-
Plasmid#219661PurposeGal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 5 destabilising mutations) fused to mGreenLanternDepositorInsertDestabilised ALFA nanobody (5 mutations) fused to mGreenLantern
UseTagsExpressionInsectMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB-dALFANb(3mut)-mGreenLantern
Plasmid#219662PurposeGal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 3 destabilising mutations) fused to mGreenLanternDepositorInsertDestabilised ALFA nanobody (3 mutations) fused to mGreenLantern
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-OCRL/INPP5b [N166A/26]
Plasmid#190301PurposeMammalian Expression Plasmid of anti-OCRL/INPP5b (Human). Derived from hybridoma N166A/26.DepositorInsertanti-OCRL/INPP5b (Homo sapiens) recombinant Mouse monoclonal antibody (OCRL Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-h53BP1 (siRNA resistant)
Plasmid#110301PurposeMammalian expression of a EGFP-tagged full length human 53BP1 (resistant to siRNA targeting AGAACGAGGAGACGGTAATAGTGGG)DepositorInsertp53-binding protein 1 (TP53BP1 Human)
UseTagsEGFPExpressionMammalianMutationGAACGAGGA to GAGCGGGGCPromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_3xFlagEzh2 RNA binding mutant
Plasmid#173718PurposeRNA-binding mutant EZH2 expression plasmid with 3XFLAG tagDepositorInsertEZH2 RNA-binding mutant
UseTags3XFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mCherry-CyrCR1.mutant
Plasmid#128746PurposeExpresses mCherry in mammalian cells. ~200 nt of Cyrano with 5 point mutations in miR-7 site inserted in mCherry 3'UTR.DepositorInsertCyrano conserved region (CR1) with mutant miR-7 site (OIP5-AS1 Human)
UseTagsExpressionMammalianMutation5 point mutations disrupting the miR-7 sitePromoterCMVAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-ArgiNLS-mGreenLantern
Plasmid#220596PurposeConstitutive expression of a single-cell discriminating version of mGreenLantern fluorescent protein.DepositorInsertmGreenLantern
UseAAVTagsArgiNLSExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-GluA2 [L21/32R]
Plasmid#177480PurposeMammalian Expression Plasmid of anti-GluA2/GluR2 glutamate receptor (Rat). Derived from hybridoma L21/32.DepositorInsertanti-GluA2/GluR2 glutamate receptor (Rattus norvegicus) recombinant mouse monoclonal antibody (Gria2 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Marathon-RT pentamutant for Split-PE (pEH107)
Plasmid#190110PurposeExpresses Marathon-RT pentamutant (D14R-N26R-D74R-N116K-N197R)DepositorInsertMarathon-RT
UseTagsbpNLSExpressionMammalianMutationD14R, N26R, D74R, N116K, N197RPromoterCMVAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
K-to-R mutant NPRL2(1-380) in pLJM60
Plasmid#184562PurposeCMV-driven expression of an untagged mutant NPRL2 (core subunit of GATOR1) — native sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.DepositorInsertNPRL2 (NPRL2 Human)
UseLentiviralTagsExpressionMammalianMutationAll lysines were mutated to arginines.PromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only