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Plasmid#214631Purposebarcoded assay, UAS sensor for split TEV assaysDepositorInsert10x clustered UAS element with barcode 0404
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Myc-FBW7]K404R
Plasmid#200715PurposeThe plasmid expresses Myc-tagged FBW7 human isoform 1 with Lys404 to Arg mutation. Used for overexpression and detection by western blotting.DepositorInsertFBW7 (FBXW7 Human)
UseTagsMycExpressionMammalianMutationFBW7 K404 is mutated to Alanine.PromoterCMVAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Myc-FBW7K404/412/R
Plasmid#200716PurposeThe plasmid expresses Myc-tagged FBW7 human isoform 1 with Lys404 & 412 to Arg mutation. Used for mammalian overexpression and detection by western blotting.DepositorInsertFBW7 (FBXW7 Human)
UseTagsMycExpressionMammalianMutationFBW7 K404 and K412 mutated to Alanines.PromoterCMVAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsDCP2-F404AF407AF409A_V
Plasmid#147793PurposeMammalian Expression of HsDCP2-F404AF407AF409ADepositorInsertHsDCP2-F404AF407AF409A (DCP2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot2-del380-404_T
Plasmid#147624PurposeMammalian Expression of DmNot2-del380-404DepositorInsertDmNot2-del380-404 (Rga Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpH-HsNot10_25-404_Q
Plasmid#147300PurposeBacterial Expression of HsNot10_25-404DepositorInsertHsNot10_25-404 (CNOT10 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpH-HsNot10_221-404_Q
Plasmid#147303PurposeBacterial Expression of HsNot10_221-404DepositorInsertHsNot10_221-404 (CNOT10 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP2-E148QF404AF407AF409A_V
Plasmid#147812PurposeMammalian Expression of HsDCP2-E148QF404AF407AF409ADepositorInsertHsDCP2-E148QF404AF407AF409A (DCP2 Human)
UseTagsExpressionMammalianMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMX-Gal4-TEAD1-H404A
Plasmid#33032DepositorInsertTEAD1 (TEAD1 Human)
UseTagsGal4ExpressionMammalianMutationH404APromoterAvailable sinceMarch 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRK5-Myc-TEAD1-H404A
Plasmid#33046DepositorInsertTEAD1 (TEAD1 Human)
UseTagsMycExpressionMammalianMutationH404APromoterAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
HL 1474 (=HL 862 + pHL 404)
Plasmid#30015DepositorInsertpLtetO-1:MicC, pLlacO-1:hfq, pLtetO-1:ompC::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-XL4 ASXL1 (p.R404X) 3x FLAG
Plasmid#74263PurposeCancer-associated ASXL1 truncation mutant in mammalian expression vectorDepositorInsertASXL1 (ASXL1 Human)
UseTags3X FLAGExpressionMammalianMutationp.R404X truncationPromoterCMVAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y401S/Y404S/Y409S
Plasmid#216409PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y401/Y404/Y409 to serine mutationsDepositorInsertEfg1-ΔDBD-Y401S/Y404S/Y409S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 401, 404 and 409 to Serine, and …PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot2-del380-404_T
Plasmid#147630PurposeMammalian Expression of DmNot2-del380-404DepositorInsertDmNot2-del380-404 (Rga Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot2-del380-404-W507E_S
Plasmid#147488PurposeMammalian Expression of HsNot2-del380-404-W507EDepositorInsertHsNot2-del380-404-W507E (CNOT2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
PF3D7_1404900-COMP-blac-flag-his
Plasmid#111018PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertconserved Plasmodium protein (PF3D7_1404900 Synthetic)
UseTagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot2-del380-404-W507E_S
Plasmid#147499PurposeMammalian Expression of HsNot2-del380-404-W507EDepositorInsertHsNot2-del380-404-W507E (CNOT2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST flag-CAD R1404A
Plasmid#188133PurposeExpresses C-terminal flag-tagged human CAD R1404A in mammalian cellsDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tagExpressionMammalianMutationTCC -> AGT silent mutations at nt439-441 elimi…PromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
AiP1712 - pAAV-AiE2404m-minBG-SYFP2-WPRE-BGHpA
Plasmid#214525PurposeAiE2404m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cellsInsertSYFP2
UseAAVTagsExpressionMutationPromoterminBGAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST flag-CAD R1403A R1404A
Plasmid#188135PurposeExpresses C-terminal flag-tagged human CAD R1403A R1404A in mammalian cellsDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tagExpressionMammalianMutationTCC -> AGT silent mutations at nt439-441 elimi…PromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only