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Plasmid#24431DepositorInsertKinesin WT (KIF5B Human)
UseTags6XHis and GFPExpressionBacterialMutationmonomeric kinesinPromoterAvailable sinceJune 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
RAB14 Q70L
Plasmid#61494Purposeto overexpress constitutively active RAB14. Vector includes GFP driven by hUbC promoterDepositorInsertRAB14 (RAB14 Human)
UseLentiviralTagsExpressionMutationQ70L, active formPromoterEF1aAvailable sinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.4) (RTW1028)
Plasmid#114084PurposeCAG promoter expression plasmid for human codon optimized enAsBE1.4DepositorInsertDNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.4)
UseTags2x SV40 NLS and SV40 NLSExpressionMammalianMutationE174R, S542R, K548R and D908A, human codon optimi…PromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTOPO-MCL1
Plasmid#21605DepositorInsertMCL-1 (MCL1 Human)
UseTagsExpressionMammalianMutationInsert ends with a STOP codon, no V5-His-tag.PromoterAvailable sinceAug. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pKmK.C1
Plasmid#125059PurposeStorage plasmid for K.marxianus centromereDepositorInsertKmARS7/CenD
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDL52 (clone45)
Plasmid#188578PurposeHigh-yield production of coenzyme F420 in E. coliDepositorInsertsribA
ribD
yigB
fbiC
cofC
cofD
cofI
cofE
UseTagsExpressionBacterialMutationCodon optimization, synthetic ribosome binding si…PromoterT7 variantsAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSiMPlc_N + pSiMPlc_C
Plasmid#134312PurposeMaintenance of 2 plasmids with a single antibiotic in E. coliDepositorInsertcmR_gp41-1 N-intein + cmR_gp41-1 C-intein
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_sfCherry2(11)_Keratin
Plasmid#82604PurposeExpresses sfCherry2(11) tagged keratin in mammalian cellsDepositorInsertsfCherry2(11)_Keratin
UseTagsExpressionMammalianMutationchanged Gly12 to Ala in sfCherry11+PromoterAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF1818
Plasmid#142860PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertUHRF1 (UHRF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-Cep63
Plasmid#136811PurposeMammalian expression of the centrosomal protein Cep63 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep63 (CEP63 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-EGFP-SHP2DA
Plasmid#12286DepositorInsertSHP-2DA (PTPN11 Human)
UseTagsMycExpressionMammalianMutationAspartic Acid 425 mutated to AlaninePromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
DT12A
Plasmid#80416PurposeExpresses 12 CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeatsDepositorInserthuman DMPK exons 11-15 with 12 CTG repeats (DMPK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmcherry-LCa
Plasmid#53972PurposeExpresses the light chain of clathrinDepositorInsertclathrin light chain
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pHH0103_GNAI1_G-alpha
Plasmid#110012PurposeProtein expression and purification of GNAI1_G-alphaDepositorInsertGNAI1_G-alpha (GNAI1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28b_Q69L
Plasmid#186272PurposeExpression vector of His-RanQ69L in E.coliDepositorInsertGTP-binding nuclear protein Ran (Ran Mouse)
UseTagsHis-tagged at N terminalExpressionBacterialMutationChanged Q69 into LPromoterT7 promoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbB9a-GFP
Plasmid#126112PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23113Available sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-mGFP
Plasmid#34831DepositorInsertLAMP1 (LAMP1 Human)
UseTagsGSTGSTGSTGA linker, Kozak, and mGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCZGY3163
Plasmid#101248PurposeGFP11::rps-18, ribosomal protein subunit, bound to C-terminal part of GFP. Shows GFP localized to ribosomes when combined with GFP 1-10.DepositorInsertgfp11::rps-18 (rps-18 Synthetic, Nematode)
UseGatewayTagsGFP11ExpressionMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
mApple-Rab5a-7
