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pRL-TK 4x mut F

Plasmid

#11319

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation F (see paper) on inner 2 binding sites.

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut H

Plasmid

#11321

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation H (see paper) on inner 2 binding sites.

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut GFP

Plasmid

#11323

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 bulged binding sites for CXCR4 siRNA antisense, flanking 2 binding sites for GFP

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRB327

Plasmid

#8668

Depositor

David Botstein

Article

Schatz et al Mol Cell Biol 1986 Nov;6(11):3722-33.

Insert

TUB1 (TUB1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 30, 2005

Availability

Academic Institutions and Nonprofits only
pRB2065

Plasmid

#8669

Depositor

David Botstein

Article

Richards et al Mol Biol Cell 2000 May;11(5):1887-903.

Insert

TUB1 upstream of LEU2 (TUB1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 30, 2005

Availability

Academic Institutions and Nonprofits only
pRB2067

Plasmid

#8670

Depositor

David Botstein

Article

Richards et al Mol Biol Cell 2000 May;11(5):1887-903.

Insert

TUB1 upstream of HIS3 (TUB1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 30, 2005

Availability

Academic Institutions and Nonprofits only
pJBL2191

Plasmid

#71205

Purpose

pbuE STAR Antisense Expression Plasmid

Depositor

Julius Lucks

Article

Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

Insert

pbuE STAR Antisense

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pJBL2184

Plasmid

#71209

Purpose

pbuE Sense Expression Plasmid

Depositor

Julius Lucks

Article

Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

Insert

pbuE Sense

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pJBL1864

Plasmid

#71210

Purpose

Anti-anti Sense Expression Plasmid

Depositor

Julius Lucks

Article

Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

Insert

Anti-anti Sense

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT Draper-GFP

Plasmid

#126653

Purpose

Inducible expression of Draper-GFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT Crk-mCherry

Plasmid

#126659

Purpose

Inducible expression of Crk-mCherry in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Crk

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT vCrk-GFP

Plasmid

#126661

Purpose

Inducible expression of vCrk truncation in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Crk

Use

Tags

Expression

Insect

Mutation

residues 1-182

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT Ptp69D-GFP

Plasmid

#126663

Purpose

Inducible expression of Ptp69D-GFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Ptp69D

Use

Tags

Expression

Insect

Mutation

A53S, K105N, and A98T- please see depositor comme…

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT Shark-mCherry

Plasmid

#126668

Purpose

Inducible expression of Shark-mCherry in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Shark mCherry

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pET His10-Draper INT

Plasmid

#126670

Purpose

Expression of His10-Draper intracellular domain for purification from E. coli

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper cytoplasmic domain

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
LIR-wtloxP-TRE-CAG-Frt-red-puro-3xPA-Frt-GFP(mVenus)-KrasG12D-cMyc-SV40LT-IRES-KRABoff-PA-TRE-loxP257-RIR

Plasmid

#67277

Purpose

inducbile color change and cancer regulation: FlpO recombinase dependent expression of KrasG12D cMyc and SV40 large T antigen with doxycycline inducible downregulation through tetKRAB system.

Depositor

Jannik Elverløv-Jakobsen

Article

Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do

Inserts

first casette contain a red fluorescent protein (katushka) linked to puromycin resitance gene through an E2A site
second cassette contains mVENUS-kras-cmyc-SV40LT-KRABoff

Use

Tags

mTQ2 blue fluorescent and red flourescent gene ka…

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 E2F1 HA(Δ2-126) (HindIII- BamHI- EcoRI)

Plasmid

#70668

Purpose

Human mutant of E2F1 lacking amino acids 2 to 126

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

E2F1 lacking residues 2 to 126 (E2F1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

lacks amino acids 2-126

Promoter

cmv

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only
pT7-EGFP-C1-HsGIGYF2-YYMLtoAAAA_AF

Plasmid

#148691

Purpose

Mammalian Expression of HsGIGYF2-YYMLtoAAAA

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2-YYMLtoAAAA (GIGYF2 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation and one deletion of Q1211 com…

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 E2F1 Flag(Δ193-359) (HindIII- BamHI- EcoRI)

Plasmid

#70667

Purpose

Human mutant of E2F1 lacking amino acids 193-359

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

E2F1 lacking residues 193 to 359 (E2F1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

lacks amino acids 193-359

Promoter

cmv

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 E2F1 Flag(Δ 284−359)(HindIII- BamHI- EcoRI)

Plasmid

#70669

Purpose

Human mutant of E2F1 lacking amino acids 284 to 359

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

E2F1 lacking residues 284 to 359 (E2F1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

lacks amin acids 284 to 359

Promoter

cmv

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only