Showing: 781 - 800 of 2422 results
-
Plasmid#213009PurposeA lentiviral vector used to create stable cell lines expressing the ABE8e-SpRY base editor and BFPDepositorInsertABE8e-SpRY-D10A
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A nickase variant of SpRYPromoterEf-1aAvailabilityAcademic Institutions and Nonprofits only -
#2.4 pre-G-SynapShot-TagBFP
Plasmid#214721PurposeExpresses green #2.4 pre-SynapShot and TagBFPDepositorInsert#2.4 pre-G-SynapShot
UseAAVTagsTagBFPExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-FLEX-H2BmTagBFP2-oG
Plasmid#217976PurposeAAV transfer plasmid to express H2B-BFP and codon-optimized Rabies G proteinDepositorInsertsH2BmTagBFP2
oG
UseAAVTagsmTAGBFP2ExpressionMutationPromoterhSynAvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-C1-SACM1LdeltaTMD-C389S-Fis1tail
Plasmid#220079PurposeSAC1 with a C389S mutation in it with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Blue FluorophoreDepositorInsertSACM1l-C389S-Fis1tail (SACM1L Human)
UseTagsExpressionMammalianMutationC389SdeltaTMDPromoterAvailabilityAcademic Institutions and Nonprofits only -
gRNA-CasRx_SD1-pSV40-TagBFP
Plasmid#221004PurposeTransiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.DepositorInsertCasRx 30nt processed direct repeat with SD1 mutation
UseCRISPRTagsExpressionMammalianMutationDR1 A>TPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pME-dCas9-BFP-KRAB
Plasmid#221502PurposeCRISPRi machinery fish codon optimizedDepositorInsertdCas9-tagBFP-KRAB
UseFish expressionTagsExpressionMutationD10A and H839A point mutationsPromoterAvailabilityAcademic Institutions and Nonprofits only -
pUbi_dCas9-BFP-KRAB-gRNA
Plasmid#221503PurposeCRISPRi machinery fish codon optimized expressing plasmidDepositorInsertdCas9-tagBFP-KRAB
UseFish expressionTagsExpressionMutationD10A and H839A point mutationsPromoterZebrafish Ubiquitin promoterAvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 mTagBFP2 rat DJ-1 shRNA
Plasmid#222870PurposeExpresses mTagBFP2 along with an shRNA against rat DJ-1DepositorInsertDJ-1 shRNA (Park7 Rat)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP
Plasmid#60903PurposeExpressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activationDepositorInsertCas9 dead
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCCL-PGK-SPdCas9-BFP-DNMT1
Plasmid#66818PurposedCas9 fused to BFP and the human DNMT1 catalytic domainDepositorInsertdCas9-BFP-DNMT1, catalytic domain (DNMT1 )
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCCL-PGK-SPdCas9-BFP-DNMT3A
Plasmid#66819PurposedCas9 fused to BFP and the human DNMT3A catalytic domainDepositorInsertdSpCas9-BFP-DNMT3A, catalytic domain of DNMT3A
UseTagsdSpCas9-BFP-DNMT3AExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCCL-PGK-SPdCas9-BFP-DNMT3B
Plasmid#66820PurposedCas9 fused to BFP and the human DNMT3B catalytic domainDepositorInsertdSpCas9-BFP-DNMT3B, catalytic domain of DNMT3B
UseTagsdSpCas9-BFP-DNMT3BExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCCL-PGK-SPdCas9-BFP-EGFP
Plasmid#66821PurposedCas9 fused to BFP and EGFPDepositorInsertdSpCas9-BFP-EGFP
UseTagsdSpCas9-BFP-EGFPExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
hGFAP-NLS-HA-Dre-P2A-BFP
Plasmid#51277PurposeCo-expression of the site-specific recombinase Dre and BFP under the control of the hGFAP promoterDepositorInsertNLS-HA-Dre-P2A-BFP
UseTagsExpressionMutationPromoterhGFAPAvailabilityAcademic Institutions and Nonprofits only -
pAd/CMV/PBX1-IRES-nEBFP2
Plasmid#29755DepositorInsertPre-B-cell leukemia transcription factor 1 (PBX1 Human)
UseTagsEBFP2, IRES, and Nuclear localization sequenceExpressionMammalianMutationNo mutations; EBFP2 is nuclear localized; PBX1 an…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono3
Plasmid#73542PurposeExpression of sgRNA targeting LINE-1 and TagBFPDepositorInsertLINE-1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6AvailabilityAcademic Institutions and Nonprofits only -
pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono1
Plasmid#73543PurposeExpression of sgRNA targeting LINE-1 and TagBFPDepositorInsertLINE-1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6AvailabilityAcademic Institutions and Nonprofits only -
pME-loxP-mTagBFP2-stop-pA-loxP
Plasmid#75158PurposeFloxed TagBFP2 (blue) fluorophore with stop codon for inducible lines.DepositorInsertloxP-mTagBFP2-stop-pA-loxP
UseMiddle entry vector for multisite gateway three f…TagsExpressionMutationPromotern/aAvailabilityAcademic Institutions and Nonprofits only -
pDonor-tBFP-NLS-Neo (Universal)
Plasmid#80767PurposeUniversal donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.DepositorInsertPITCh-gRNA#3 targeting sequence (GCATCGTACGCGTACGTGTT)
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pHR SFFVp BFP-PIF-SOS2cat
Plasmid#50850PurposeOptogenetic Ras activation system; cytoplasmic componentDepositorInsertsUseLentiviralTagsTagBFPExpressionMutationresidues 566–1049PromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 781 - 800 of 2422 results