Plasmid#54944PurposeLocalization: Ras GTPase, Excitation: 568, Emission: 592DepositorInsertRab5a
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_HuD
Plasmid#65757Purposeexpresses FLAGHA-tagged HuD in mammalian cellsDepositorInsertELAVL4 (ELAVL4 Human)
UseTagsFLAG HA and V5ExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 U6-SaDMDR7-U6-SaDMDL2
Plasmid#78603PurposeAAV vector containing gRNAs (for SaCas9) targeting Dmd introns 22 and 23DepositorInsertU6-SaDMDR7-U6-SaDMDL2
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ6-LacI-Ptac(LacO)-LacZ
Plasmid#191621PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), IPTG-inducible promoter-lacZ, engineered from pMAL-c2XDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
H14_MBP_SUMO-Cys-linker-BAF
Plasmid#101777PurposeExpresses His-MBP-tagged BAF in E.coli (SUMO-cleavable)DepositorInsertBAF (BANF1) (BANF1 Human)
UseTagsHis14-MBP-SUMOExpressionBacterialMutationPromoterT5Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUA66 PcspA GFP No Linker
Plasmid#105605PurposeExpresses GFP under temperature sensitive promoter PcspADepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterPcspAAvailable sinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
IBMc090
Plasmid#161937PurposeCarries engineered transposon for IBMDepositorInsertpTHSSd_1V-WTR1R2-BbsI(G)-kanR-lacZalpha-SapI-WTR1R2
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS mouse IRS-1
Plasmid#11026DepositorInsertIRS-1 (Irs1 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pT2-Tbait-UAS-Positron-ST
Plasmid#129266PurposeUAS-driven expression of Positron in zebrafishDepositorInsertPositron-ST
UseZebrafish expressionTagsExpressionMutationD92N, E199VPromoterAvailable sinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCON1-ProD.mCherry
Plasmid#112522PurposeProD driven mCherry expression with synTTDepositorInsertProD mCherry TT
UseTagsExpressionBacterialMutationPromoterProDAvailable sinceJuly 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC-attB-burs-mCD8-EGFP-T2A-Gal4
Plasmid#39463DepositorInsertburs-mCD8-EGFP-T2A-Gal4
UseTagsExpressionMutationPromoterbursalphaAvailable sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
roGFP_iE_C147S_pQE30
Plasmid#48631PurposeBacterial expression vector encoding for the 6XHis tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 147 to Serine mutation (roGFP C147S, E147b, C204)DepositorInsertroGFP1
UseTagsHisTagExpressionBacterialMutationinsertion E147B, C147SPromoterAvailable sinceOct. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBbB12a-GFP
Plasmid#126115PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23151Available sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbB14a-GFP
Plasmid#126117PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbB11a-GFP
Plasmid#126114PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23150Available sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SFXN4
Plasmid#132280PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSFXN4 (SFXN4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-SUPREM
Plasmid#205023PurposeExpressing SUPer Rna Ecogii Methyltransferase (SUPREM)DepositorInsertSUPer Rna Ecogii Methyltransferase
UseTagsHis6x tagExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-mTagBFP2-2A-Puro_hU6-RfxDR36-BsmBI
Plasmid#226011PurposeCasRx guide RNA cloning backbone (lenti)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-mTagBFP2-2A-Puro_hU6-DJ-DR36-BsmBI
Plasmid#226009PurposeDjCas13d guide RNA cloning backbone (lenti)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB_EF1a-DjCas13d-msfGFP-Blast
Plasmid#226008PurposeDjCas13d protein for RNA targeting in piggyBac backboneDepositorInsertDjCas13d RNA-targeting nuclease
UseCRISPRTagsmsfGFPExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKM-T4-CMV-TnpB-optimized-P2A-GFP-Puro
Plasmid#205631PurposeExpression of TnpBmax in mammalian cellsDepositorInsertISDra2-TnpB optimized for mammalian expression
UseTagsNLS-3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only