. pTRIP TRE BI_GFP10-14-SEPT7 Gmut2_GFP11-14-SEPT7 Gmut2

    Plasmid
    #180353
    Purpose
    mammalian co-expression of human SEPT7 Gmut2 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11
    Depositor
    Manos Mavrakis
    Article
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Inserts
    SEPTIN7 (SEPTIN7 Human)
    SEPTIN7 (SEPTIN7 Human)
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    SEPT7 H279D
    Promoter
    bidirectional TRE
    Availability
    Academic Institutions and Nonprofits only
    180353-plasmid-map-sequence-id-357939

    Enlarge

  13. pUA97

    Plasmid
    #69340
    Purpose
    It encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategy
    Depositor
    Oliver Hobert
    Insert
    ceBFP SEC 3xFlag
    Use
    Tags
    3xFlag and ceBFP (mTagBFP)
    Expression
    Worm
    Mutation
    sqt-1(e1350)
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    69340-plasmid-map-sequence-id-133241

    Enlarge

  14. L1T11 - T2BP/p35S (BFP, mCherry, NeonGreen)

    Plasmid
    #219688
    Purpose
    Level 1 History-dependent target, switch Bxb1 then PhiC31, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Jennifer Nemhauser
    Insert
    T2BP/p35S (BFP, mCherry, NeonGreen)
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    219688-plasmid-map-sequence-id-435723

    Enlarge

Showing: 781 - 800 of 933 results