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EcMBP165-cpGFP.PPYF.pRSET

Plasmid

#33372

Purpose

a single-wavelength maltose sensor constructed from E. coli MalE and cpGFP

Depositor

Loren Looger

Article

Marvin et al Proteins. 2011 Nov;79(11):3025-36. doi:

Insert

MBP based Maltose Biosensor

Use

Tags

6xHIS

Expression

Bacterial

Mutation

cpGFP146 variant from GCaMP2 inserted between AA1…

Promoter

T7

Available since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pRSET-B CFP

Plasmid

#108858

Purpose

Encodes for the fluorescent protein CFP

Depositor

Kalina Hristova

Article

Sarabipour et al Anal Biochem. 2014 Mar 15;449:155-7. doi

Insert

CFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET-B YFP

Plasmid

#108856

Purpose

Encodes for the fluorescent protein enhanced YFP (eYFP)

Depositor

Kalina Hristova

Article

Sarabipour et al Anal Biochem. 2014 Mar 15;449:155-7. doi

Insert

eYFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pRSETb-rsGreen0.7

Plasmid

#78176

Purpose

Expresses rsGreen0.7 in E. coli strains.

Depositor

Peter Dedecker

Article

Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

Insert

rsGreen0.7

Use

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 27, 2016

Availability

Academic Institutions and Nonprofits only

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pRSETb-ffDronpa

Plasmid

#78182

Purpose

Expresses ffDronpa E. coli strains

Depositor

Peter Dedecker

Article

Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

Insert

ffDronpa

Use

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 27, 2016

Availability

Academic Institutions and Nonprofits only

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Lapronic/pRSETB

Plasmid

#140755

Purpose

FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio

Depositor

Luis Felipe Barros

Article

Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

Insert

Lapronic (Lactate/PYRuvate Ratio Optical Nano-Indicator from CECs)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pRSETB-de4GFP

Plasmid

#85472

Purpose

Inducible expression of dual emitting pH sensitive GFP in bacterial cells

Depositor

S. James Remington

Article

Hanson et al Biochemistry. 2002 Dec 31;41(52):15477-8

Insert

de4GFP

Use

Tags

6xHis

Expression

Bacterial

Mutation

S65T/C48S/H148C/T203C

Promoter

T7

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pRSETB-ro2GFP

Plasmid

#82366

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

Insert

ro2GFP

Use

Tags

6xHIS

Expression

Bacterial

Mutation

C48S/S65T/Q80R/S147C/Q204C

Promoter

T7

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pRSETb-pcDronpa2

Plasmid

#78184

Purpose

Expresses pcDronpa2 in E. coli strains

Depositor

Peter Dedecker

Article

Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

Insert

pcDronpa2

Use

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pRSET-B-frSkyran-S

Plasmid

#182209

Purpose

Bacterial expression of frSkylan-S

Depositor

Shiro Suetsugu

Article

Osuga et al Mol Biol Cell. 2021 Nov 1;32(21):br7. do

Insert

frSkylan-S

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPmal-2u

Plasmid

#14479

Depositor

Wolf Frommer

Article

Fehr et al Proc Natl Acad Sci U S A. 2002 Jul 23. 9

Insert

FLIPmal

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

5AA

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pRSET-XS-VWF

Plasmid

#64847

Purpose

Expresses aa1594–1670 of VWF A2 domain flanked by Venus and Cerulean and tagged with 7X His at C terminus

Depositor

Sriram Neelamegham

Article

Dayananda et al Anal Biochem. 2011 Mar 15;410(2):206-13.

Insert

Venus_VWF A2 domain aa 1594-1670_Cerulean_TEV_7X His (VWF Human, Synthetic)

Use

Tags

7X His, Cerulean, and Venus

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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pRSETb-rsGreen1

Plasmid

#78180

Purpose

Expresses rsGreen1 in E. coli strains.

Depositor

Peter Dedecker

Article

Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

Insert

rsGreen1

Use

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 27, 2016

Availability

Academic Institutions and Nonprofits only

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Kohinoor/pRSETb

Plasmid

#67770

Purpose

Kohinoor for bacterial expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
TN-XXL pRSETB

Plasmid

#45796

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Mank et al Nat Methods. 2008 Sep;5(9):805-11. doi:

Insert

TN-XXL

Use

Tags

6xHIS, ECFP, and YFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only
pRSETB-ro1GFP

Plasmid

#82365

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

Insert

ro1GFP

Use

Tags

6xHIS

Expression

Bacterial

Mutation

C48S/Q80R/S147C/Q204C

Promoter

T7

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPPi-4u

Plasmid

#13553

Depositor

Wolf Frommer

Article

Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

Insert

FLIPPi T163A

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

T163A in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pRSETB-roGFP1-R7

Plasmid

#83309

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Cannon et al Protein Sci. 2006 Jan;15(1):45-57. Epub

Insert

roGFP1-R7

Use

Tags

6xHIS

Expression

Bacterial

Mutation

C48S/Q80R/S147C/Q204C/F223R/S202K

Promoter

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only
Twitch-3 pRSETB

Plasmid

#48204

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-3A

Use

Tags

6xHIS, cpVenusCD, and mCerulean3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only
pRSETB-ro4GFP

Plasmid

#82368

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

Insert

ro4GFP

Use

Tags

6xHIS

Expression

Bacterial

Mutation

C48S/S65T/Q80R/N149C/S202C

Promoter

T7

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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