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Plasmid#146317PurposeMammalian Expression of HsAGO4DepositorInsertHsAGO4 (AGO4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-3xGFP-tDeg
Plasmid#191371PurposeOverexpression of 3xGFP-tDeg in human cellsDepositorInsert3xGFP-tDeg
UseTags3xGFPExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-Halo-tDeg
Plasmid#191370PurposeOverexpression of Halo-tDeg in human cellsDepositorInsertHalo-tDeg
UseTagsHaloExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTW072
Plasmid#190247PurposeT-DNA for arabidopsis transformationDepositorInsertgRNA, Cas9, firefly luciferase, bar
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTW069
Plasmid#190246PurposeT-DNA for arabidopsis transformationDepositorInsertgRNA, Cas9, firefly luciferase, bar
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBA2705
Plasmid#189998PurposeInducible expression of FBP75(1–200)-VhhGFP4, integrate at 177 bp, phleomycin marker (deGradFP for cytoplasmic proteins)DepositorInsertsFBP75
VhhGFP4
UseExpression vector for trypanosoma bruceiTagsExpressionMutationN-terminal 600 bp of FBP75PromoterAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBA2675
Plasmid#189997PurposeInducible expression of FBP75 (1–200)-NLS-VhhGFP4, integrate at 177 bp, phleomycin marker (deGradFP for nuclear proteins)DepositorInsertsFBP75
VhhGFP4
UseExpression vector for trypanosoma bruceiTagsNLSExpressionMutationN-terminal 600 bp of FBP75PromoterAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCJH059_proD_Cas12c_DualRNA-GFP_CAM
Plasmid#183076PurposePlasmid expressing Cas12c tracrRNA and a GFP-targeting pre-crRNA separately.DepositorInsertCas12c tracrRNA and pre-crRNA
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCJH009_proD_Cas12c-sgRNA-GFP_CAM
Plasmid#183070PurposePlasmid expressing Cas12c sgRNA targeting GFP (1) for in vivo interference assay in bacteria.DepositorInsertCas12c single guide RNA targeting GFP
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
NK88
Plasmid#176612PurposeTo manipulate ligand spacing.DepositorInsertDNA-CARy- aTM (aa291-404)
UseLentiviralTagsExpressionMammalianMutationn/aPromoterAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6C-N-term_F
Plasmid#146313PurposeMammalian Expression of HsTNRC6C-NtermDepositorInsertHsTNRC6C-Nterm (TNRC6C Human)
UseTagsExpressionMammalianMutationN548SPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK45 pHomL-RPL18Bp-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179029PurposeParent vector for markerless yeast integration cassettes with untagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK44 pHomL-TEF2p-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179028PurposeParent vector for markerless yeast integration cassettes with untagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRha-rsw-EYFP
Plasmid#182928PurposeEYFP driven by inducible promoter Prha-rswDepositorInsertEYFP
UseTagsExpressionMutationPromoterPrha-rswAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsAGO4_C
Plasmid#146085PurposeMammalian Expression of HsAGO4DepositorInsertHsAGO4 (AGO4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-RanGAP
Plasmid#175235PurposeBacterial expression and purification, high affinity SUMOylation substrateDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-Rhoa
Plasmid#163605PurposeExpresses RhoA Flp-dependently in mammalian cellsDepositorInsertRhoa (Rhoa Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-LifeAct-EGFP
Plasmid#163608PurposeExpresses LifeAct fused to EGFP Flp-dependently in mammalian cellsDepositorInsertLifeAct
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM62636
Plasmid#169095PurposeLow copy MoClo level P binary vector for plant transformationDepositorTypeEmpty backboneUseMoclo cloning vectorTagsExpressionPlantMutationPromoterAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
MvChR1_EYFP_pcDNA3.1
Plasmid#159119PurposeThis plasmid encodes MvChR1 rhodopsin domainDepositorInsertMvChR1
UseTagsEYFPExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP566
Plasmid#139500PurposeGene tagging vector to add mCherry Tag and membrane anchoring motifDepositorInsertmCherry-CaaX
UseTagsExpressionYeastMutationWTPromoterAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTEI125
Plasmid#126065PurposeModule 0 vector of PlaMiNGo toolkitDepositorInsertYFP1-10
UseSynthetic BiologyTagslinker (GTGGGGS)ExpressionMutationT205YPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHMGWA-Cas1
Plasmid#122285PurposeCas1 cloned in pHMGWADepositorInsertEco Cas1
UseTagsExpressionMutationPromoterAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS303 prp8-1
Plasmid#111306Purposeprp8-1DepositorInsertPRP8 (PRP8 Budding Yeast)
UseTagsExpressionYeastMutationprp8-1 ( G1801D )PromoterAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TetO-Snu114-fSNAPc
Plasmid#106989Purposeopen reading frame of Snu114 inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sitesDepositorInsertSnu114
UseTagsfSNAPExpressionMutationPromoterAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWT029g
