Purpose
All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).
Inserts
Csy4-T2A-SpRFN-P2A-GFP
gRNA
Use
CRISPR
Tags
Csy4, Csy4 recognition sequence, FokI fusion via …
Expression
Mammalian
Mutation
D10A, H840A
Available since
Jan. 20, 2017
Availability
Academic Institutions and Nonprofits only