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Plasmid#59738PurposeExpresses a.a. 417-887 of human ZO-1DepositorInsertzonula occludens-1 (TJP1 Human)
UseTagsHIS tag and thrombin clvgExpressionBacterialMutationPromoterT7Available sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G4_Q118R_Std_Dual_epegRNA_tevopreQ1
Plasmid#187454PurposeCoselection for prime editing in human cells. Vector for tandem expression of ATP1A1 Q118R-G4 pegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmidDepositorInsertATP1A1 G4 Q118R pegRNA (ATP1A1 Human)
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQTEV
Plasmid#31291DepositorInsert
UseTagsHis7 and TEVExpressionBacterialMutationPromoterAvailable sinceFeb. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G6_T804N_v1_Dual_epegRNA_tevopreQ1
Plasmid#187458PurposeCoselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 epegRNA (tevopreQ1) with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmidDepositorInsertATP1A1 G6 T804N epegRNA (tevopreQ1) (ATP1A1 Human)
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
P9_TEVs_cLuc
Plasmid#119303PurposeExpresses C-terminus of split luciferase connected to P9 coil via TEVs cleavable linker/for split protease-cleavable orthogonal Coiled-coil (SPOC) logicDepositorInsertsplit cLuc connected to P9 coil via TEVs cleavable linker
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_BauA
Plasmid#128952Purposeexpresses BauA in E.coliDepositorInsertferric acinetobactin receptor
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-66)PromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
nLuc_AP4_TEVs_P3mS
Plasmid#119299PurposeExpresses N-terminus of split luciferase fused to antiparallel coiled-coil AP4 connected with autoinhibitory coil P3mS and TEV cleavage site in antiparallel harpin linker/for SPOC logicDepositorInsertsplit nLuc fused to antiparallel Coiled-coil AP4 connected with P3 via TEV cleavable linker
UseLuciferaseTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmalc4x_I499F_TEV
Plasmid#47703PurposeExpresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation I499FDepositorInsertOlfactomedin-like domain with I499F mutation (MYOC Human)
UseTagsMBPExpressionBacterialMutationchanged I499FPromoterPtacAvailable sinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmalc4x_E323K_TEV
Plasmid#47699PurposeExpresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation E323KDepositorInsertMaltose Binding Protein and Olfactomedin-like domain with TEV cleavage site (MYOC Human)
UseTagsMBPExpressionBacterialMutationchanged glutamic acid 323 to lysinePromoterPtacAvailable sinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmalc4x_V426F_TEV
Plasmid#47702PurposeExpresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation V426FDepositorInsertOlfactomedin-like domain with V426F mutation (MYOC Human)
UseTagsMBPExpressionBacterialMutationchanged V426FPromoterPtacAvailable sinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G6_T804N_Std_Dual_epegRNA_tevopreQ1
Plasmid#187457PurposeCoselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 pegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmidDepositorInsertATP1A1 G6 T804N pegRNA (ATP1A1 Human)
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
HIS_TEV_hsSYX3
Plasmid#179290PurposeE. coli expression plasmid (T7lac promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + TEV protease cleavage siteDepositorInsertSyx
UseTagsHis6 + TEV protease cleavage siteExpressionBacterialMutationresidues 393-792 from accession number NP_0010361…PromoterT7lacAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeAR480A
Plasmid#128944Purposeexpresses R480A mutant of PfeA in E. coliDepositorInsertferric enterobactin receptor
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75), …PromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeAQ482A
Plasmid#128945Purposeexpresses Q482A mutant of PfeA in E. coliDepositorInsertferric enterobactin receptor
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75), …PromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeAG324V
Plasmid#128947Purposeexpresses G324V mutant of PfeA in E. coliDepositorInsertferric enterobactin receptor
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75), …PromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBADHISTEV_PfeEH258A
Plasmid#129122Purposeexpresses H258A mutant of PfeE in E.coliDepositorInsertPeriplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationSignal peptide sequence has been removed, mutat…PromoterPBADAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBADHISTEV_PfeES157A
Plasmid#129121Purposeexpresses S157A mutant of PfeE in E.coliDepositorInsertPeriplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationSignal peptide sequence has been removed, mutat…PromoterPBADAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmalc4x_G252R_TEV
Plasmid#47704PurposeExpresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation G252RDepositorInsertOlfactomedin-like domain with G252R mutation (MYOC Human)
UseTagsMBPExpressionBacterialMutationchanged Glycine 252 ArgininePromoterPtacAvailable sinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmalc4x_K398R_TEV
Plasmid#47701PurposeExpresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation K398RDepositorInsertOlfactomedin-like domain with K398R mutation (MYOC Human)
UseTagsMBPExpressionBacterialMutationchanged Lysine 398 to ArgininePromoterPtacAvailable sinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-RPL9
Plasmid#31334DepositorInsertribosomal protein L9 (RPL9 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only