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Showing: 61 - 80 of 589 results

  1. pET28a(+)::NL3F10H

    Plasmid
    #141290
    Purpose
    NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)
    Depositor
    Andrew Millar
    Article
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Insert
    NLUC3F10H
    Use
    Luciferase
    Tags
    Flag (x3) and His (x10)
    Expression
    Bacterial
    Mutation
    Engineered for high stability (t1/2 = 11.5 days a…
    Promoter
    promoter for bacteriophage T7 RNA polymerase
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    141290-plasmid-map-sequence-id-324909

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  2. pET28a(+)::MBP-NL3F10H

    Plasmid
    #141291
    Purpose
    MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)
    Depositor
    Andrew Millar
    Article
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Insert
    MBP-NLUC3F10H
    Use
    Luciferase
    Tags
    Flag (x3), His (x10), and MBP
    Expression
    Bacterial
    Mutation
    Engineered for high stability (t1/2 = 11.5 days a…
    Promoter
    promoter for bacteriophage T7 RNA polymerase
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    141291-plasmid-map-sequence-id-324616

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  3. pET28a Gx-NL3

    Plasmid
    #169108
    Purpose
    Expresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.
    Depositor
    Maarten Merkx
    Article
    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66
    Insert
    protein G - NanoLuc - NanoLuc - NanoLuc
    Use
    Tags
    his-tag and strep-tag
    Expression
    Bacterial
    Mutation
    Ala 24 stop
    Promoter
    T7
    Available since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    169108-plasmid-map-sequence-id-335659

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  4. pCDH-EF1-N-cG-Blast

    Plasmid
    #124981
    Purpose
    Express Nanoluc, copGFP
    Depositor
    Kazuhiro Oka
    Insert
    Nanoluc, copGFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1-alpha
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    124981-plasmid-map-sequence-id-243977

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  5. pCDH-EF1-N-cG-Hph

    Plasmid
    #124980
    Purpose
    Express Nanoluc, copGFP
    Depositor
    Kazuhiro Oka
    Insert
    Nanoluc, copGFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1-alpha
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    124980-plasmid-map-sequence-id-243976

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  6. pCDH-EF1-N-cG-Zeo

    Plasmid
    #124979
    Purpose
    Express Nanoluc, copGFP
    Depositor
    Kazuhiro Oka
    Insert
    Nanoluc, copGFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1-alpha
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    124979-plasmid-map-sequence-id-243974

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  7. pCDH-TRE3G-N-tdT-CMV-P

    Plasmid
    #192887
    Purpose
    Tet-inducible Nanoluc-tdTomato expression
    Depositor
    Kazuhiro Oka
    Insert
    Nanoluc-P2A-tdTomato
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    192887-plasmid-map-sequence-id-393170

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  8. pCDH-EF1-N-cG-Neo

    Plasmid
    #124978
    Purpose
    Express Nanoluc, copGFP
    Depositor
    Kazuhiro Oka
    Insert
    Nanoluc, copGFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1-alpha
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    124978-plasmid-map-sequence-id-243972

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  9. pCDH-TRE3G-N-cG-CMV-P

    Plasmid
    #192886
    Purpose
    Tet inducible Nanoluc copGFP expression
    Depositor
    Kazuhiro Oka
    Insert
    Nanoluc-P2A-copGFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    192886-plasmid-map-sequence-id-378891

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  10. pCDH-CMV5-N-cG-P

    Plasmid
    #192883
    Purpose
    coexpression of Nanoluc, copGFP and puromycin resistant marker
    Depositor
    Kazuhiro Oka
    Insert
    Nanoluc-P2A-copGFP-T2A-Puro
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    192883-plasmid-map-sequence-id-378927

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  17. pET28a Gx-NL2

    Plasmid
    #169107
    Purpose
    Expresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.
    Depositor
    Maarten Merkx
    Article
    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66
    Insert
    protein G - NanoLuc - NanoLuc
    Use
    Tags
    his-tag and strep-tag
    Expression
    Bacterial
    Mutation
    Ala 24 stop
    Promoter
    T7
    Available since
    June 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    169107-plasmid-map-sequence-id-334716

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  20. pBH502

    Plasmid
    #215781
    Purpose
    NBU2 backbone with LacI(YQR) and P(O1)_sgRNA targeting Nanoluc
    Depositor
    Corey Wilson
    Article
    Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:
    Inserts
    LacI(YQR)
    sgRNA-ribozyme cassette
    Nanoluc
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Bt1311, P(O1), and cfxA
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    215781-plasmid-map-sequence-id-430541

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Showing: 61 - 80 of 589 results