Plasmid#96860Purposeguanine-agRNA (GFP activation, 18 nt blocker with bulges)DepositorInsertguanine-agRNA (GFP activation, 18 nt blocker with bulges)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWT029h
Plasmid#96861Purpose(d)guanine-agRNA (GFP activation,18 nt blocker with bulges)DepositorInsert(d)guanine-agRNA (GFP activation,18 nt blocker with bulges)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWT049d
Plasmid#96862Purposeguanine-agRNA (19 nt blocker with bulges, RFP activation)DepositorInsertguanine-agRNA (19 nt blocker with bulges, RFP activation)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWT049e
Plasmid#96863Purpose(d)guanine-agRNA (19 nt blocker with bulges, RFP activation)DepositorInsert(d)guanine-agRNA (19 nt blocker with bulges, RFP activation)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWT016h
Plasmid#96854PurposeHHR-bsgRNA-GFP (17 nt blocker)DepositorInsertHHR-bsgRNA-GFP (17 nt blocker)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWT016d
Plasmid#96855PurposedHHR-bsgRNA-GFP (17 nt blocker)DepositorInsertdHHR-bsgRNA-GFP (17 nt blocker)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWT016f
Plasmid#96850PurposeHHR-bsgRNA-GFP (10 nt blocker)DepositorInsertHHR-bsgRNA-GFP (10 nt blocker)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWT016e
Plasmid#96851PurposedHHR-bsgRNA-GFP (10 nt blocker)DepositorInsertdHHR-bsgRNA-GFP (10 nt blocker)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWT016a
Plasmid#96852PurposeHHR-bsgRNA-GFP (13 nt blocker)DepositorInsertHHR-bsgRNA-GFP (13 nt blocker)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWT016b
Plasmid#96853PurposedHHR-bsgRNA-GFP (13 nt blocker)DepositorInsertdHHR-bsgRNA-GFP (13 nt blocker)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB3
Plasmid#82027PurposeHybrid McpC[1-350]-Tar[267-553] expression plasmidDepositorInsertEncodes the hybrid receptor of E. coli chemoreceptor Tar and B. subtilis chemoreceptor McpC: McpC[1-350]-Tar[267-553]
UseTagsExpressionMutationPromoterAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB2
Plasmid#82026PurposeHybrid McpC[1-286]-Tar[200-553] expression plasmidDepositorInsertEncodes the hybrid receptor of E. coli chemoreceptor Tar and B. subtilis chemoreceptor McpC: McpC[1-286]-Tar[200-553]
UseTagsExpressionMutationPromoterAvailable sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB6
Plasmid#82028PurposeHybrid McpC[1-350]-YSYEH-Tar[272-553]DepositorInsertEncodes the hybrid receptor of E. coli chemoreceptor Tar and B. subtilis chemoreceptor McpC: McpC[1-350]-YSYEH-Tar[272-553]
UseTagsExpressionMutationPromoterAvailable sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB10
Plasmid#82039PurposeHybrid McpS[1-295]-Tar[200-553] expression plasmidDepositorInsertEncodes the hybrid receptor of E. coli chemoreceptor Tar and P. putida chemoreceptor McpS: McpS[1-295]-Tar[200-553]
UseTagsExpressionMutationPromoterAvailable sinceMarch 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB7
Plasmid#82029PurposeHybrid Tar[1-43]-CitA[55-173]-Tar[184-553] expression plasmidDepositorInsertEncodes the hybrid receptor of E. coli chemoreceptor Tar and histidine kinase CitA: Tar[1-43]-CitA[55-173]-Tar[184-553]
UseTagsExpressionMutationPromoterAvailable sinceMarch 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB9
Plasmid#82038PurposeHybrid ECA0434[1-385]-Tar[267-553] expression plasmidDepositorInsertEncodes the hybrid receptor of E. coli chemoreceptor Tar and P. atrosepticum chemoreceptor ECA0434: ECA0434[1-385]-Tar[267-553]
UseTagsExpressionMutationPromoterAvailable sinceMarch 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
miniUAA5
Plasmid#78484Purpose1336nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tailDepositorInsertCAN1-LUC
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
miniUAA6
Plasmid#78485Purpose1559nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tailDepositorInsertCAN1-LUC
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
miniUAA4
Plasmid#78483Purpose1105nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tailDepositorInsertCAN1-LUC
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
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miniUAA2
Plasmid#78481Purpose600nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tailDepositorInsertCAN1-LUC
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
miniUAA3
Plasmid#78482Purpose882nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tailDepositorInsertCAN1-LUC
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
wnt16-pCRII
Plasmid#68796PurposeFor in situ antisense probeDepositorInsertwnt16 (wnt16 Zebrafish)
UseTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMyo-3::oxCerulean-oxVenus::Lgg-1
Plasmid#68044PurposeLgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in body wall muscle, for monitoring autophagyDepositorInsertpMyo-3::oxCerulean-oxVenus::Lgg-1
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
PAS531 (TJF074)
Plasmid#65605PurposepETDuet-1 Containing FadD V4F, W5L -HisDepositorInsertFadD
UseTags6x HisExpressionBacterialMutationV4F, W5LPromoterT7 lacOAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only