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Plasmid#68313PurposeMultisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafishDepositorTypeEmpty backboneUseZebrafish transgenesisTagsExpressionMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only
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p5'Entry-lck-7.4kb
Plasmid#58891Purposeprovides zebrafish lck promoter (7.4 kb) for GateWay construction of Tol2 transgenesDepositorInsertzebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 7.4 kb
UseGateway 5' entry cloneTagsExpressionMutationmutated single nucleotide (T-->C, 1874th nucle…Promoterlymphocyte-specific protein tyrosine kinase (lck)…Available sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TgKI-MCS-p2A-Venus-PEST-MCS-polyA
Plasmid#174030PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInsertp2A-Venus-PEST
UseZebrafish knock-in taggingTagsExpressionMutationPromoterAvailable sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
p5'Entry-lck-5.5kb
Plasmid#58890Purposeprovides zebrafish lck promoter (5.5 kb) for GateWay construction of Tol2 transgenesDepositorInsertzebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 5.5 kb
UseGateway 5' entry cloneTagsExpressionMutationmutated single nucleotide (T-->C, 13th nucleot…Promoterlymphocyte-specific protein tyrosine kinase (lck)…Available sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TgKI-MCS-p2A-mScarlet-MCS-polyA
Plasmid#174029PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInsertp2A-mScarlet
UseZebrafish knock-in taggingTagsExpressionMutationPromoterAvailable sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TgKI-MCS-mScarlet-MCS-polyA
Plasmid#174023PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInsertmScarlet
UseZebrafish knock-in taggingTagsExpressionMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
MLM3639
Plasmid#42252DepositorInsertStreptococcus pyogenes Cas9-3X Flag
UseCRISPR; Zebrafish expressionTags3X FlagExpressionMutationPromoterCMVAvailable sinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
Tol2_B-act_dCas9-Vp64-T2A-Citrine
Plasmid#200248PurposeZebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesisDepositorInsertdCas9-Vp64-T2A-Citrine
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TgKI-MCS-p2A-QF2-MCS-polyA
Plasmid#174026PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInsertp2A-QF2
UseZebrafish knock-in taggingTagsExpressionMutationPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHD157
Plasmid#84033PurposeVector for creating transgenic zebrafish. Expresses mCherry-Cre fusion protein under the fabp10 promoter and Venus under the crystalin promoter in zebrafishDepositorInsertsCre
Venus
UseCre/Lox; Zebrafish transgenesisTagsmCherryExpressionMutationPromoterCrystalin and fabp10Available sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-Cas9-pA-Cry-GFP
Plasmid#168239Purpose"neutrophil specific cas9 expression in zebrafish with green lens"DepositorInsertCas9
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKE13-ISceI-fabp10a:CreERT2
Plasmid#198242PurposeFor I-SceI-mediated transgenesis in zebrafish; fabp10a promoter driving tamoxifen-inducible Cre recombinaseDepositorInsertsUseZebrafish transgenesisTagsExpressionMutationPromoterAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Tol1.zf1
Plasmid#61388PurposemRNA synthesis of tol1 transposase codon modified for zebrafishDepositorInserttol1.zf1
UseZebrafish expressionTagsExpressionMutationPromotersp6Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Cre.zf1
Plasmid#61391PurposemRNA synthesis of Cre recombinase codon modified for zebrafishDepositorInsertCre.zf1
UseZebrafish expressionTagsExpressionMutationPromotersp6Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Gal4ff.zf1
Plasmid#61392PurposemRNA synthesis of Gal4ff codon modified for zebrafishDepositorInsertGal4ff.zf1
UseZebrafish expressionTagsExpressionMutationPromotersp6Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
punc503GFP
Plasmid#64021Purpose503-bp promoter of zebrafish unc-45b drives muscle-specific GFP expressionDepositorInsertunc-45b: GFP (unc45b Zebrafish)
UseGatewayTagsExpressionMutationPromoter503-bp promoter of zebrafish unc-45bAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pminiTol2-4kb Wnt3 promoter-EGFP
Plasmid#203792PurposeTol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of EGFP for zebrafish transgenesisDepositorInsertEGFP
UseZebrafish expressionTagsExpressionMutationPromoter4kb Wnt3 promoterAvailable sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-TagRFPT.zf1
Plasmid#61390PurposemRNA synthesis of TagRFPT codon modified for zebrafishDepositorInsertTagRFPT.zf1
UseZebrafish expressionTagsExpressionMutationPromotersp6Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2-NfsB.zf1
Plasmid#61387PurposemRNA synthesis of bacterial nitroreductase NfsB codon modified for zebrafishDepositorInsertnfsB.zf1
UseZebrafish expressionTagsExpressionMutationE28GPromotersp6Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Cer.zf1
Plasmid#61389PurposemRNA synthesis of cerulean codon modified for zebrafishDepositorInsertCer.zf1
UseZebrafish expressionTagsExpressionMutationPromotersp6Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only