Showing: 61 - 80 of 267 results
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Plasmid#15458DepositorInsert
UseS. aureus-e. coli shuttle vectorTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLI50
Plasmid#13573DepositorTypeEmpty backboneUseS. aureus-e. coli shuttle vectorTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCerulean
Plasmid#24756DepositorInsertCerulean
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
1782_pAAV-U6-Ai9-Sa-gRNA2-CB-SACas9-HA-OLLAS-spA_C
Plasmid#135978PurposegRNA against the right loxP site of the Ai9 alleleDepositorInsertAi9 gRNA2
UseAAV, CRISPR, and Mouse TargetingTagsHAExpressionMutationPromoterCBAvailabilityAcademic Institutions and Nonprofits only -
1781_pAAV-U6-Ai9-Sa-gRNA1-CB-SACas9-HA-OLLAS-spA_C
Plasmid#135979PurposegRNA against the left loxP site of the Ai9 alleleDepositorInsertAi9 gRNA1
UseAAV, CRISPR, and Mouse TargetingTagsHAExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA
Plasmid#61592PurposeA human codon-optimized Cas9 from Staphylococcus aureus (SaCas9).DepositorInserthSaCas9
UseAAV and CRISPRTags3xHA and NLSExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
MSP2262
Plasmid#70703PurposeBacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1DepositorInsertsmammalian codon-optimized Staphylococcus aureus dCas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationD10A & H557A in SaCas9PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
MSP2253
Plasmid#70704PurposeBacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1DepositorInsertsmammalian codon-optimized KKH variant Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationE782K, N968K and R1015H in SaCas9PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
MSP2279
Plasmid#70706PurposeBacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2DepositorInsertsmammalian codon-optimized Staphylococcus aureus dCas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationD10A and H557A in SaCas9PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
MSP2292
Plasmid#70707PurposeBacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2DepositorInsertsmammalian codon-optimized KKH variant Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationE782K, N968K and R1015H in SaCas9PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG-FLN-ELP-His-ybbr
Plasmid#168047PurposeExpresses SdrG with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminusDepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
UseTags3xELP linker, 6x Histag, ddFLN4, and ybbr tagExpressionBacterialMutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 gfaABC1D-NLS-SaCas9-3xHA-NLS bGH
Plasmid#178960PurposeExpresses saCas9 protein specifically in astrocytesDepositorInsertStaphylococcus aureus Cas9 (SaCas9)
UseAAVTags3x HAExpressionMutationPromotergfaABC1D (astrocyte specific)AvailabilityAcademic Institutions and Nonprofits only -
pRMC2-msfGFP
Plasmid#194913PurposeTetracycline inducible expression of msfGFP in StaphylococciDepositorInsertShine Dalgarno Sequence followed by monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…TagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLL2787
Plasmid#15460DepositorInsertphage phi11 int gene
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pmRFPmars
Plasmid#26252DepositorInsertmRFPmars
UseTags6xHisExpressionBacterialMutationKpnI restriction site removedPromoterAvailabilityAcademic Institutions and Nonprofits only -
JF001A
Plasmid#53116Purposeexpresses alpha hemolysin under the control of theophylline riboswitchDepositorInserttheophylline riboswitch and alpha hemolysin
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pCAG-CFP-SaCas9-HF (without sgRNA)
Plasmid#134470PurposeHuman expression vector for SaCas9-HF variant.DepositorInsertStaphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
UseCRISPRTagsSV40NLS-3xFLAGExpressionMammalianMutationR245A, N413A, N419A, R654APromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pX601-mCherry
Plasmid#84039PurposeStaphylococcus aureus (SaCas9) conjugated with mCherryDepositorInsertSaCas9
UseAAV and CRISPRTagsNLS and T2A-mCherryExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pX601-GFP
Plasmid#84040PurposeStaphylococcus aureus (SaCas9) conjugated with GFPDepositorInsertSaCas9
UseAAV and CRISPRTagsNLS and T2A-EGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pRMC2-sec:msfGFP
Plasmid#194914PurposeTetracycline inducible expression of msfGFP fused to Sec signal peptide in StaphylococciDepositorInsertShine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…TagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 61 - 80 of 267 results